A metagenomic survey identifies Tamdy orthonairovirus as well as divergent phlebo-, rhabdo-, chu- and flavi-like viruses in Anatolia, Turkey

Brinkmann, Annika; Dinçer, Ender; Polat, Ceylan; Hekimoğlu, Olcay; Hacıoğlu, Sabri; Földes, Katalin; Özkul, Aykut; Öktem, İbrahim Mehmet Ali; Nitsche, Andreas; Ergünay, Koray

doi:10.1016/j.ttbdis.2018.04.017

“…The variant of LTV described here was closely related to the variants found in Rhipicephalus microplus from China and Brazil, suggesting here again possible vector specificity [7,20]. However, although primarily described in Rhipicephalus microplus specimens, LTV has also been reported in Turkish Hyalomma marginatum and Rhipicephalus sanguineus ticks [53,56], and in Colombian Dermacentor nitens ticks (GenBank MK040531). In addition, we also detected LTV in Amblyomma variegatum specimens (10.4% to 11.9%).…”

Section: Discussionsupporting

confidence: 65%

RNA viruses of Amblyomma variegatum and Rhipicephalus microplus and Cattle Susceptibility in the French Antilles

Gondard¹,

Temmam²,

Devillers³

et al. 2019

Preprint

0

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Ticks transmit a wide variety of pathogens including bacteria, parasites and viruses. Over the last decade, numerous novel viruses have been described in arthropods, including ticks, and their characterization has provided new insights into RNA virus diversity and evolution. However, little is known about their ability to infect vertebrates. As very few studies have described the diversity of viruses present in ticks from the Caribbean, we implemented an RNA-sequencing approach on Amblyomma variegatum and Rhipicephalus microplus ticks collected from cattle in Guadeloupe and Martinique. Among the viral communities infecting Caribbean ticks, we selected four viruses belonging to the Chuviridae, Phenuiviridae and Flaviviridae families for further characterization and designing antibody screening tests. While viral prevalence in individual tick samples revealed high infection rates, suggesting a high level of exposure of Caribbean cattle to these viruses, no seropositive animals were detected. These results suggest that the Chuviridae- and Phenuiviridae-related viruses identified in the present study are more likely tick endosymbionts, raising the question of the epidemiological significance of their occurrence in ticks, especially regarding their possible impact on tick biology and vector capacity. The characterization of these viruses might open the door to new ways of preventing and controlling tick-borne diseases.

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“…The variant of LTV described here is closely related to the variants found in Rhipicephalus microplus from China and Brazil, again suggesting possible vector specificity [7,20]. However, although primarily described in Rhipicephalus microplus specimens, LTV has also been reported in Turkish Hyalomma marginatum and Rhipicephalus sanguineus ticks [53,56], and in Colombian Dermacentor nitens ticks (GenBank MK040531). In addition, we also detected LTV in Amblyomma variegatum specimens (10.4% to 11.9%).…”

Section: Discussionsupporting

confidence: 62%

“…KTV clustered with other Changping miviruses that have been identified in different hard tick species worldwide, such as the virus strains from Trinidad and Tobago, and Thailand in Rhipicephalus sanguineus ticks [15,22], Chinese strains in Dermacentor spp. or Haemaphysalis parva ticks [7], or Turkish strains in Haemaphysalis parva [53]. The relatively low level of genome sequence conservation between Changping mivirus isolates originating from different tick species reinforces the hypothesis of virus specialization and co-evolution with their respective tick hosts.…”

Section: Discussionmentioning

confidence: 67%

RNA Viruses of Amblyomma variegatum and Rhipicephalus microplus and Cattle Susceptibility in the French Antilles

Gondard

¹

,

Temmam

²

,

Devillers

³

et al. 2020

Viruses

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Ticks transmit a wide variety of pathogens including bacteria, parasites and viruses. Over the last decade, numerous novel viruses have been described in arthropods, including ticks, and their characterization has provided new insights into RNA virus diversity and evolution. However, little is known about their ability to infect vertebrates. As very few studies have described the diversity of viruses present in ticks from the Caribbean, we implemented an RNA-sequencing approach on Amblyomma variegatum and Rhipicephalus microplus ticks collected from cattle in Guadeloupe and Martinique. Among the viral communities infecting Caribbean ticks, we selected four viruses belonging to the Chuviridae, Phenuiviridae and Flaviviridae families for further characterization and designing antibody screening tests. While viral prevalence in individual tick samples revealed high infection rates, suggesting a high level of exposure of Caribbean cattle to these viruses, no seropositive animals were detected. These results suggest that the Chuviridae- and Phenuiviridae-related viruses identified in the present study are more likely tick endosymbionts, raising the question of the epidemiological significance of their occurrence in ticks, especially regarding their possible impact on tick biology and vector capacity. The characterization of these viruses might open the door to new ways of preventing and controlling tick-borne diseases.

