A metabonomics-based renoprotective mechanism analysis of empagliflozin in obese mice

Chen, Xing; Chen, Shuchun; Ren, Qingjuan; Niu, Shu; Yue, Lin; Pan, Xiaoyu; Li, Zelin; Zhu, Ruiyi; Jia, Zhuoya; Chen, Xiaoyi; Zhen, Ruoxi; Ban, Jiangli

doi:10.1016/j.bbrc.2022.06.091

Cited by 3 publications

(2 citation statements)

References 33 publications

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“…Estrogen deficiency-induced weight gain has also been induced in mouse models of osteoporosis [ 50 ]. LPG 22:6 has been found to be significantly elevated in high-fat-induced obese mice [ 51 ]. Our study showed that LPG 22:6 was elevated in OVX mice, whereas nodakenin treatment decreased the level of LPG 22:6.…”

Section: Discussionmentioning

confidence: 99%

Nodakenin Ameliorates Ovariectomy-Induced Bone Loss by Regulating Gut Microbiota

Liu,

Chen,

Wang

et al. 2024

Molecules

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Disordered gut microbiota (GM) structure and function may contribute to osteoporosis (OP). Nodakenin has been shown to ameliorate osteoporosis; however, its anti-osteoporotic mechanism is unknown. This study aimed to further reveal the mechanism of the anti-osteoporotic action of nodakenin from the perspective of the microbiome and metabolome. An osteoporosis model was induced in mice through ovariectomy (OVX), with bone mass and microstructure assessed using μCT. Subsequently, ELISA and histologic examination were used to detect biochemical indicators of bone conversion and intestinal morphology. Using metabolomics and 16S rRNA sequencing, it was possible to determine the composition and abundance of the gut microbiota in feces. The results revealed that nodakenin treatment improved the bone microstructure and serum levels of bone turnover markers, and increased the intestinal mucosal integrity. 16S rRNA sequencing analysis revealed that nodakenin treatment decreased the relative abundance of Firmicutes and Patescibacteria, as well as the F/B ratio, and elevated the relative abundance of Bacteroidetes in OVX mice. In addition, nodakenin enhanced the relative abundance of Muribaculaceae and Allobaculum, among others, at the genus level. Moreover, metabolomics analysis revealed that nodakenin treatment significantly altered the changes in 113 metabolites, including calcitriol. A correlation analysis revealed substantial associations between various gut microbiota taxa and both the osteoporosis phenotype and metabolites. In summary, nodakenin treatment alleviated OVX-induced osteoporosis by modulating the gut microbiota and intestinal barrier.

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Section: Discussionmentioning

confidence: 99%

Nodakenin Ameliorates Ovariectomy-Induced Bone Loss by Regulating Gut Microbiota

Liu,

Chen,

Wang

et al. 2024

Molecules

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“…Lee's Index 13 On days 0, 7, 14, 30, and 45, the body weight and length of the rats were measured, and the Lee's indexes were calculated: Lee's index = (body weight × 1000)^(1/3) / body length.…”

Section: Measurement Index and Methods Samplingmentioning

confidence: 99%

Effect of Yak Meat to the Daily Ration of Scalded Rats for Wound Healing

Wang¹,

Feng²,

Tian³

et al. 2023

CCID

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Objective Treatment of burn wound healing involves infection, nutrition, psychology and rehabilitation, and proper nutritional support can promote wound healing, enhance immune function and reduce the incidence of complications. This study aimed to investigate the effects of feed containing yak meat on scalded rats’ body condition and wound healing. Methods Adopting a two-factor factorial design, the growth performance, food intake, body weight, and Lee’s index of rats were measured. The wound conditions of scalded rats with different feeds (basic, basic + yak meat, and basic + yellow beef) were observed at different periods, and their wounds’ hematoxylin and eosin (H&E) staining states were detected. The proliferating cell nuclear antigen (PCNA)-positive cells and apoptosis were analyzed to evaluate the effects of feed on the wound healing of scalded rats. Results The feed intake was the highest in the yellow beef feed group and the lowest in the yak meat feed group. The body weight was the highest in the yak meat feed group and the lowest in the yellow beef feed group. Furthermore, 45 days after scalding, the obesity index in the yak beef feed group was the closest to that of the rats before scalding. The wound recovery of the rats in the yak meat feed group was the best at 30 days, and the H&E staining results also proved that the recovery effect of the scalded rats in the yak meat feed group was better than other two groups. According to the results of PCNA and apoptosis, the yak meat feed group had lower positive cell rate and faster wound healing. Conclusion The rats in the yak meat feed group recovered better than those in the other groups, and the yak beef feed had the best effect on the wound healing of the scalded rats.

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SGLT2 inhibitor improves kidney function and morphology by regulating renal metabolism in mice with diabetic kidney disease

Yang,

Xiao,

Zhai

et al. 2024

Journal of Diabetes and its Complications

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A metabonomics-based renoprotective mechanism analysis of empagliflozin in obese mice

Cited by 3 publications

References 33 publications

Nodakenin Ameliorates Ovariectomy-Induced Bone Loss by Regulating Gut Microbiota

Nodakenin Ameliorates Ovariectomy-Induced Bone Loss by Regulating Gut Microbiota

Effect of Yak Meat to the Daily Ration of Scalded Rats for Wound Healing

SGLT2 inhibitor improves kidney function and morphology by regulating renal metabolism in mice with diabetic kidney disease

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