1977
DOI: 10.1111/j.1365-2621.1977.tb08427.x
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A Method for Determination of Tannins in Foods by Means of Immobilized Protein

Abstract: Immobilized bovine serum albumin (BSA) binds tannins selectively at pH 4. Monomeric polyphenolic substances such as gallic acid, chlorogenie acid and catechol do not interact with the protein under these conditions. The protein-tannin complexes are dissociated by organic solvents such as methanol or dimethylformamide and the released tannins may be recovered in pure form. These principles have been utilized in the design of an analytical method which utilizes small columns of Sepharose-BSA to achieve separatio… Show more

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Cited by 152 publications
(79 citation statements)
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“…The total phenolic content was determined by the FolinCiocalteu method, using gallic acid as the standard (Hoff and Singleton 1977;Muhammad 2006). Two grams of frozen tomato sample was homogenized in 100% methanol (20 ml).…”
Section: Total Phenolic Contentmentioning
confidence: 99%
“…The total phenolic content was determined by the FolinCiocalteu method, using gallic acid as the standard (Hoff and Singleton 1977;Muhammad 2006). Two grams of frozen tomato sample was homogenized in 100% methanol (20 ml).…”
Section: Total Phenolic Contentmentioning
confidence: 99%
“…Polyphenols were measured with the Folin-Ciocalteau reagent, according to the method of Hoff and Singleton [1966]. Gallnut tannic acid (Sigma Chemical Co., St. Louis, Mo., USA) was used as a standard.…”
Section: Chemical Assaysmentioning
confidence: 99%
“…Total phenols concentration was measured according to Hoff and Singleton (1977). Fifty lL of the methanol extract was mixed with 100 lL Folin-Ciocalteu reagent, 850 lL of methanol and allowed to stand for 5 min at ambient temperature.…”
Section: Estimation Of Total Phenolsmentioning
confidence: 99%