A Method for Distinguishing 1-Acyl from 2-Acyl Lysophosphatidylcholines Generated in Biological Systems

Florin‐Christensen, J.; Narváez-Vásquez, Javier; Florín-Christensen, Mónica; Ryan, Clarence A.

doi:10.1006/abio.1999.4322

Cited by 14 publications

(10 citation statements)

References 23 publications

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“…Recently, using a novel method for distinguishing 1-acyl from 2-acyl LPC, we showed that the LPC found in bovine erythrocyte extracts is predominantly of the type 1-acyl, i.e., the type generated by Plase A 2 (33). Furthermore, we found that no changes in lipid composition occurred in erythrocytes incubated in either heparinized plasma or in TBS-GCM buffer at 37°C for up to 48 h (data not shown).…”

Section: Resultsmentioning

confidence: 78%

A unique phospholipid organization in bovine erythrocyte membranes

Florin‐Christensen

Suárez

Florín-Christensen

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

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Ruminant erythrocytes are remarkable for their choline-phospholipid anomalies; namely, low or absent phosphatidylcholine (PC) along with high sphingomyelin levels. Here, we report another anomaly in bovine erythrocytes that affects aminophospholipids: phosphatidylethanolamine (PE) shows an extreme asymmetry, with only 2% of the total present in the outer leaflet. Furthermore, we found that phospholipase A2, an enzyme located on the external surface of the erythrocytes, shows higher activity against PC than against PE. In addition, we observed that acylation of PE is by far the most important biosynthetic event in this system. We propose that deacylation of PE and PC by phospholipase A2 to generate lysocompounds, followed by selective reacylation of lyso-PE in the inner leaflet, can account for the compositional and architectural peculiarities of bovine erythrocyte membranes.

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Section: Resultsmentioning

confidence: 78%

A unique phospholipid organization in bovine erythrocyte membranes

Florin‐Christensen

Suárez

Florín-Christensen

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

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“…Most of the papers and our previous methods of positional analysis of PC are based on two different enzymatic reactions (Adlecreutz & Wehtje, 2004;Chojnacka et al, 2009;Florin-Christensen et al, 1999;Gladkowski et al, 2009), which increase their complexity. Some papers also present one-enzymatic reaction methods of determination of FAs composition in PC (Cossignani et al, 1994).…”

Section: Resultsmentioning

confidence: 99%

“…These compounds were chemically hydrolyzed and the released FAs were esterified and analyzed by GC (Adlecreutz & Wehtje, 2004). A two-enzymes system using PLA 2 from bee venom and PLA 1 from T. thermophila was proposed by Florin-Christensen, Narvaez-Vasquez, Florin-Christensen, and Ryan (1999). Although the methods based on enzymatic hydrolysis of PLs are the most common procedures used in positional analysis of PC some other were also proposed.…”

Section: Introductionmentioning

confidence: 99%

A simple method for positional analysis of phosphatidylcholine

et al. 2012

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“…A 0.2-mL aliquot of the phospholipid fraction obtained by SPE was subjected to phospholipase A 2 catalyzed hydrolysis using the method described by Florin-Christensen et al [20]. Reaction products were separated by TLC on silica gel G plates with chloroform/methanol/acetic acid/ water (65:35:4:2, v/v/v/v).…”

Section: Positional Analysis Of Phospholipidsmentioning

confidence: 99%

Fatty Acid Composition of Irvingia gabonensis and Treculia africana Seed Lipids and Phospholipids

Ifeduba

Sabir

Akoh

2013

J Americ Oil Chem Soc

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Lipid compositions of two non‐conventional oilseeds (Irvingia gabonensis and Treculia africana) were studied. Total lipids were extracted by the Folch method and phospholipids were isolated by solid phase extraction. Fatty acid compositions of total lipids and phospholipids were determined by gas chromatography. Phospholipid classes and tocopherols were quantified by HPLC. The major fatty acids in I. gabonensis seed total lipids were myristic (41.4–48.9 %) and lauric (39.8–46.8 %) while those in T. africana seed were linoleic (29.1–31.4 %) and oleic (22.9–25.9 %). The principal fatty acid at the sn‐2 position of I. gabonensis seed triacylglycerols was myristic (49.5 %) while that of T. africana was linoleic (50.6 %). Phospholipid content of crude T. africana seed oil was 3.3 % and that of I. gabonensis was 0.1–0.3 %. The composition and distribution of saturated fatty acids in I. gabonensis seed lipids suggest that it may contribute to cardiovascular disease risk factors among the Igbo people in Nigeria that use the seed as food ingredient.

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A Method for Distinguishing 1-Acyl from 2-Acyl Lysophosphatidylcholines Generated in Biological Systems

Cited by 14 publications

References 23 publications

A unique phospholipid organization in bovine erythrocyte membranes

A unique phospholipid organization in bovine erythrocyte membranes

A simple method for positional analysis of phosphatidylcholine

Fatty Acid Composition of Irvingia gabonensis and Treculia africana Seed Lipids and Phospholipids

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