A meta-analysis of microRNA expression profiling studies in heart failure

Gholaminejad, Alieh; Zare, Nasrin; Dana, Nasim; Shafie, Davood; Mani, Arya; Javanmard, Shaghayegh Haghjooy

doi:10.1007/s10741-020-10071-9

Cited by 35 publications

(25 citation statements)

References 130 publications

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“…We employed the VCR strategy to identify the consensus list of altered miRNAs in LN condition. The VCR is a simple meta-analysis method applied for combining differentially expressed pro les coming from individual studies (20)(21)(22). In addition, for the validation of the combined results, using meta-analysis of p values, the statistical signi cance of the differential expression of each meta-miRNA was calculated.…”

Section: Discussionmentioning

confidence: 99%

Candidate microRNA Biomarkers in Lupus Nephritis: A Meta-Analysis of Profiling Studies in Kidney, Blood and Urine Samples

Roointan

Gholaminejad

Shojaie

et al. 2022

Preprint

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Lupus nephritis (LN) is a kidney disease caused by systemic lupus erythematosus in which kidneys are attacked by the immune system. MiRNAs participate in the pathogenesis of LN as modulatory effectors in immune responses and nephrogenesis pathways. Despite the large number of miRNAs that are expressed deferentially, identifying the most suitable biomarker candidate for LN is challenging. The aim of this study was to introduce a consensus panel of potential miRNA biomarkers by performing a meta-analysis of miRNA profiles in the kidney, blood, and urine samples of LN patients. A comprehensive literature review approach was considered to find LN-related miRNA expression profiles. After including the 41 eligible studies and performing the data extraction, meta-analysis was done based on the vote-counting rank strategy and as well as meta-analysis of p-value. The result of the meta-analysis was three lists of consensus miRNAs with altered expression profiles in the various tissue samples of LN patients. Of the 13 studies on kidney tissue, the meta-miRNAs were let-7a, miR-198, let-7e, miR-145, and miR-26a. In addition, meta-miRNAs of miR-199a, miR-21, miR-423, miR-1260b, miR-589, miR-150, miR-155, miR-146a, and miR-183 from 21 studies on blood samples, and miR-146a, miR-204, miR-30c, miR-3201, and miR-1273e from 11 studies on urine samples can be considered as non-invasive biomarker panels for LN. The meta-miRNAs and their related genes were subjected to functional enrichment analyses and network construction. Functional enrichment analysis confirmed the involvement of their target genes in nephropathy-related signaling pathways. Network construction showed miR-145-5p in blood and miR-155-5p in kidney, the best hob miRNAs as candidate drug targets. In conclusion using a meta-analysis approach, our study proposes three meta-miRNA panels that could be the target of further research to assess their potential as biomarkers / therapeutic targets in LN disease.

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Section: Discussionmentioning

confidence: 99%

Candidate microRNA Biomarkers in Lupus Nephritis: A Meta-Analysis of Profiling Studies in Kidney, Blood and Urine Samples

Roointan

Gholaminejad

Shojaie

et al. 2022

Preprint

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“…The targeting property of nanoformulations highlights a unique advantage in the treatment of cardio-cerebrovascular diseases, and carrier PEG-PLGA plays an important role in the development of innovative nanomedicines because it is safe and nontoxic. Gholaminejad A ( Gholaminejad et al, 2021 ) found that dysregulated expression of mir-30b-5p was closely related to heart failure occurrence by bioinformatics analysis. Wang et al (2015) discovered a novel signalling pathway composed of E2F1, miR-30b, and CypD, which could regulate myocardial necrosis and subsequently provide a new direction for the effective treatment of myocardial infarction and heart failure.…”

