A method for determining concentration profiles at the soil-root interface by thin slicing rhizospheric soil

Kuchenbuch, R.; Jungk, A.

doi:10.1007/bf02197944

Cited by 159 publications

(73 citation statements)

References 9 publications

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“…The rhizobox was first introduced by Kuchenbuch and Jungk (1982) and Youssef and Chino (1988) and consists of a soil culture system where the root is separated from the soil by a porous membrane with a mesh facilitating the exchange of solutes but preventing roots from penetrating the soil. This culture system allows the precise study of RSA over a long period of time through a transparent Plexiglas plate as well as molecular and biochemical measurements on a clean (devoid of soil) and integral (including the finest roots and root hairs) root system.…”

Section: Choice Of the Experimental Proceduresmentioning

confidence: 99%

Water Deficit Enhances C Export to the Roots in Arabidopsis thaliana Plants with Contribution of Sucrose Transporters in Both Shoot and Roots

et al. 2016

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Section: Choice Of the Experimental Proceduresmentioning

confidence: 99%

Water Deficit Enhances C Export to the Roots in Arabidopsis thaliana Plants with Contribution of Sucrose Transporters in Both Shoot and Roots

et al. 2016

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“…As discussed in the section on external PUE above, under field conditions, the upper soil layer is often the richest in P. Another example is the rhizosphere study container technique (RSC) of Zoysa et al (1997Zoysa et al ( , 1999 consisting of 2 or 3 soil compartments separated by a 24 lm pore-diameter polyester mesh that retained the roots as a planar mat. At harvesting time, soils from each compartment can be sliced using freezing by liquid N2 (Kuchenbuch and Jungk 1982). The slices from the compartment with the plant can be used to measure the root surface area, in particular the mat at the polyester mesh.…”

Section: Soil (Solid)-based Systemsmentioning

confidence: 99%

Improving phosphorus use efficiency in agriculture: opportunities for breeding

Wiel¹,

Linden²,

Scholten³

2015

Euphytica

195

115

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Phosphorus (P) is often an important limiting factor for crop yields, but rock phosphate as fertilizer is a non-renewable resource and expected to become scarce in the future. High P input levels in agriculture have led to environmental problems. One of the ways to tackle these issues simultaneously is improving phosphorus use efficiency (PUE) of the crops through breeding. In this review, we describe plant architectural and physiological traits important for PUE. Subsequently, we discuss efficient methods of screening for PUE traits. We address targeted cultivation methods, including solid and hydroponic systems, as well as testing methods, such as image analysis systems, and biomass and photosynthesis measurements. Genetic variation for PUE traits has been assessed in many crops, and genetics of PUE has been studied by quantitative trait loci (QTL) analyses and genome-wide association study. A number of genes involved in the plant's response to low P have been characterized. These genes include transcription factors, and genes involved in signal transduction, hormonal pathways, sugar signalling, P saving metabolic pathways, and in P scavenging, including transporters and metabolites and/or ATP-ases mobilizing P in the soil. In addition, the role of microorganisms promoting PUE of plants, particularly arbuscular mycorrhizal fungi is discussed. An overview is given of methods for selecting for optimal combinations of plant and fungal genotypes, and their genetics, incl. QTLs and genes involved. In conclusion, significant progress has been made in selecting for traits for PUE, developing systems for the difficult but highly relevant root phenotyping, and in identifying QTLs and genes involved.

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“…The nitrate concentration gradients at the soil-LDH interface were determined with a modified method according to Kuchenbuch and Jungk (1982). Two PVC cylinders (5 cm height and 6.8 cm diameter) were put together (one on top of the other) and stabilized with adhesive tape.…”

Section: Determination Of the Soil Nitrate Gradients From The Ldh Surmentioning

confidence: 99%

Capacity, selectivity, and reversibility for nitrate exchange of a layered double‐hydroxide (LDH) mineral in simulated soil solutions and in soil

Torres‐Dorante

Lammel

Kuhlmann

et al. 2008

Z. Pflanzenernähr. Bodenk.

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The leaching of nitrate is an important way of N losses from agricultural soils in humid regions. Nitrate leaching is difficult to control as most soils under crop production do not have anionexchange properties, and nitrate remains mobile in the solution. The present work evaluated the potential use of a synthetic layered double-hydroxide (LDH) mineral as a nitrate exchanger in soil. The LDH used was a chloride form of a magnesium-aluminum layered double hydroxide with the formula: [Mg 2+ 0.82 Al 3+ 0.18 (OH) 2 ] 0.18+ [(Cl -) 0.18 0.5(H 2 O)] 0.18-. Experiments were carried out in aqueous solutions as well as in soil with the following objectives: (1) to characterize the nitrate adsorption capacity on the LDH, (2) to study its selectivity for nitrate adsorption in solution, (3) to evaluate the reversibility for nitrate exchange, and (4) to study the nitrate adsorption capacity and nitrate diffusion towards the LDH in soil. In aqueous solutions, the nitrate adsorption capacity was determined to be 1.54 mol c kg -1 LDH at nitrate concentrations typically encountered in soils under crop production. In solution, the LDH showed a high selectivity for nitrate adsorption compared to other anions. The LDH exchanged nitrate reversibly without a measurable reduction of the exchange capacity for at least 15 times. Under soil conditions, the extension of the nitrate depletion zone from the LDH surface reached up to about 8 cm within one week, confirming its high nitrate adsorption capacity also in soil. The LDH mineral evaluated in this work has the potential to be used as a nitrate exchanger in crop production, aiming to control the movement of nitrate in soil and thereby reducing the risks of nitrate leaching.

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A method for determining concentration profiles at the soil-root interface by thin slicing rhizospheric soil

Cited by 159 publications

References 9 publications

Water Deficit Enhances C Export to the Roots in Arabidopsis thaliana Plants with Contribution of Sucrose Transporters in Both Shoot and Roots

Water Deficit Enhances C Export to the Roots in Arabidopsis thaliana Plants with Contribution of Sucrose Transporters in Both Shoot and Roots

Improving phosphorus use efficiency in agriculture: opportunities for breeding

Capacity, selectivity, and reversibility for nitrate exchange of a layered double‐hydroxide (LDH) mineral in simulated soil solutions and in soil

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