A Meta-Analysis of the 16S-rRNA Gut Microbiome Data in Honeybees (<i>Apis Mellifera</i>)

Gkantiragas, Alexis; Gabrielli, Jacopo

doi:10.1101/2021.12.18.473299

Cited by 4 publications

(3 citation statements)

References 66 publications

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“…Rhizobiaceae (Rhizobium), the most well-known nitrogen-fixing bacterium, is recognized as an unusual bacterium found in the honeybee gut. Researchers believe that these are likely Gilliamella , which belongs to the order Rhizobium [ 63 ]. However, others have retained this genus as Rhizobiaceae [ 64 ].…”

Section: Discussionmentioning

confidence: 99%

Gut Microbiota of Apis mellifera at Selected Ontogenetic Stages and Their Immunogenic Potential during Summer

Maigoro,

Lee,

Kim

et al. 2024

Pathogens

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Honeybees (Apis mellifera) are pollinating agents of economic importance. The role of the gut microbiome in honeybee health has become increasingly evident due to its relationship with immune function, growth, and development. Although their dynamics at various developmental stages have been documented, their dynamics during the era of colony collapse disorder and immunogenic potential, which are connected to the antagonistic immune response against pathogens, need to be elucidated. Using 16S rRNA gene Illumina sequencing, the results indicated changes in the gut microbiota with the developmental stage. The bacterial diversity of fifth stage larva was significantly different among the other age groups, in which Fructobacillus, Escherichia-Shigella, Bombella, and Tyzzerella were unique bacteria. In addition, the diversity of the worker bee microbiome was distinct from that of the younger microbiome. Lactobacillus and Gilliamella remained conserved throughout the developmental stages, while Bifidobacterium colonized only worker bees. Using an in silico approach, the production potential of lipopolysaccharide-endotoxin was predicted. Forager bees tend to have a higher abundance rate of Gram-negative bacteria. Our results revealed the evolutionary importance of some microbiome from the larval stage to the adult stage, providing insight into the potential dynamics of disease response and susceptibility. This finding provides a theoretical foundation for furthering the understanding of the function of the gut microbiota at various developmental stages related to probiotic development and immunogenic potential.

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Section: Discussionmentioning

confidence: 99%

Gut Microbiota of Apis mellifera at Selected Ontogenetic Stages and Their Immunogenic Potential during Summer

Maigoro,

Lee,

Kim

et al. 2024

Pathogens

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“…Honey bees belong to the insect order Hymenoptera, in the suborder Apocrita and the Apidae family, and are eusocial insects that are critically important for the maintenance of diversity in global ecosystems [28][29][30][31]. It is estimated that pollinators contribute at least EUR 22 billion per year to European agriculture [32,33].…”

Section: Bee Species and Their Products 21 Bee Speciesmentioning

confidence: 99%

Microbial Diversity in Bee Species and Bee Products: Pseudomonads Contribution to Bee Well-Being and the Biological Activity Exerted by Honey Bee Products: A Narrative Review

Tsadila,

Amoroso,

Mossialos

2023

Diversity

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Bees are important pollinators worldwide, promoting sustainability in agriculture and natural ecosystems. Moreover, honey bees produce a variety of honey bee products (beehive products). Honey is the main edible bee product. The consumption of pollen, bee bread, royal jelly, and propolis is becoming more popular nowadays. All these products are characterized by high nutritional value and/or bioactivity. A high microbial diversity has been reported in bees and beehive products, forming distinct microbial communities. The honey bee gut microbiome actively promotes good health and nutrient availability for the host. Furthermore, it prevents food spoilage and contributes to the maintenance of good hygiene conditions in the hive. Pseudomonads are often reported in investigations on bee and bee product microbiomes. Diverse Pseudomonas species demonstrate high metabolic adaptability, producing a wide range of bioactive enzymes and secondary metabolites. Several studies have provided evidence that Pseudomonads might play a role in bee well-being and the bioactivity exerted by honey bee products, though further research is warranted to fully understand the effects and mechanisms. The aim of this narrative review is to highlight the importance of Pseudomonads in the context of up-to-date knowledge regarding the bee and bee product microbiomes.

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“…It is important to highlight that, researchers do come across some unusual bacteria in the honey bee microbiome including the mentioned Rhizobium, which is well-known as nitrogen-fixing bacteria found in root nodules of legume plants [4]. These are likely Gillamella belonging to the order Rhizobium, yet are considered as part of the honey bee core microbiome [31].…”

Section: Gut Microbiota Taxonomy Diversity Based On Different Diets I...mentioning

confidence: 99%

Different Diets Modulate the Gut Microbiome Compositions and Promote the Health ofApis mellifera

Kim,

Maigoro,

Lee

et al. 2023

Preprint

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Honey bee (Apis mellifera) health is crucial for honey bee products, and effective pollination and is closely associated with gut bacteria. Various factors such as reduced habitat, temperature, disease, and diet affect the health of honey bees, by disturbing the homeostasis of the gut microbiota. In this study, high-throughput 16S rRNA gene sequencing was used to analyze the gut microbiota ofApis melliferasubjected to seven different diets. The identified microbiota in theApis melliferagut from all the diets consisted ofLactobacillus(62%), followed byRhizobiaceae(21%),Snodgrassella(4%), andErwiniaceae(4%) among other 33 genera. Based on diet types,Lactobacillusa lactic acid bacteria (LAB), dominates the microbiota with the highest relative abundance in AIGT+SAC (91%), AIGT+Soytide (88%), and AIGT+Apple juice (69%) diet groups.BifidobacteriumandCommensalibacterappeared as the second most abundant genera in AIGT+SAC and AIGT+Soytide diet groups, respectively. These bacteria are important markers for honey bee health. Considering the importance of these diets in shaping their host microbiome into a healthy status. Individual honey bee health (IHH) was observed to validate the quality and correlation between the microbiota and honey bee health. The results were consistent, indicating thatApis melliferafed on AIGT+Soytide and AIGT+SAC diet showed the highest health expression level of vitellogenin. The group with 60%Syrup possessingRhizobiaceaeas the dominant taxa showed poor health status. This finding paved the way for establishing a link between gut microbiota and IHH under different diets.

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A Meta-Analysis of the 16S-rRNA Gut Microbiome Data in Honeybees (Apis Mellifera)

Cited by 4 publications

References 66 publications

Gut Microbiota of Apis mellifera at Selected Ontogenetic Stages and Their Immunogenic Potential during Summer

Gut Microbiota of Apis mellifera at Selected Ontogenetic Stages and Their Immunogenic Potential during Summer

Microbial Diversity in Bee Species and Bee Products: Pseudomonads Contribution to Bee Well-Being and the Biological Activity Exerted by Honey Bee Products: A Narrative Review

Different Diets Modulate the Gut Microbiome Compositions and Promote the Health ofApis mellifera

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