Section: Discussionmentioning

confidence: 99%

Mechanism of miR-30b-5p-Loaded PEG-PLGA Nanoparticles for Targeted Treatment of Heart Failure

et al. 2021

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Objective: Exploring the effectiveness of miR-30b-5p-loaded PEG-PLGA nanoparticles (NPs) for the treatment of heart failure and the underlying mechanism.Methods: PEG-PLGA characteristics with different loading amounts were first examined to determine the loading, encapsulation, and release of miR-30b-5p from NPs. The effects of miR-30b-5p NPs on cardiac function and structure were assessed by immunofluorescence, echocardiography, HE/Masson staining, and TUNEL staining. The effects of NPs on the expression of factors related to cardiac hypertrophy and inflammation were examined by RT-PCR and western blotting, and the mechanism of miR-30b-5p treatment on heart failure was explored by dual luciferase reporter assay and RT-PCR.Results: The size of PEG-PLGA NPs with different loading amounts ranged from 200 to 300 nm, and the zeta potential of PEG-PLGA NPs was negative. The mean entrapment efficiency of the NPs for miR-30b-5p was high (81.8 ± 2.1%), and the release rate reached 5 days with more than 90% release. Distribution experiments showed that NPs were mainly distributed in the heart and had a protective effect on myocardial injury and cardiac function. Compared with a rat model of cardiac failure and miR-30b-5p-non-loaede NP groups, the expression of cardiac hypertrophy markers (ANP, BNPβ-MHC) and inflammatory factors (IL-1β, IL-6) were significantly decreased. Dual luciferase reporter assay assays indicated that miR-30b-5p exerted its effects mainly by targeting TGFBR2.Conclusion: PEG-PLGA NPs loaded with miR-30b-5p improved cardiac function, attenuated myocardial injury, and regulated the expression of factors associated with cardiac hypertrophy and inflammation by targeting TGFBR2.

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“…miRs are a large class of non-coding RNA that play a critical role at the post transcriptional level, by binding to complementary mRNAs at specific sequences located within the 3′UTR of target mRNA, leading to transcriptional inhibition or degradation [ 71 ] miRs are found to be dysregulated in a range of disorders including cardiovascular disease, diabetes and cancer [ 72 , 73 , 74 , 75 , 76 , 77 ]. There is a growing body of evidence that an abundance of miRs play an important role in regulation of Nox (see recent review by Wlodarski et al [ 78 ]).…”

Section: Regulation Of Noxmentioning

confidence: 99%

Nicotinamide Adenine Dinucleotide Phosphate Oxidases in Glucose Homeostasis and Diabetes-Related Endothelial Cell Dysfunction

Brown

Bridge

Kearney

2021

Cells

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Oxidative stress within the vascular endothelium, due to excess generation of reactive oxygen species (ROS), is thought to be fundamental to the initiation and progression of the cardiovascular complications of type 2 diabetes mellitus. The term ROS encompasses a variety of chemical species including superoxide anion (O2•-), hydroxyl radical (OH-) and hydrogen peroxide (H2O2). While constitutive generation of low concentrations of ROS are indispensable for normal cellular function, excess O2•- can result in irreversible tissue damage. Excess ROS generation is catalysed by xanthine oxidase, uncoupled nitric oxide synthases, the mitochondrial electron transport chain and the nicotinamide adenosine dinucleotide phosphate (NADPH) oxidases. Amongst enzymatic sources of O2•- the Nox2 isoform of NADPH oxidase is thought to be critical to the oxidative stress found in type 2 diabetes mellitus. In contrast, the transcriptionally regulated Nox4 isoform, which generates H2O2, may fulfil a protective role and contribute to normal glucose homeostasis. This review describes the key roles of Nox2 and Nox4, as well as Nox1 and Nox5, in glucose homeostasis, endothelial function and oxidative stress, with a key focus on how they are regulated in health, and dysregulated in type 2 diabetes mellitus.

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A meta-analysis of microRNA expression profiling studies in heart failure

Cited by 35 publications

References 130 publications

Candidate microRNA Biomarkers in Lupus Nephritis: A Meta-Analysis of Profiling Studies in Kidney, Blood and Urine Samples

Candidate microRNA Biomarkers in Lupus Nephritis: A Meta-Analysis of Profiling Studies in Kidney, Blood and Urine Samples

Mechanism of miR-30b-5p-Loaded PEG-PLGA Nanoparticles for Targeted Treatment of Heart Failure

Nicotinamide Adenine Dinucleotide Phosphate Oxidases in Glucose Homeostasis and Diabetes-Related Endothelial Cell Dysfunction

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