A mesofluidic multiplex immunosensor for detection of circulating cytokeratin-positive cells in the blood of breast cancer patients

Breton, François; Bennetau, Bernard; Lidereau, Rosette; Thomas, L.; Regnier, Jérémi; Ehrhart, Jean-Claude; Tauc, Patrick; Tran, Phuong-Lan

doi:10.1007/s10544-010-9465-7

Cited by 10 publications

(8 citation statements)

References 30 publications

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“…The WIVT is an assembly that includes a pressure transducer, signal conditioner, and wireless components encapsulated in an elastomeric capsule filled with silicone gel [6]. The base station is a custom designed portable electronics transceiver, worn by the participant, which wirelessly receives IAP data from the WIVT and stores the data on a microSD card.…”

Section: Methodsmentioning

confidence: 99%

“…For example, self-regulated, ambulatory activities cannot be monitored in a urogynecology laboratory setting. Additionally, the standard fluid-filled rectal balloon catheter used in such urodynamics studies is not conducive to real-world settings due to their poor dynamic response and encumbered mobility [6, 7]. …”

Section: Introductionmentioning

confidence: 99%

“…To overcome the limitations of currently available technology, our group developed a wireless intra-vaginal transducer (WIVT) which approximates IAP by measuring pressure in the upper vagina [6]. Data from the WIVT was found to correlate well with the simultaneous measurement of a rectal balloon catheter for coughing and Valsalva maneuvers in a clinical laboratory setting [8].…”

Section: Introductionmentioning

confidence: 99%

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Effects of walking speeds and carrying techniques on intra-abdominal pressure in women

et al. 2014

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Aims To test the feasibility of monitoring intra-abdominal pressure (IAP) outside the laboratory environment and to compare IAP while 1) carrying 13.6 kg (similar to a 3-month old in car seat) in 6 different ways while walking 100 meters; and 2) walking 400 meters at self-selected slow, normal, and fast pace. Methods Forty-six healthy women between 19 and 54 years completed the walking and lifting activities; the order for each was randomized. IAP was monitored with an intra-vaginal pressure transducer that wirelessly transmitted pressure data to a portable base station. We analyzed maximal peak IAP and area under the curve (AUC) IAP. Results Monitoring IAP outside of the laboratory was feasible. Mean maximal IAP during walking increased as pace increased; 42.5 (SD 10.2), 50.5 (10.9), and 62.0 (12.1) cm H2O for slow, medium, and fast speeds, respectively (p < 0.0001 by mixed model ANOVA). The corresponding AUC of IAP for walking decreased as pace increased. The ‘awkward carry’, ‘side carry’, and ‘front carry’ activities each resulted in higher mean maximal IAP (65.8 (10.6), 67.7 (12.8), 77.3 (13.1) cm H2O, respectively) than the ‘carry in backpack’ activity (55.5 (11.4) cm H2O; p < 0.0001). Conclusion Subtle variations in walking speed or method of carrying a toddler-size load can produce significant changes in IAP. Whether these increase the risk of pelvic floor disorders is not yet clear. However, these data suggest further inquiry into optimal methods and appliances to assist women in carrying may enable achieving a lower IAP profile.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of walking speeds and carrying techniques on intra-abdominal pressure in women

et al. 2014

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“…IAP was monitored by the wireless remote abdominal pressure system which consists of the sensor and a portable base station. The pressure sensor contains a piezoresistive pressure sensor, microcontroller, and wireless transceiver that is sealed in an elastomeric capsule measuring 23.9 mm in diameter and 37.3 mm in length filled with silicone gel [9]. Data, captured at 31Hz, from the pressure sensor was sent wirelessly to a portable base station located on the participant’s hip that stored data on a microSD card.…”

Section: Methodsmentioning

confidence: 99%

“…Most of these post-surgical activity restrictions are based upon individual viewpoints and vary widely in strenuousness and duration [8]. To study IAP during physical activity, we developed a wireless remote intravaginal pressure system [9, 10]. …”

Section: Introductionmentioning

confidence: 99%

Intra-abdominal pressure during Pilates: unlikely to cause pelvic floor harm

et al. 2015

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Aims To describe intra-abdominal pressures (IAP) generated during Pilates Mat and Reformer activities, and determine whether these activities generate IAP above a sit-to-stand threshold. Methods Twenty healthy women with no symptomatic vaginal bulge, median age 43 (range 22 – 59 years), completed Pilates Mat and Reformer exercise routines each consisting of 11 exercises. IAP was collected by an intra-vaginal pressure transducer, transmitted wirelessly to a base station, and analyzed for maximal and area under the curve (AUC) IAP. Results There were no statistically significant differences in mean max IAP between sit-to-stand and any of the Mat or Reformer exercises in the study population. Six to twenty-five percent of participants exceeded their individual mean max IAP sit-to-stand thresholds for 10 of the 22 exercises. When measuring AUC from 0 cm H2O, half the exercises exceeded the mean AUC of sit-to-stand but only Pilates Reformer and Mat roll-ups exceeded the mean AUC of sit-to-stand when calculated from a threshold of 40 cm H2O (consistent with, for example, walking). Conclusion Our results support recommending this series of introductory Pilates exercises including five Mat exercises and six Reformer exercises to women desiring a low IAP exercise routine. More research is needed to determine the long term effects of Pilates exercise on post-surgical exercise rehabilitation and pelvic floor health.

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Folic Acid Functionalized Surface Highlights 5‐Methylcytosine‐Genomic Content within Circulating Tumor Cells

Malara

Coluccio

Limongi

et al. 2014

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Although the detection of methylated cell free DNA represents one of the most promising approaches for relapse risk assessment in cancer patients, the low concentration of cell-free circulating DNA constitutes the biggest obstacle in the development of DNA methylation-based biomarkers from blood. This paper describes a method for the measurement of genomic methylation content directly on circulating tumor cells (CTC), which could be used to deceive the aforementioned problem. Since CTC are disease related blood-based biomarkers, they result essential to monitor tumor's stadiation, therapy, and early relapsing lesions. Within surface's bio-functionalization and cell's isolation procedure standardization, the presented approach reveals a singular ability to detect high 5-methylcytosine CTC-subset content in the whole CTC compound, by choosing folic acid (FA) as transducer molecule. Sensitivity and specificity, calculated for FA functionalized surface (FA-surface), result respectively on about 83% and 60%. FA-surface, allowing the detection and characterization of early metastatic dissemination, provides a unique advance in the comprehension of tumors progression and dissemination confirming the presence of CTC and its association with high risk of relapse. This functionalized surface identifying and quantifying high 5-methylcytosine CTC-subset content into the patient's blood lead significant progress in cancer risk assessment, also providing a novel therapeutic strategy.

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A mesofluidic multiplex immunosensor for detection of circulating cytokeratin-positive cells in the blood of breast cancer patients

Cited by 10 publications

References 30 publications

Effects of walking speeds and carrying techniques on intra-abdominal pressure in women

Effects of walking speeds and carrying techniques on intra-abdominal pressure in women

Intra-abdominal pressure during Pilates: unlikely to cause pelvic floor harm

Folic Acid Functionalized Surface Highlights 5‐Methylcytosine‐Genomic Content within Circulating Tumor Cells

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