A metabolomic, transcriptomic profiling, and mineral nutrient metabolism study of the phytotoxicity mechanism of uranium

Lai, Jin-long; Liu, Ze-wei; Luo, Xin

doi:10.1016/j.jhazmat.2019.121437

Cited by 68 publications

(34 citation statements)

References 57 publications

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“…The accumulation of U in the apoplast, which is a temperature-insensitive adsorption and diffusion process (Barberon and Geldner, 2014), accounts for 60-70% bioaccumulated U. This is in agreement with previous experiments showing that plant roots or plant cells exposed to U accumulate the radionuclide principally in the cell wall and intracellular spaces (Laurette et al, 2012a;Laurette et al, 2012b;El Hayek et al, 2018;El Hayek et al, 2019;Lai et al, 2020;Sarthou et al, 2020;Wu et al, 2020). The absorption of U by the symplastic pathway, which is mainly temperature-sensitive (reduction of energy metabolism, enzymatic activities, membrane fluidity, plasmodesmatal permeability) (Barberon and Geldner, 2014;Sager and Lee, 2014), accounts for 30-40% bioaccumulated U, and is virtually fully inhibited by external Ca at high concentration (Figure 3) or by inhibitors of NSCCs and Ca 2+ -permeable cation channels (Figure 4).…”

Section: Discussionsupporting

confidence: 91%

“…Uranium has been shown to interfere with several aspects of plant physiology, development and metabolism (Misson et al, 2009;Saenen et al, 2013;Aranjuelo et al, 2014;Vanhoudt et al, 2014;Serre et al, 2019). Biochemical, molecular and metabolomic studies indicated that U triggers oxidative stress-related response pathways and perturbs hormones synthesis and signaling, primary metabolism, iron and phosphate assimilation pathways, and cell wall synthesis (Vanhoudt et al, 2011c;Vanhoudt et al, 2011a;Doustaly et al, 2014;Saenen et al, 2015;Tewari et al, 2015;Lai et al, 2020;Lai et al, 2021). Also, U has a significant impact on mineral nutrition, with important changes in some micro-and macronutrients concentration and distribution between roots and shoots (Misson et al, 2009;Vanhoudt et al, 2011b;Doustaly et al, 2014;Berthet et al, 2018;Lai et al, 2020;Sarthou et al, 2020;Lai et al, 2021;Rajabi et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Calcium-permeable cation channels are involved in uranium uptake in Arabidopsis thaliana

Sarthou

Devime

Baggio

et al. 2021

Preprint

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Uranium (U) is a non-essential and toxic element that is taken up by plants from the environment. The assimilation pathway of U is still unknown in plants and any other organism. In this study, we provide several evidences that U is taken up by the roots of Arabidopsis thaliana through Ca2+-permeable cation channels. First, we showed that deprivation of Arabidopsis plants with calcium induced a 1.5-fold increase in the capacity of roots to accumulate U, suggesting that calcium deficiency promoted the radionuclide import pathway. Second, we showed that external calcium inhibits U accumulation in roots, suggesting a common route for the uptake of both cations. Third, we found that gadolinium, nifedipine and verapamil inhibit the absorption of U, suggesting that different types of Ca2+-permeable channels serve as a route for U uptake. Last, we showed that U bioaccumulation in Arabidopsis mutants deficient for the Ca2+-permeable channels MCA1 and ANN1 was decreased by 40%. This suggests that MCA1 and ANN1 contribute to the absorption of U in different zones and cell layers of the root. Together, our results describe for the first time the involvement of Ca2+-permeable cation channels in the cellular uptake of U.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Calcium-permeable cation channels are involved in uranium uptake in Arabidopsis thaliana

Sarthou

Devime

Baggio

et al. 2021

Preprint

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“…19,20 Also, U perturbs iron, phosphate and manganese homeostasis 9,14,21,22 and triggers important changes in the metabolome. 23 At the molecular level, U has been shown to alter the expression of genes involved in iron and phosphate homeostasis, hormone synthesis and signalling, and cell wall metabolism in Arabidopsis thaliana. 21 Vicia faba plants challenged with U also displayed important changes of their transcriptome.…”

Section: Introductionmentioning

confidence: 99%

“…21 Vicia faba plants challenged with U also displayed important changes of their transcriptome. 23 Despite these studies, the consequences of uranyl on the dynamics of proteomes and the molecular targets of U are still not known in plants. Since uranyl is able to bind strongly to biomolecules via carboxylate, phosphate or sulphate moieties, proteins are expected to be the primary targets of U.…”

Section: Introductionmentioning

confidence: 99%

Development of a metalloproteomic approach to analyse the response of Arabidopsis cells to uranium stress

et al. 2020

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“…The samples were analyzed on an Agilent 7890B gas chromatography system coupled to a 5977A MSD system (Agilent, Guangzhou, China) according to Lai et al [32]. The metabolites were annotated using the Fiehn or NIST database [33].…”

Section: Untargeted Metabolomics Analysis Of Storage Rootsmentioning

confidence: 99%

Metabonomic Profiling Analyses Reveal ANS Upregulation to Enhance the Flavonoid Pathway of Purple-Fleshed Sweet Potato Storage Root in Response to Deep Shading

Zhu

Sun

et al. 2021

Agronomy

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Intercropping, as a common worldwide cultivation pattern, provides opportunities for sustainable agriculture with fuller use of light, temperature and land resources and greater yield per unit of land. The intercropping impact on crop quality is a current focus. This study found that shading cultivation of purple-fleshed sweet potato can improve the storage root pigment accumulation by more than 20% to increase economic benefits. We performed gas chromatography and mass spectrometry analysis of storage roots of the anthocyanin-enriched cultivar Jihei-1 under 60% shading and nonshaded treatments. A total of 224 differential metabolites were identified, among which N-acetyl-5-hydroxytryptamine, 1-monopalmitin, 4-pyridoxic acid, dodecano, arbutin, tryptophan, citrulline and phenylalanine were significantly upregulated under shading with a more than 10-fold change. Furthermore, metabolic pathway enrichment maps were based on the biological processes and stratification level selected. These metabolites mainly influenced the pathways of phenylpropanoid biosynthesis, the citrate cycle, organic acid biosynthesis and metabolism and amino acid metabolism. Through tissue-specific dynamic changes in amino acids, soluble sugars, starch and anthocyanins during storage root development, we proposed a variety-specific strategy of purple-fleshed sweet potato in response to prolonged deep shading, that is, utilizing and enhancing broad aboveground-tissue photosynthesis and transferring photosynthates into roots in advance, leading to a rapid increase in storage root anthocyanin synthesis. With comprehensive qPCR, western blot and enzyme activity analyses, we identified three key enzymes, CHS, ANS and 3GT, in purple-fleshed sweet potato storage roots in response to shading, which affect the root anthocyanin content by influencing the flavonoid metabolism pathway. This study provides a theoretical basis for revealing the regulation of anthocyanin synthesis in crops and a guidance for high-quality sweet potato cultivation and nutritional improvement using shade facilities.

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A metabolomic, transcriptomic profiling, and mineral nutrient metabolism study of the phytotoxicity mechanism of uranium

Cited by 68 publications

References 57 publications

Calcium-permeable cation channels are involved in uranium uptake in Arabidopsis thaliana

Calcium-permeable cation channels are involved in uranium uptake in Arabidopsis thaliana

Development of a metalloproteomic approach to analyse the response of Arabidopsis cells to uranium stress

Metabonomic Profiling Analyses Reveal ANS Upregulation to Enhance the Flavonoid Pathway of Purple-Fleshed Sweet Potato Storage Root in Response to Deep Shading

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