A Mesoporous Silica Nanosphere-Based Carrier System with Chemically Removable CdS Nanoparticle Caps for Stimuli-Responsive Controlled Release of Neurotransmitters and Drug Molecules

Lai, Chian Sing; Trewyn, Brian G.; Jeftinija, Dušan M.; Jeftinija, Ksenija; Xu, Shiping; Jeftinija, Srdija; Lin, Victor S.‐Y.

doi:10.1021/ja028650l

Cited by 1,650 publications

(1,203 citation statements)

References 32 publications

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“…s-MSN was prepared by our previously reported method. 33 In brief, N-cetyltrimethylammonium bromide (CTAB, 1.00 g, 2.74 mmol) was dissolved in 480 mL of nanopure water, followed by the addition of 3. , and 3-(triethoxysilyl)propylsuccinic anhydride of various amount (x mmol) in anhydrous toluene (50 mL) for 20 h, followed by filtration and washing with toluene and methanol, and dried under high vacuum overnight. The succinic anhydride groups were hydrolyzed by boiling the materials in water for 6 h and measured by FTIR.…”

Section: Methodsmentioning

confidence: 99%

Interaction of Mesoporous Silica Nanoparticles with Human Red Blood Cell Membranes: Size and Surface Effects

Zhao¹,

Sun²,

Zhang³

et al. 2011

ACS Nano

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The interactions of mesoporous silica nanoparticles (MSNs) of different particle sizes and surface properties with human red blood cell (RBC) membranes were investigated by membrane filtration, flow cytometry, and various microscopic techniques. Small MCM-41-type MSNs (∼100 nm) were found to adsorb to the surface of RBCs without disturbing the membrane or morphology. In contrast, adsorption of large SBA-15-type MSNs (∼600 nm) to RBCs induced a strong local membrane deformation leading to spiculation of RBCs, internalization of the particles, and eventual hemolysis. In addition, the relationship between the degree of MSN surface functionalization and the degree of its interaction with RBC, as well as the effect of RBC−MSN interaction on cellular deformability, were investigated. The results presented here provide a better understanding of the mechanisms of RBC−MSN interaction and the hemolytic activity of MSNs and will assist in the rational design of hemocompatible MSNs for intravenous drug delivery and in vivo imaging. R ecent advancements in particle size and morphology control of mesoporous materials have led to the creation of nano-and submicrometer-sized mesoporous silica nanoparticles (MSNs). [1][2][3][4][5] The MSN materials with well-ordered cylindrical pore structures, such as MCM-41 and SBA-15, have attracted special interest in the biomedical field. 1 The large surface areas and pore volumes of these materials allow the efficient adsorption of a wide range of molecules, including small drugs, 6-10 therapeutic proteins, 11-13 antibiotics, 14,15 and antibodies. 16 Therefore, these materials have been proposed for use as potential vehicles for biomedical imaging, real-time diagnosis, and controlled delivery of multiple therapeutic agents. [6][7][8]10,[17][18][19][20][21][22][23][24][25] Despite the considerable interest in the biomedical applications of MSNs, relatively few studies have been published on the biocompatibility of the two most common types of MSNs (MCM-41 and SBA-15). [26][27][28][29] Asefa and co-workers reported that the cellular bioenergetics (cellular respiration and ATP levels) were inhibited remarkably by large SBA-15 nanoparticles, but the inhibition was greatly reduced by smaller MCM-41-type nanoparticles. 26 These differences in the disruption of cellular bioenergetics are believed to be caused by the different surface areas, number of surface silanol groups, and/or particle sizes of both types of material. A recent study by Kohane and collaborators on the systemic effects of MCM-41 (particle size ∼150 nm) and SBA-15 (particle size ∼800 nm) MSNs in live animals revealed interesting findings regarding their biocompatibility. 27 While large doses of mesoporous silicas administered subcutaneously to mice appear to be relatively harmless, the same doses given intravenously or intraperitoneally were lethal. 27 A possible reason for the severe systemic toxicity of MSNs when injected intravenously could be the interactions of the nanoparticles with blood cells.Our initial studies...

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Section: Methodsmentioning

confidence: 99%

Interaction of Mesoporous Silica Nanoparticles with Human Red Blood Cell Membranes: Size and Surface Effects

Zhao¹,

Sun²,

Zhang³

et al. 2011

ACS Nano

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516

440

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“…23,24 As described in the Supporting Information (SI), the material was PEGylated by grafting 2-[methoxy(polyethylenoxy)propyl]trimethoxysilane to yield the PEGylated (0.1 mmol g À1 ) linker-MSN with an average particle diameter of 160 nm and an MCM-41-type channel-like mesoporous structure (BJH pore diameter = 2.5 nm) ( Figure 2a).…”

mentioning

confidence: 99%

“…In addition, the disulfide linkage design renders the release of luciferin predominately inside the cells. 9,23,24 The most advantageous feature of this Au-MSN platform is the potential to deliver different biogenic species simultaneously in a controlled fashion. To construct the enzymeÀsubstrate codelivery system, luciferase was immobilized on the external surface of Au-MSNs by incubating luciferin-loaded Au-MSNs (1 mg mL À1 ) with luciferase (1 mg mL À1 ) in PBS buffer (pH 7.4) for 2 h, followed by centrifugation and freeze-drying.…”

