A Metabolomics Approach to Establish the Relationship between the Techno-Functional Properties and Metabolome of Indian Goat Yoghurt

Rehman, Hameedur; Saipriya, Kanchanpally; Singh, Ashish Kumar; Singh, Richa; Meena, Ganga Sahay; Khetra, Yogesh; Sharma, Heena

doi:10.3390/foods13060913

Cited by 3 publications

(1 citation statement)

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“…Functional yogurts with plant-sourced components from rice, pepper, and papaya reportedly have a low glycemic index, low fat content, and metabolism-related benefits ( 19–21 ). Meanwhile, yogurt fermented from unconventional media, such as goat milk, can provide functional flavor substances ( 22 ). Benefits related to intestinal barrier dysfunction, osteoporosis, and hyperglycemia have also been reported for yogurt containing different active substances ( 23 , 24 ).…”

Section: Introductionmentioning

confidence: 99%

Production and quality evaluation of a novel γ-aminobutyric acid-enriched yogurt

Zhu,

Hu,

Mei

2024

Front. Nutr.

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Objectiveγ-aminobutyric acid (GABA) is a neurotransmitter inhibitor that has beneficial effects on various health conditions such as hypertension, cognitive dysfunction, and anxiety. In this study, we investigated a novel yogurt naturally enriched with GABA using a Levilactobacillus brevis strain isolated in our laboratory; the specific optimum yogurt production conditions for this strain were determined.MethodsWe isolated an L. brevis strain and used it to produce yogurt naturally enriched with GABA. We explored the optimal conditions to enhance GABA yield, including fermentation temperature, inoculation amount, L-monosodium glutamate (L-MSG) concentration, fermentation time, and sucrose content. We also performed mixed fermentation with Streptococcus thermophilus and evaluated the quality of the yogurt.ResultsFollowing optimization (43°C, 8% inoculation amount, 1.5 g/L L-MSG, and 8% sucrose for 40 h of fermentation), the GABA yield of the yogurt increased by 2.2 times, reaching 75.3 mg/100 g. Mixed fermentation with S. thermophilus demonstrated favorable results, achieving a GABA yield akin to that found in some commercially available functional foods. Moreover, the viable microbe count in the GABA-enriched yogurt exceeded 1 × 108 cfu/mL, which is higher than that of commercial standards. The yogurt also exhibited a suitable water-holding capacity, viscosity, 3-week storage time, and favorable sensory test results.ConclusionThis study highlights the potential of naturally enriched GABA yogurt as a competitive commercial yogurt with beneficial health effects.

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Section: Introductionmentioning

confidence: 99%

Production and quality evaluation of a novel γ-aminobutyric acid-enriched yogurt

Zhu,

Hu,

Mei

2024

Front. Nutr.

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HRMAS-NMR-Based Metabolomics Approach to Discover Key Differences in Cow and Goat Milk Yoghurt Metabolomes

Kandasamy,

Park,

Bae

et al. 2024

Foods

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This study highlights the differences in the metabolomes of cow milk yoghurt (CY) and goat milk yoghurt (GY) using a nuclear magnetic resonance (NMR)-based metabolomic approach. The 1H HRMAS-NMR spectrum displayed 21 metabolites comprising organic acids, sugars, amino acids, amino acid derivatives and phospholipids. The orthogonal partial least squares discriminant analysis model clearly separated CY and GY groups, implying differences in metabolite composition. The corresponding Variable Importance in Projection (VIP) plot revealed that choline, sn-glycero-3-phosphocholine, O-phosphocholine, fucose, citrate, sucrose, glucose and lactose mainly contributed to the group separation (VIP > 1). Hierarchical cluster analysis further confirmed the metabolome similarities and differences between CY and GY. Additionally, 12 significantly differential metabolites (with a fold change > 1.5 and p-value < 0.05) were identified, with 1 downregulated and 11 upregulated. Pathway impact analysis revealed the correlation of significant metabolites with starch and sucrose metabolism, galactose metabolism, and the citrate cycle. Furthermore, receiver operating characteristic curve analysis identified eight metabolites (choline, sn-glycero-3-phosphocholine, fucose, O-phosphocholine, glucose, citrate, 2-oxoglutarate, lactose and sucrose) as candidate biomarkers. This study represents the first utilization of HRMAS-NMR to analyze the metabolomic profiles of yoghurt made from cow and goat milk. In conclusion, these findings provide preliminary information on how NMR-based metabolomics can discriminate the metabolomes of yoghurt prepared from the milk of two different animals, which may be valuable for authenticity and adulteration assessments.

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Microalgal Metabolomes and Recent Biotechnological Advances for Their Industrial Application

Saini,

Manchanda,

Chaudhary

et al. 2024

Microbiology Research

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In recent decades, microalgae have emerged as new sustainable resources for the production of various bioactive and biochemical compounds. These organisms are photosynthetic, use CO2 as their sole carbon source, and grow rapidly under different environmental conditions. This makes microalgae a promising source of feedstock for many bio-commodities. However, the economic viability for large-scale production through microalgal cells is limited to certain organisms. Recent advances in omics technologies, along with high-throughput approaches, have provided new insights for understanding microalgal metabolites. With the improvement in metabolomic technology, sensitivity for the detection of cellular metabolites has increased, and it has directly enhanced our understanding of cellular metabolism and the corresponding metabolic pathways. Moreover, these metabolic techniques, along with other omics technologies, help us to characterize the changes in the cellular functioning of the different cells under environmental conditions. Metabolomic research on microalgae explores new metabolites and the understanding of their biosynthesis at the metabolic level. In this review, we discuss how these metabolomics techniques are applied to microalgae to study their metabolic networks. Additionally, we also discuss how integrating other tools of systems biology can improve the productivity of microalgal cells, especially for industrially important commodities.

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A Metabolomics Approach to Establish the Relationship between the Techno-Functional Properties and Metabolome of Indian Goat Yoghurt

Cited by 3 publications

References 60 publications

Production and quality evaluation of a novel γ-aminobutyric acid-enriched yogurt

Production and quality evaluation of a novel γ-aminobutyric acid-enriched yogurt

HRMAS-NMR-Based Metabolomics Approach to Discover Key Differences in Cow and Goat Milk Yoghurt Metabolomes

Microalgal Metabolomes and Recent Biotechnological Advances for Their Industrial Application

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