A membrane-based fluorescence-quenching immunochromatographic sensor for the rapid detection of tetrodotoxin

Shen, Haicong; Zhang, Shiwei; Fu, Qiangqiang; Xiao, Wei; Wang, Shifeng; Yu, Shiting; Xiao, Meng; Bian, Hongfen; Tang, Yong

doi:10.1016/j.foodcont.2017.06.001

Cited by 24 publications

(10 citation statements)

References 23 publications

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“…Studies have shown that different tissues in different animals have different resistance to TTX (Figure 2) [97,100]. To date, at least nine different sodium channel isoforms have been identified in the mammalian nervous system [101,102]. Three of these, Na v 1.5 (IC 50 = 5.7 µM), Na v 1.8 (IC 50 = 60 µM), and Na v 1.9 (IC 50 = 40 µM), are resistant to TTX (IC 50 = 5.7 µM).…”

Section: Mode Of Action Of Ttxmentioning

confidence: 99%

“…TTX is a potent neurotoxin well known for its ability to inhibit the voltage-sensitive sodium channel with potential primary blockade of the brainstem, somatic motor, sensory, and autonomic nerves [103]. Recent studies have determined that voltage-gated sodium channels not only play an important role in the normal electrophysiological activities of neurons but also have a close relationship with neurological diseases [101]. After the discovery of the blocking property of Na channels in nerve cells of the TTX molecule in the 1960s, the studies mostly shifted to the cellular and molecular mechanisms of TTX [98,104].…”

Section: Mode Of Action Of Ttxmentioning

confidence: 99%

“…To avoid subjectivity and provide more precise measurements, LFAs can be coupled to portable readers. This is the case of the works where fluorescence quenching was used as a detection method [101,314].…”

Section: Immunoassays Immunosensors and Immunostripsmentioning

confidence: 99%

See 2 more Smart Citations

An Updated Review of Tetrodotoxin and Its Peculiarities

et al. 2022

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Tetrodotoxin (TTX) is a crystalline, weakly basic, colorless organic substance and is one of the most potent marine toxins known. Although TTX was first isolated from pufferfish, it has been found in numerous other marine organisms and a few terrestrial species. Moreover, tetrodotoxication is still an important health problem today, as TTX has no known antidote. TTX poisonings were most commonly reported from Japan, Thailand, and China, but today the risk of TTX poisoning is spreading around the world. Recent studies have shown that TTX-containing fish are being found in other regions of the Pacific and in the Indian Ocean, as well as the Mediterranean Sea. This review aims to summarize pertinent information available to date on the structure, origin, distribution, mechanism of action of TTX and analytical methods used for the detection of TTX, as well as on TTX-containing organisms, symptoms of TTX poisoning, and incidence worldwide.

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Section: Mode Of Action Of Ttxmentioning

confidence: 99%

Section: Mode Of Action Of Ttxmentioning

confidence: 99%

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An Updated Review of Tetrodotoxin and Its Peculiarities

et al. 2022

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“…Fluorescence strategies have been highly studied in the field of biotoxin monitoring. Many examples include the use of indirect detection by competitive assays [ 85 , 86 , 88 , 89 , 93 ], quenching of the fluorescent signal [ 87 , 90 , 127 ], sandwich assays [ 84 ], and aptamer binding [ 92 ]. Fluorescent biosensors have been developed not only for single detection but also for multiplex detection.…”

Section: Optical Biosensors For Detecting Aquatic Biotoxinsmentioning

confidence: 99%

Emerging Optical Materials in Sensing and Discovery of Bioactive Compounds

Vaz

Valpradinhos

Frasco

et al. 2021

Sensors

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Optical biosensors are used in numerous applications and analytical fields. Advances in these sensor platforms offer high sensitivity, selectivity, miniaturization, and real-time analysis, among many other advantages. Research into bioactive natural products serves both to protect against potentially dangerous toxic compounds and to promote pharmacological innovation in drug discovery, as these compounds have unique chemical compositions that may be characterized by greater safety and efficacy. However, conventional methods for detecting these biomolecules have drawbacks, as they are time-consuming and expensive. As an alternative, optical biosensors offer a faster, simpler, and less expensive means of detecting various biomolecules of clinical interest. In this review, an overview of recent developments in optical biosensors for the detection and monitoring of aquatic biotoxins to prevent public health risks is first provided. In addition, the advantages and applicability of these biosensors in the field of drug discovery, including high-throughput screening, are discussed. The contribution of the investigated technological advances in the timely and sensitive detection of biotoxins while deciphering the pathways to discover bioactive compounds with great health-promoting prospects is envisaged to meet the increasing demands of healthcare systems.

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“…Nevertheless, the further application of LFA is impeded by poor sensitivity (Chen et al, 2015). To improve the sensitivity, researchers have replaced the traditional label-AuNP by AuNP nanocomposite and novel nanoparticle, including AuNP-decorated silica nanorods (Xu et al, 2014), quantum dots (Qu et al, 2016), fluorescence-quenching (Shen et al, 2017), and so on, which involved sophisticated preparation procedures and advanced detection tools. Meanwhile, the advances based on silver staining (Rodríguez et al, 2016), enzyme labeling (Cho and Irudayaraj, 2013), or NH 2 OH•HCl enlargement (Li et al, 2013) have been widely used to amplify the detection signals; however, these methods rely on additional procedures to add extra reagents and extend the detection time (Chen et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Gold nanoparticle-based enhanced lateral flow immunoassay for detection of Cronobacter sakazakii in powdered infant formula

Pan¹,

Jiang²,

Sun

et al. 2018

Journal of Dairy Science

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Cronobacter sakazakii is an opportunistic foodborne pathogen that can infect newborns through powdered infant formula (PIF). In this study, we developed a novel enhanced lateral flow immunoassay (LFA) with enhanced sensitivity for detection of C. sakazakii in PIF by the naked eye. The proposed strategy for signal enhancement of the traditional LFA used concentrated gold nanoparticles (AuNP) as the enhancer to conjugate with capture antibodies, which could increase the immobilized capture antibodies concentration at the detection zone to improve capture efficiency. Besides, the detection signal was further amplified by accumulated AuNP as the C. sakazakii labeled with AuNP probes was captured by antibodies conjugated with enhancer at the test line. We also studied the effect of different concentrations of capture antibodies and concentrated AuNP on detection performance, and found that 2.2 mg/mL of capture antibodies and 0.06 nM concentrated AuNP were the optimal combination that could avoid a false-positive signal and maximally amplify the detection signal of the enhanced LFA. Using this strategy, the detection sensitivity of the enhanced LFA was 10 cfu/mL and improved 100-fold compared with traditional LFA. The strip was highly specific to C. sakazakii, and the time for detection of C. sakazakii in PIF was shortened by 3 h. In summary, the enhanced LFA developed by the addition of concentrated AuNP as the enhancer can be used as a sensitive, rapid, visual qualitative and point-of-care test method for detecting target analytes.

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A membrane-based fluorescence-quenching immunochromatographic sensor for the rapid detection of tetrodotoxin

Cited by 24 publications

References 23 publications

An Updated Review of Tetrodotoxin and Its Peculiarities

An Updated Review of Tetrodotoxin and Its Peculiarities

Emerging Optical Materials in Sensing and Discovery of Bioactive Compounds

Gold nanoparticle-based enhanced lateral flow immunoassay for detection of Cronobacter sakazakii in powdered infant formula

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