A metagenomic analysis of the modulatory effect of <i>Cyclocarya paliurus</i> flavonoids on the intestinal microbiome in a high‐fat diet‐induced obesity mouse model

Liu, Cheng; Chen, Yuhui; Zhang, Xin; Zheng, Xiaojie; Cao, Jinxuan; Wu, Zufang; Qin, Wenyan; Cheng, Kejun

doi:10.1002/jsfa.9622

Cited by 37 publications

(38 citation statements)

References 40 publications

(87 reference statements)

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“…Gut microbes can influence obesity by regulating metabolism, homoeostasis, appetite and energy balance, which together play crucial roles in obesity [ 25 ]. The structure, function and diversity of the gut microbiota among people with obesity are different from those of normal people [ 26 ]. Individuals with obesity usually show lower biodiversity and richness [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

Characteristics of gut microbiota in people with obesity

et al. 2021

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Background Obesity is the cause of cardiovascular diseases and other diseases, leading to increased medical costs, and causing a great burden to individuals, families and society. The prevalence of obesity is increasing and has become a global health problem. There is growing evidence that gut microbiota plays an important role in obesity. In this article, we revealed the differences in the gut microbiota between 21 people with obesity and 21 control subjects, and predicted the functional potential changes by 16S rRNA sequencing of the fecal bacteria of the subjects. Methods The raw sequencing data of 21 healthy Beijing volunteers was downloaded from Microbial Genome Database System. Microbial 16S rRNA genes of 21 adults with obesity were sequenced on an Illumina MiSeq instrument and analyzed by using bioinformatics and statistical methods. Results The diversity of gut microbiota in people with obesity decreased significantly. There were significant differences in gut microbiota between the Obesity and Control group at different levels. At the phylum level, Firmicutes, Bacteroidetes, Actinobacteria and Fusobacteria are significantly different between the Obesity and Control group. In people with obesity, the ratio of Firmicutes/Bacteroidetes decreased significantly. At the genus level, there were significant differences among the 16 major genera, of which four genera Prevotella, Megamonas, Fusobacterium and Blautia increased significantly in people with obesity, while the remaining 12 genera, Faecalibacterium, Lachnospiracea_incertae_sedis, Gemmiger and Clostridium XlVa, etc. decreased significantly. At the species level, nine species including Bacteroides uniformis and Prevotella copri had significant differences. Compared with the control group, subjects with obesity were abnormalities in 57 pathways, mainly in Carbohydrate metabolism and Lipid metabolism. Conclusions Overall, our study revealed differences in the gut microbiota between people with obesity and control subjects, providing novel target for the treatment of individuals with obesity.

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Section: Discussionmentioning

confidence: 99%

Characteristics of gut microbiota in people with obesity

et al. 2021

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“…Bacteroidetes species produce succinic acid, acetic acid, and in some cases propionic acid and plays an important role in protein metabolism. There is abundant evidence confirm that higher of Firmicutes/Bacteroidetes ratio of gut microbiota was found in obese patients ( De Bandt et al, 2011 ; Balfego et al, 2016 ; Louis et al, 2016 ) and HFD fed animals ( Huazano-Garcia et al, 2017 ; Wang H. et al, 2018 ; Cheng et al, 2019 ). Altered Firmicutes/Bacteroidetes ratio of gut microbiota in animals promotes increased ghrelin secretion correlated with MetS ( Perry et al, 2016 ).…”

Section: Discussionmentioning

confidence: 92%

High-Fat Diet Induced Gut Microbiota Alterations Associating With Ghrelin/Jak2/Stat3 Up-Regulation to Promote Benign Prostatic Hyperplasia Development

Liu

Yang

et al. 2021

Front. Cell Dev. Biol.

