A Method Based on Artificial Substrates to Monitor Hyporheic Biofilm Development

Claret, C.

doi:10.1002/iroh.19980830204

Cited by 21 publications

(9 citation statements)

References 33 publications

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“…Those on the back and sides were wiped away with Kimwipes (Kimberly-Clark Co.), and the tile was rinsed with Milli-Q water. Tiles were transferred to 100 ml Milli-Q water, and periphyton were removed by gentle sonication (20 kHz, 50 W, 3-4 min) according to the methods of Claret (1998). Half (50 ml) of the suspension was fixed with glutaraldehyde (final concentration, 1%) for enumeration of algae and bacteria.…”

Section: Methodsmentioning

confidence: 99%

Effects of solar ultraviolet radiation on the periphyton community in lotic systems: comparison of attached algae and bacteria during their development

Hodoki

2005

Hydrobiologia

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The effects of solar ultraviolet radiation (UVR) on the development of a periphyton community were studied in an outdoor artificial stream apparatus. Algal biomass, species composition, and bacterial cell density were measured under full sunlight and non-UVR (photosynthetically active radiation [PAR]-only) conditions. Attachment of algae was detected on days 6-9. Although the chlorophyll-a concentration under non-UVR conditions was 2-4 times that under full sunlight (PAR + UVR) throughout the experiment, neither net algal growth rate nor species composition differed significantly between the two light conditions. The relative carotenoid pigment contents of attached algae in the PAR + UVR condition were 1.1-1.3 times those in the non-UVR condition. Rates of increase of bacterial cell densities under the PAR + UVR condition were depressed by solar UVR for the first few days, although there were no apparent differences in the rates of increase between the light conditions later in the experiment. The small effect of UVR on the development of this periphyton community may be attributable to low UV flux at this study site and to the experimental conditions under which the algae were kept: a high physiological state with high nutrient conditions. Attached bacteria and algae that colonize substrata first are likely to be sensitive to solar UVR, and the negative effects of UVR are mitigated by the development of a periphyton community.

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Section: Methodsmentioning

confidence: 99%

Effects of solar ultraviolet radiation on the periphyton community in lotic systems: comparison of attached algae and bacteria during their development

Hodoki

2005

Hydrobiologia

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“…Bacterial abundance was measured by epifluorescence microscopy count after DAPI staining (4.6 diamino-2 phenylindol) (Porter & Feig, 1980). Samples were prepared according to Claret (1998). Pictures of DAPI stained bacteria were taken by epifluorescence microscopy (Nikon eclipse TE 300, Osram, HBO 103 w) and analyzed using the Lucia 4.6 image analysis system (Laboratory imaging Ltd).…”

Section: Chemical and Biological Analysesmentioning

confidence: 99%

Do parafluvial zones have an impact in regulating river pollution? Spatial and temporal dynamics of nutrients, carbon, and bacteria in a large gravel bar of the Doubs River (France)

Déforêt

Marmonier²,

Rieffel³

et al. 2008

Hydrobiologia

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International audienceThe changes in both physical and chemical properties of interstitial water were investigated within a large gravel bar to determine if the parafluvial zone was a hotspot for nutrient transformations in a lowland eutrophic river, the Doubs (Eastern France). Interstitial water was sampled in 30 piezometers along five transects across the gravel bar, surface water was sampled in main and chute channels. Five campaigns of sampling were performed among summer and winter. In both interstitial and surface samples, water chemistry (electrical conductivity, pH, chloride, dissolved oxygen concentrations-DO) and nutrient concentrations (nitrate-NO3 (-), ammonium-NH4 (+), soluble reactive phosphorus-SRP, dissolved organic carbon-DOC) were measured. Moreover, temperature, water level, sediment grain size distribution and total bacterial abundance were assessed along a flowpath through a lateral gravel bar of the Doubs River. Measurements of water table elevation in the bar, main and chute channels indicated that the parafluvial flowpath was perpendicular to the main channel. Very low changes in chloride concentration and electrical conductivity showed minor groundwater input along the flowpath. The parafluvial zone was 0.9 m thick under the mean piezometric level, hydraulic gradients along the flowpath were 0.3%, and the discharge of interstitial water through the bar calculated at low flow was 40.6 m(3) day(-1). Most changes in interstitial nutrients occurred during the warm season, suggesting that biotic mechanisms occurred. Along the flowpath, DO, DOC, and bacterial abundance declined, while phosphate increased. Temporal trends of nitrate were less clear, exhibiting a slight increase during spring (organic matter biodegradation) and a decrease during summer (denitrification). Using the parafluvial discharge and nutrient concentrations of water infiltrating into and seeping from the gravel bar, the retention capacity of the bar was 171 gC day(-1) for DOC and 48.3 gN day(-1) for nitrate. Phosphate production ranged from 0.65 to 2.3 gP day(-1). These values were low compared to the nutrient fluxes in the river Doubs, suggesting a minimal local impact of the parafluvial zone in regulating of river pollution

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“…The lack of between-site differences could be due to similar physical and chemical conditions at both sites, at least on the sediment and rock surfaces. The number of cells recorded on the artificial substrates are within the range given in related studies (BÄRLOCHER and MUDORCH, 1989;CLARET, 1998).…”

Section: Discussionmentioning

confidence: 90%

A Study of Biofilm in a Second Order Tropical Stream, Njoro River, Kenya: First Results

Muia¹,

Bretschko²,

Herndl³

2003

International Review of Hydrobiology

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Between January and December 1999 the extent of biofilm development has been studied at two sites (muddy sediment site [A] and sandy sediment site [B]) in Njoro River, Kenya. Grain size, organic matter and bacterial abundance were determined at different depth layers of the sediment. Particulate carbohydrate concentrations were measured as an indicator of biofilm development in the sediment. Additionally, the development of the biofilm was investigated by placing glass slides at rock and sediment surfaces and in different sediment depth layers. Our first results for sediment samples collected in January 1999 and growth on slides exposed for 28 days in March 1999 is presented. Grain size analysis revealed that sediment particles <1 mm contributed between 82 to 91% at site A and 45 to 67% at site B depending on the sediment layer. At site A, total organic matter (% weight loss on ignition) and total organic carbon (TOC) were more or less constant with depth while both parameters significantly decreased with depth at site B. Total organic nitrogen (TON) was detectable at all depth layers at site A but only in the top 40 mm layer at site B. Bacterial numbers were highest in the top 40 mm layer at both sites. Bacterial abundance of 3.95 ± 5.96 × 10 8 and 4.10 ± 3.88 × 10 8 cells g -1 dry sediment were recorded at site A and B, respectively. For both sites, highest concentrations of colloidal carbohydrates occurred in the top 40 mm layer, but concentrations were more or less constant below. Bacterial abundance on glass slides was higher on slides placed on rock surfaces than on fine sediment surfaces or within fine sediments.

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A Method Based on Artificial Substrates to Monitor Hyporheic Biofilm Development

Cited by 21 publications

References 33 publications

Effects of solar ultraviolet radiation on the periphyton community in lotic systems: comparison of attached algae and bacteria during their development

Effects of solar ultraviolet radiation on the periphyton community in lotic systems: comparison of attached algae and bacteria during their development

Do parafluvial zones have an impact in regulating river pollution? Spatial and temporal dynamics of nutrients, carbon, and bacteria in a large gravel bar of the Doubs River (France)

A Study of Biofilm in a Second Order Tropical Stream, Njoro River, Kenya: First Results

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