2017
DOI: 10.12665/j16oa.cheever
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A Method for Differentiating Fetal Bovine Serum from Newborn Calf Serum

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Cited by 10 publications
(15 citation statements)
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“…Notably, the content of Mg 2+ in DMEM is about 0.81 mmol/L according to the information from the manufacturer, and 0.26 mmol/ L in FBS as reported. 17 Continually, the culture was maintained in a normoxic condition (5% CO 2 , 21% O 2 , and 74% N 2 ) relative to a hypoxic environment (5% CO 2 , 3% O 2 , and 92% N 2 ) at 37°C. To block phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) and nuclear factor κB (NF-κB), LY294002 (10 µM) and BAY 11-7082 (10 µM) (both from Beyotime Biotechnology, Shanghai, China) were incubated with HT22 cells for 12 hours.…”
Section: Cell Culture and Treatmentmentioning
confidence: 99%
“…Notably, the content of Mg 2+ in DMEM is about 0.81 mmol/L according to the information from the manufacturer, and 0.26 mmol/ L in FBS as reported. 17 Continually, the culture was maintained in a normoxic condition (5% CO 2 , 21% O 2 , and 74% N 2 ) relative to a hypoxic environment (5% CO 2 , 3% O 2 , and 92% N 2 ) at 37°C. To block phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) and nuclear factor κB (NF-κB), LY294002 (10 µM) and BAY 11-7082 (10 µM) (both from Beyotime Biotechnology, Shanghai, China) were incubated with HT22 cells for 12 hours.…”
Section: Cell Culture and Treatmentmentioning
confidence: 99%
“…Regulatory authorities clarified that “the use of adequately controlled bovine sera is allowed.” Reinhardt and colleagues specify that FBS must be controlled according to the European Pharmacopoeia , 7th ed, Monograph 01/2008:2262 Bovine Serum, and manufacturing shall involve virus testing and inactivation (e.g., irradiation at a dose higher than 30 kGy). The risk of transmitting animal spongiform encephalopathy agents, in particular, has prompted the search for alternative supplements from nonanimal origin …”
Section: Hpl As An Alternative To Fbsmentioning
confidence: 99%
“…There is an increasing market for FBS that is dependent in large part on the beef processing industry . Increasing demand from life science and pharmaceutical users, restricted supply due to climate changes, and reduced cattle inventories have impacted costs and have led to questionable practices in production.…”
Section: Hpl As An Alternative To Fbsmentioning
confidence: 99%
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“…27 The solvation parameters used for the PP-LFERs 28 and other parameters needed for the model predictions were obtained from published literature values (Table S4). 22,45,46 For comparison, the free concentration of PCBs in the cell culture system was calculated from the measured PCBs levels in medium ( ) and the sorptive capacities of the biological components in medium (for more details, see the Supporting Information), i.e.,…”
Section: Introductionmentioning
confidence: 99%