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The role of high-fat diet (HFD) induced gut microbiota alteration and Ghrelin as well as their correlation in benign prostatic hyperplasia (BPH) were explored in our study. The gut microbiota was analyzed by 16s rRNA sequencing. Ghrelin levels in serum, along with Ghrelin and Ghrelin receptor in prostate tissue of mice and patients with BPH were measured. The effect of Ghrelin on cell proliferation, apoptosis, and induction of BPH in mice was explored. Our results indicated that BPH mice have the highest ratio of Firmicutes and Bacteroidetes induced by HFD, as well as Ghrelin level in serum and prostate tissue was significantly increased compared with control. Elevated Ghrelin content in the serum and prostate tissue of BPH patients was also observed. Ghrelin promotes cell proliferation while inhibiting cell apoptosis of prostate cells. The effect of Ghrelin on enlargement of the prostate was found almost equivalent to that of testosterone propionate (TP) which may be attenuated by Ghrelin receptor antagonist YIL-781. Ghrelin could up-regulate Jak2/pJak2/Stat3/pStat3 expression in vitro and in vivo. Our results suggested that Gut microbiota may associate with Ghrelin which plays an important role in activation of Jak2/Stat3 in BPH development. Gut microbiota and Ghrelin might be pathogenic factors for BPH and could be used as a target for mediation.

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“…However, Cheng's FIGURE 10 | Funnel plot for publication bias in the meta-analysis. study showed that CP flavonoids can modulate gut microbiota to regulate certain metabolic processes, to the benefit of murine obesity models (60).…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, publication bias and selective reporting may also have affected the cumulative evidence. Flavonoids and triterpenoids are more likely to be the effective compounds in CPEE, and further studies performed on these phytochemicals have shown positive results (13,18,(59)(60)(61). Meanwhile, as the main components found in CPAE, polysaccharides have been previously investigated.…”

Section: Discussionmentioning

confidence: 99%

Effects of Cyclocarya paliurus Aqueous and Ethanol Extracts on Glucolipid Metabolism and the Underlying Mechanisms: A Meta-Analysis and Systematic Review

Liu

et al. 2020

Front. Nutr.

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Background and Aims:Cyclocarya paliurus (CP) has been used as an herbal tea to treat diabetes mellitus and obesity for hundreds of years. Previous research suggests that CP specifically restores glucolipid metabolic homeostasis, and the two most studied preparations are aqueous and ethanol extracts. In order to verify the effect of CP on glucolipid metabolism in animal models with metabolic syndrome, a meta-analysis was performed, and the active components and underlying mechanisms were systematically reviewed.Methods: Four databases: PubMed, Web of Science, Embase, and Cochrane Library were searched to identify potential literature. Data of blood glucose (BG) level, area under curve (AUC) of oral glucose tolerance test (OGTT), total cholesterol (TC), triglyceride (TG), high-density lipoprotein (HDL), and low-density lipoprotein (LDL) levels were extracted as indicators of the assessment of CP's effects. Follow-up analyses including subgroup analysis, meta-regressions, and publication bias were also conducted.Results: A total of 96 papers were identified from the databases and 11 papers including 31 data reports were involved in the meta-analysis. CP had a positive effect in down-regulating BG, AUC of OGTT, TC, TG, and LDL, and up-regulating HDL (P < 0.001, 95% confidence interval of standard mean difference did not incorporate the null value 0).Conclusion: CP showed definite activity of regulating glucolipid metabolism in animal models, and it exerted its function through multiple mechanisms including but not limited to: (1) improving insulin resistance; (2) protecting pancreatic β cells; (3) decreasing inflammatory infiltration; and (4) anti-oxidative stress.

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A metagenomic analysis of the modulatory effect of Cyclocarya paliurus flavonoids on the intestinal microbiome in a high‐fat diet‐induced obesity mouse model

Cited by 37 publications

References 40 publications

Characteristics of gut microbiota in people with obesity

Characteristics of gut microbiota in people with obesity

High-Fat Diet Induced Gut Microbiota Alterations Associating With Ghrelin/Jak2/Stat3 Up-Regulation to Promote Benign Prostatic Hyperplasia Development

Effects of Cyclocarya paliurus Aqueous and Ethanol Extracts on Glucolipid Metabolism and the Underlying Mechanisms: A Meta-Analysis and Systematic Review

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