A Metabolomic Analysis of Very Long Chain Fatty Acid Synthesis in Control and Refsum Fibroblasts

Abstract

insulin, increases skeletal muscle concentration of mTOR, p70S6k, 4E-BP1, and eIF4E. Increases in phosphorylated, activated mTOR and 4E-BP1 were only demonstrated when insulin concentrations were also increased. These results suggest that increased supply of AA maximizes muscle protein synthesis not just by mass effect of increased substrate, but by increasing the total amount of critical regulatory proteins, thereby augmenting the effect of insulin to activate these proteins by phosphorylation.