A membraneless starch/O2 biofuel cell based on bacterial surface regulable displayed sequential enzymes of glucoamylase and glucose dehydrogenase

Cai, Yuanyuan; Wang, Mingyang; Xiao, Xinxin; Liang, Bo; Fan, Shumin; Zheng, Zongmei; Cosnier, Serge; Liu, Aihua

doi:10.1016/j.bios.2022.114197

Cited by 9 publications

(7 citation statements)

References 37 publications

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“…The use of enzyme cascades is an alternative to maintaining the high energy densities of biofuel cells and increasing energy density. Enzyme cascades can mimic the metabolic pathways of enzymes to completely oxidize substrates such as ethanol and increase power density by almost ten times compared to a single enzyme ethanol biofuel cell [ 294 , 295 , 296 , 297 , 298 , 299 , 300 , 301 , 302 , 303 , 304 ].…”

Section: Discussion and Resultsmentioning

confidence: 99%

Biofuels and Nanocatalysts: Python Boosting Visualization of Similarities

et al. 2023

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Among the most relevant themes of modernity, using renewable resources to produce biofuels attracts several countries’ attention, constituting a vital part of the global geopolitical chessboard since humanity’s energy needs will grow faster and faster. Fortunately, advances in personal computing associated with free and open-source software production facilitate this work of prospecting and understanding complex scenarios. Thus, for the development of this work, the keywords “biofuel” and “nanocatalyst” were delivered to the Scopus database, which returned 1071 scientific articles. The titles and abstracts of these papers were saved in Research Information Systems (RIS) format and submitted to automatic analysis via the Visualization of Similarities Method implemented in VOSviewer 1.6.18 software. Then, the data extracted from the VOSviewer were processed by software written in Python, which allowed the use of the network data generated by the Visualization of Similarities Method. Thus, it was possible to establish the relationships for the pair between the nodes of all clusters classified by Link Strength Between Items or Terms (LSBI) or by year. Indeed, other associations should arouse particular interest in the readers. However, here, the option was for a numerical criterion. However, all data are freely available, and stakeholders can infer other specific connections directly. Therefore, this innovative approach allowed inferring that the most recent pairs of terms associate the need to produce biofuels from microorganisms’ oils besides cerium oxide nanoparticles to improve the performance of fuel mixtures by reducing the emission of hydrocarbons (HC) and oxides of nitrogen (NOx).

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Section: Discussion and Resultsmentioning

confidence: 99%

Biofuels and Nanocatalysts: Python Boosting Visualization of Similarities

et al. 2023

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“…This is usually achieved by adding a localization tag to the scaffold protein (Wang and Yu 2012 ; Chen et al 2022 ). Furthermore, protein scaffolds allow for control and optimization of spatial organization, stoichiometry, and enzyme proximity, which is particularly important when flux balance correction is required (Whitaker and Dueber 2011 ; Cai et al 2022 ). The main problem of protein scaffolds is the time-consuming protein labeling and the associated genetic engineering.…”

Section: Strategies Of Enzyme Immobilization Mediated By a Scaffoldmentioning

confidence: 99%

“…E Generating bioelectricity from cellodextrin with CDP–PGM enzyme complex (Meng et al 2019 ). F Preparation a starch-oxidizing bioanode based on displaying a sequential enzyme system of GA–GDH on E. coli cell surfaces in a precise way using cohesin–dockerin interactions (Cai et al 2022 ). G Synthetic scaffolds built from modular protein–protein interaction domains (Dueber et al 2009 ).…”

Section: Strategies Of Enzyme Immobilization Mediated By a Scaffoldmentioning

confidence: 99%

Research progress of multi-enzyme complexes based on the design of scaffold protein

Wang,

Jiang,

Liu

et al. 2023

Bioresour. Bioprocess.

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Multi-enzyme complexes designed based on scaffold proteins are a current topic in molecular enzyme engineering. They have been gradually applied to increase the production of enzyme cascades, thereby achieving effective biosynthetic pathways. This paper reviews the recent progress in the design strategy and application of multi-enzyme complexes. First, the metabolic channels in the multi-enzyme complex have been introduced, and the construction strategies of the multi-enzyme complex emerging in recent years have been summarized. Then, the discovered enzyme cascades related to scaffold proteins are discussed, emphasizing on the influence of the linker on the fusion enzyme (fusion protein) and its possible mechanism. This review is expected to provide a more theoretical basis for the modification of multi-enzyme complexes and broaden their applications in synthetic biology.

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“…Nuclear magnetic resonance. 1 H NMR spectra was used to detect the conformation of the hydrolyzates 7 .…”

Section: Metal Content Analysismentioning

confidence: 99%

“…Glucoamylase (GA) (EC 3.2.1.3, α-l, 4-glucan glucohydrolase), one of the most extensively utilized enzymes in industry, cleaves α-1,4-glycosidic linkages and α-1,6-glycosidic linkages from the non-reducing end of oligosaccharide substrates and starch to release β- d -glucose 1 , 2 . Glycoside hydrolases (GHs) are a class of enzymes that can hydrolyze glycosidic bonds in carbohydrates, and glucoamylases are mainly distributed in GH15 and GH97 family according to the carbohydrate-active enzyme (CAZy) database ( http://www.cazy.org ) 3 .…”

Section: Introductionmentioning

confidence: 99%

The effect of barium and strontium on activity of glucoamylase QsGH97a from Qipengyuania seohaensis SW-135

Zhai

Chen

et al. 2023

Sci Rep

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Glycoside hydrolases (GHs), the enzymes that break glycosidic bonds, are ubiquitous in the ecosystem, where they perform a range of biological functions. As an interesting glycosidase family, Glycoside hydrolase family 97 (GH97) contains α-glucosidase, α-galactosidase, and glucoamylase. Only ten members of GH97 have been characterized so far. It is critical to explore novel members to elucidate the catalytic mechanism and application potential of GH97 family. In this study, a novel glucoamylase QsGH97a from Qipengyuania seohaensis SW-135 was cloned and expressed in E. coli. Sequence analysis and NMR results show that QsGH97a is classified into GH97a, and adopts inverting mechanism. The biochemical characterization indicates that QsGH97a shows the optimal activity at 50 °C and pH 8.0. Ca2+ has little effect on the catalytic activity; however, the activity can be substantially increased by 8–13 folds in the presence of Ba2+ or Sr2+. Additionally, the metal content of QsGH97a assay showed a high proportion of Sr2+. The specific metal activity was initially revealed in glucoamylases, which is not found in other members. These results imply that QsGH97a not only is a new member of GH97, but also has potential for industrial applications. Our study reveals that Ba2+ or Sr2+ may be involved in the catalytic mechanism of glucoamylase, laying the groundwork for a more complete knowledge of GH97 and its possible industrial application.

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A membraneless starch/O2 biofuel cell based on bacterial surface regulable displayed sequential enzymes of glucoamylase and glucose dehydrogenase

Cited by 9 publications

References 37 publications

Biofuels and Nanocatalysts: Python Boosting Visualization of Similarities

Biofuels and Nanocatalysts: Python Boosting Visualization of Similarities

Research progress of multi-enzyme complexes based on the design of scaffold protein

The effect of barium and strontium on activity of glucoamylase QsGH97a from Qipengyuania seohaensis SW-135

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