A metal-organic zeolitic framework with immobilized urease for use in a tapered optical fiber urea biosensor

Zhu, Guixian; Cheng, Lin; Qi, Ruogu; Zhang, Mizhen; Zhao, Jiahao; Zhu, Lianqing; Dong, Mingli

doi:10.1007/s00604-019-4026-0

Cited by 43 publications

(10 citation statements)

References 36 publications

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“…The amount captured or encapsulated by MOF was determined by decomposing the MOF in an acidic solution and then using UV–vis and Bradford assay for the quantification with the help of a standard curve generated with known concentrations of proteinase K (Figure S3B). Figure A shows that the efficiencies, on average, are 30.0, 46.6, and 75.2% for 0.5, 1, and 1.5 mg/mL concentration of proteinase K, respectively; note that the encapsulation efficiency of protein-concentration-dependent increase was similar to that of previous report …”

Section: Resultssupporting

confidence: 90%

“…Figure 4A shows that the efficiencies, on average, are 30.0, 46.6, and 75.2% for 0.5, 1, and 1.5 mg/mL concentration of proteinase K, respectively; note that the encapsulation efficiency of protein-concentrationdependent increase was similar to that of previous report. 34 Cell viability in the presence of bare proteinase K and proteinase K contained in MOF nanoparticles was probed by introducing them to MDA-MB-231 cells attached to a glass substrate. For this purpose, the proteinase K@MOF nanoparticles containing 0.15 mg of proteinase K were dispersed in (i) 1 mL of serum-free cell culture media and (ii) 0.15 mg of only bare proteinase K dispersed in the same media.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Interwoven MOF-Coated Janus Cells as a Novel Carrier of Toxic Proteins

Choi

Kim

2021

ACS Appl. Mater. Interfaces

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Two major issues in cell-mediated drug delivery systems (c-DDS) are the availability of free cell surfaces for the binding of the cells to the target or to their microenvironment and internalization of the cytotoxic drug. In this study, the Janus structure, MOF nanoparticles, and tannic acid (TA) are utilized to address these issues. Janus carrier cells coated with metal−organic frameworks (MOFs) are produced by asymmetrically immobilizing the nanoparticles of a MOF based on zinc with cytotoxic enzymes that are internally encapsulated on the surface of carrier cells. By maintaining the biological and structural features of regular living cells, the MOF-coated Janus cells developed in the present study preserve the intrinsic binding capacity of the cells to their microenvironment. Interconnected MOFs loaded onto the other face of the Janus cells cannot penetrate the cell. Therefore, the carrier cells are protected from the cytotoxic drug contained in MOFs. These MOF-Janus carrier cells are demonstrated to successfully eliminate three-dimensional (3D) tumor spheroids when a chemotherapeutic protein of proteinase K is released from the MOF nanoparticles in an acid environment. The ease with which the MOF-Janus carrier cells are prepared (in 15 min), and the ability to carry a variety of enzymes and even multiple ones should make the developed system attractive as a general platform for drug delivery in various applications, including combination therapy.

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Section: Resultssupporting

confidence: 90%

Section: ■ Results and Discussionmentioning

confidence: 99%

Interwoven MOF-Coated Janus Cells as a Novel Carrier of Toxic Proteins

Choi

Kim

2021

ACS Appl. Mater. Interfaces

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“…The main advantages of this optical urea biosensor for determination of urea were compact size, label free detection and very fast response. In addition, a micro fluidic chip has been integrated with the biosensor to conquer with the signal instability problem [49].…”

Section: Optical Urea Biosensormentioning

confidence: 99%

“…The current review illustrates the wide range of urea biosensor methods, including the use of a variety of materials like metallic particles, natural polymers, nanorange thin film, zero dimension quantum dots, sol‐gels, porous functionalised nanomaterials, zeolites and green metallic NPs [ 21 , 22 ]. This review also highlighted the latest and prospect trends in the utilisation of nanorange materials (metallic NPs, SWCNTs, MWCNTs, thin film nanostructure, functionalised nano‐entity, quantum dot, and bionanocomposites) for the manufacture of more perceptive and reliable urea sensors [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Recent advancements in urea biosensors for biomedical applications

Singh

Sharma

Singh

2021

IET Nanobiotechnology

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The quick progress in health care technology as a recurrent measurement of biochemical factors such as blood components leads to advance development and growth in biosensor technology necessary for effectual patient concern. The review wok of authors present a concise information and brief discussion on the development made in the progress of potentiometric, field effect transistor, graphene, electrochemical, optical, polymeric, nanoparticles and nanocomposites based urea biosensors in the past two decades. The work of authors is also centred on different procedures/methods for detection of urea by using amperometric, potentiometric, conductometric and optical processes, where graphene, polymer etc. are utilised as an immobilised material for the fabrication of biosensors. Further, a comparative revision has been accomplished on various procedures of urea analysis using different materials-based biosensors, and it discloses that electrochemical and potentiometric biosensor is the most promise one among all, in terms of rapid response time, extensive shelf life and resourceful design.This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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“…In order to enhance the biosensor performance and its suitability towards real time applications, variety of transducers and synthesized materials have been used. [1][2][3][4] Specifically, concerned to transducers in biosensors different modalities were found to be used such as electrochemical, optical, colorimetric, piezoelectric, and so forth. [5][6][7] The main function of the transducers is the conversion of the external stimulus into readable output signal based on its modality.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive polyaniline‐nickel oxide cladding modified with urease immobilized intrinsic optical fiber urea biosensor

Botewad

Gaikwad²,

Girhe³

et al. 2021

Polymers for Advanced Techs

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Herein, we report a polyaniline-nickel oxide (PANI-NiO) nanocomposite as an efficient immobilization matrix for development the optical fiber urea biosensor. Optical fiber sensing probe was developed by removing some portion of optical fiber at middle and modified with PANI-NiO matrix. After the modification of cladding removed portion, it was immobilized with enzyme urease via glutaraldehyde as a bi-functional cross-linking agent. The physicochemical and optical properties of the PANI-NiO matrix were explored by X-ray diffraction, scanning electron microscopy, ultravioletvisible, and Fourier transform infrared spectroscopic techniques. The characteristic features and performance of the developed sensor were evaluated via recording the output power and modal power distribution by means of a charge-coupled device camera. The developed urea biosensor exhibits a selective response towards urea concentrations in the linear range 1 nM-100 mM with a lower detection limit of 1 nM. Sensor recorded as a 40 days stability and response time $1 min. Thus, the obtained experimental results of the developed sensor promote its applicability with practical prospects in diverse field.

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A metal-organic zeolitic framework with immobilized urease for use in a tapered optical fiber urea biosensor

Cited by 43 publications

References 36 publications

Interwoven MOF-Coated Janus Cells as a Novel Carrier of Toxic Proteins

Interwoven MOF-Coated Janus Cells as a Novel Carrier of Toxic Proteins

Recent advancements in urea biosensors for biomedical applications

Ultrasensitive polyaniline‐nickel oxide cladding modified with urease immobilized intrinsic optical fiber urea biosensor

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