A Metastasis-Related lncRNA Signature Correlates With the Prognosis in Clear Cell Renal Cell Carcinoma

Abstract

To explore the role of metastasis-related long noncoding RNA (lncRNA) signature for predicting the prognosis of clear cell renal cell carcinoma (ccRCC) patients. Firstly, metastasis-associated genes were identified to establish a metastasis-related lncRNA signature by statistical analysis. Secondly, the ccRCC patients were grouped into high-risk or low-risk group according to the established signature, and the different pathways between the 2 groups were identified by gene set enrichment analysis (GSEA). Finally, investigations involving PCR, transwell migration and invasion assay were carried out to further confirm our findings. The metastasis-related lncRNA signature was successfully constructed according to 7-metastasis-related genes (ADAM12, CD44, IL6, TFPI2, TGF-β1, THBS2, TIMP3). The diagnostic efficacy and the clinically predictive capacity of the signature were evaluated. Most of the values of the area under the time‐dependent receiver‐operating characteristic (ROC) were greater than 0.70. The nomogram constructed by integrating clinical data and risk scores confirmed that the risk score calculated from our signature was a good prognosis predictor. GSEA analysis showed that some tumor-related pathways were enriched in the high-risk group, while metabolism-related pathways were enriched in the low-risk group. In carcinoma tissues, the SSR3-6, WISP1-2 were highly expressed, but the expression of UBAC2-6 was low there. Knocking down SSR3-6 decreased the ability of migration and invasion in ccRCC cells. In conclusion, we successfully constructed a metastasis-related lncRNA signature, which could accurately predict the survival and prognosis of ccRCC patients.

A novel oxidative-stress related lncRNA signature predicts the prognosis of clear cell renal cell carcinoma

“…It leads to high mortality owing to its aggressive and metastatic nature 20,21 . Recent studies have constructed signatures with m6, metastasis, glycolysis and immune-related lncRNAs to predict the prognosis of patients with ccRCC [22][23][24][25] . However, OS-related lncRNA signatures have not been constructed.…”

Identification of a combined lncRNA prognostic signature and knockdown of lncRNA MANCR to inhibit progression of clear cell renal cell carcinoma by bioinformatics analysis

“…Recently, Zeng et al constructed a 6-lncRNA-based risk score which could be used for ccRCC prognosis based on RNA-sequencing data ( 26 ). Dou et al also constructed 7-metastasis-related genes that correlated with the prognosis with the metastasis-related lncRNA signature in ccRCC ( 27 ). Su et al also showed that 3-key combined lncRNA signature was successfully used to predict poor prognosis in patients with ccRCC ( 28 ).…”

Necroptosis-related lncRNA in lung adenocarcinoma: A comprehensive analysis based on a prognosis model and a competing endogenous RNA network

“…Increasing evidence suggests that LUAD lncRNA plays an important role in prognosis and immunotherapeutic response. Several studies have developed lncRNA prognosis signatures, such as m6A-related ( Wang et al, 2021b ), ferroptosis-related ( Fei et al, 2021 ), pyroptosis-related ( Song et al, 2021 ), immune-related ( Qi et al, 2021 ), and metastasis-related lncRNA signatures ( Dou et al, 2021 ). However, NRLs are still being investigated, and their prognostic role in LUAD remains unclear.…”