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Luciferase and Luciferin Co-immobilized Mesoporous Silica Nanoparticle Materials for Intracellular Biocatalysis

et al. 2011

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We report a gold nanoparticle (AuNP)-capped mesoporous silica nanoparticle (Au-MSN) platform for intracellular codelivery of an enzyme and a substrate with retention of bioactivity. As a proof-of-concept demonstration, Au-MSNs are shown to release luciferin from the interior pores of MSN upon AuNP uncapping in response to disulfide-reducing antioxidants and codeliver bioactive luciferase from the PEGylated exterior surface of Au-MSN to Hela cells. The effectiveness of luciferase-catalyzed luciferin oxidation and luminescence emission in the presence of intracellular ATP was measured by a luminometer. Overall, the chemical tailorability of the Au-MSN platform to retain enzyme bioactivity, the ability to codeliver enzyme and substrate, and the potential for imaging tumor growth and metastasis afforded by intracellular ATP-and glutathione-dependent bioluminescence make this platform appealing for intracellular controlled catalysis and tumor imaging. ABSTRACT: We report a gold nanoparticle (AuNP)-capped mesoporous silica nanoparticle (Au-MSN) platform for intracellular codelivery of an enzyme and a substrate with retention of bioactivity. As a proof-of-concept demonstration, Au-MSNs are shown to release luciferin from the interior pores of MSN upon AuNP uncapping in response to disulfide-reducing antioxidants and codeliver bioactive luciferase from the PEGylated exterior surface of Au-MSN to Hela cells. The effectiveness of luciferase-catalyzed luciferin oxidation and luminescence emission in the presence of intracellular ATP was measured by a luminometer. Overall, the chemical tailorability of the Au-MSN platform to retain enzyme bioactivity, the ability to codeliver enzyme and substrate, and the potential for imaging tumor growth and metastasis afforded by intracellular ATP-and glutathionedependent bioluminescence make this platform appealing for intracellular controlled catalysis and tumor imaging.

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“…Recently, several MSN-based controlled-release systems with the "zero-premature release" property have been synthesized by using different kinds of pore-blocking caps, such as nanoparticles (NPs), [5][6][7] organic molecules, 8,9 and supramolecular assemblies. 10,11 Different stimuli-responsive strategies, such as chemical, 5 pH, 9,12,13 electrostaticinteraction, 14 enzymatic, 15 redox, 16 andphotoirradiation, [17][18][19][20][21] have been applied as "triggers" for uncapping the pores and releasing the guest molecules from MSNs.…”

mentioning

confidence: 99%

“…10,11 Different stimuli-responsive strategies, such as chemical, 5 pH, 9,12,13 electrostaticinteraction, 14 enzymatic, 15 redox, 16 andphotoirradiation, [17][18][19][20][21] have been applied as "triggers" for uncapping the pores and releasing the guest molecules from MSNs. Despite these burgeoning developments, many of the MSN-based controlled release systems possess only one of the two features, but not both, and are unable to function under physiological conditions.…”

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confidence: 99%

Photoinduced Intracellular Controlled Release Drug Delivery in Human Cells by Gold-Capped Mesoporous Silica Nanosphere

et al. 2009

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A gold nanoparticle (AuNP)-capped mesoporous silica nanosphere (MSN)-based intracellular drug delivery system (PR-AuNPs-MSN) for the photoinduced controlled release of an anticancer drug, paclitaxel, inside of human fibroblast and liver cells was synthesized and characterized. We found that the mesopores of MSN could be efficiently capped by the photoresponsive AuNPs without leaking the toxic drug, paclitaxel, inside of live human cells. This “zero premature release” characteristic is of importance for delivery of toxic drugs in chemotherapy. Furthermore, we demonstrated that the cargo-release property of this PR-AuNPs-MSN system could be easily controlled by low-power photoirradiation under biocompatible and physiological conditions. We envision that our results would play a significant role in designing new generations of carrier materials for intracellular delivery of a variety of hydrophobic toxic drugs.

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A Mesoporous Silica Nanosphere-Based Carrier System with Chemically Removable CdS Nanoparticle Caps for Stimuli-Responsive Controlled Release of Neurotransmitters and Drug Molecules

Cited by 1,650 publications

References 32 publications

Interaction of Mesoporous Silica Nanoparticles with Human Red Blood Cell Membranes: Size and Surface Effects

Interaction of Mesoporous Silica Nanoparticles with Human Red Blood Cell Membranes: Size and Surface Effects

Luciferase and Luciferin Co-immobilized Mesoporous Silica Nanoparticle Materials for Intracellular Biocatalysis

Photoinduced Intracellular Controlled Release Drug Delivery in Human Cells by Gold-Capped Mesoporous Silica Nanosphere

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