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“…Human tick-borne infections have also been documented, caused by protozoans, nematodes, bacteria and viruses [9, 11]. We have recently reported the presence of several RNA viruses in ticks collected from various regions of Anatolia [12]. In the present study, we aimed to perform a cross-sectional screening by using next-generation sequencing (NGS) to characterize tick-associated bacteria and protozoans.…”

Section: Introductionmentioning

confidence: 99%

A cross-sectional screening by next-generation sequencing reveals Rickettsia, Coxiella, Francisella, Borrelia, Babesia, Theileria and Hemolivia species in ticks from Anatolia

Brinkmann

¹

,

Hekimoğlu

²

,

Dinçer

³

et al. 2019

Self Cite

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BackgroundTicks participate as arthropod vectors in the transmission of pathogenic microorganisms to humans. Several tick-borne infections have reemerged, along with newly described agents of unexplored pathogenicity. In an attempt to expand current information on tick-associated bacteria and protozoans, we performed a cross-sectional screening of ticks, using next-generation sequencing. Ticks seeking hosts and infesting domestic animals were collected in four provinces across the Aegean, Mediterranean and Central Anatolia regions of Turkey and analyzed by commonly used procedures and platforms.ResultsTwo hundred and eighty ticks comprising 10 species were evaluated in 40 pools. Contigs from tick-associated microorganisms were detected in 22 (55%) questing and 4 feeding (10%) tick pools, with multiple microorganisms identified in 12 pools. Rickettsia 16S ribosomal RNA gene, gltA, sca1 and ompA sequences were present in 7 pools (17.5%), comprising feeding Haemaphysalis parva and questing/hunting Rhipicephalus bursa, Rhipicephalus sanguineus (sensu lato) and Hyalomma marginatum specimens. A near-complete genome and conjugative plasmid of a Rickettsia hoogstraalii strain could be characterized in questing Ha. parva. Coxiella-like endosymbionts were identified in pools of questing (12/40) as well as feeding (4/40) ticks of the genera Rhipicephalus, Haemaphysalis and Hyalomma. Francisella-like endosymbionts were also detected in 22.5% (9/40) of the pools that comprise hunting Hyalomma ticks in 8 pools. Coxiella-like and Francisella-like endosymbionts formed phylogenetically distinct clusters associated with their tick hosts. Borrelia turcica was characterized in 5% (2/40) of the pools, comprising hunting Hyalomma aegyptium ticks. Co-infection of Coxiella-like endosymbiont and Babesia was noted in a questing R. sanguineus (s.l.) specimen. Furthermore, protozoan 18S rRNA gene sequences were detected in 4 pools of questing/hunting ticks (10%) and identified as Babesia ovis, Hemolivia mauritanica, Babesia and Theileria spp.ConclusionsOur metagenomic approach enabled identification of diverse pathogenic and non-pathogenic microorganisms in questing and feeding ticks in Anatolia.Electronic supplementary materialThe online version of this article (10.1186/s13071-018-3277-7) contains supplementary material, which is available to authorized users.

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A metagenomic survey identifies Tamdy orthonairovirus as well as divergent phlebo-, rhabdo-, chu- and flavi-like viruses in Anatolia, Turkey

Cited by 51 publications

References 38 publications

RNA viruses of Amblyomma variegatum and Rhipicephalus microplus and Cattle Susceptibility in the French Antilles

RNA viruses of Amblyomma variegatum and Rhipicephalus microplus and Cattle Susceptibility in the French Antilles

RNA Viruses of Amblyomma variegatum and Rhipicephalus microplus and Cattle Susceptibility in the French Antilles

A cross-sectional screening by next-generation sequencing reveals Rickettsia, Coxiella, Francisella, Borrelia, Babesia, Theileria and Hemolivia species in ticks from Anatolia

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