A Method for Improving the Efficiency of Therapy for Melanoma

Abstract

A method for photodynamic therapy was developed in experiments on the model of melanoma B16. The photosensitizer is injected by two doses, while laser exposure is carried out during coincidence of its maximum accumulation phases in tumor vessels and cells. This method increased the percentage of animals with complete regression of the tumor, decreased the coefficient of absolute tumor increase in animals with progressive tumor growth, increased survival of mice, and significantly decreased melanoma metastasizing to the lungs in comparison with untreated animals and even with the standard photodynamic therapy.

The Menace of Melanoma: A Photodynamic Approach to Adjunctive Cancer Therapy

“…Chloroaluminum phthalocyanine (CIAIPc) [81] in vitro A375 melanoma cells 5,15-Diarylporphyrins (1-5) and Photofrin [82] in vivo B16 melanoma tumours on mice 2 doses of photosensitizer [83] in vitro B16 mouse melanoma cells chlordiazepoxide (CDZ) [84] in [88] in vitro UCT Mel-1 and A375 human melanoma cells hypericin [89] in vitro G361 human melanoma cells chloroaluminum phthalocyanine (ClAlPc) and ultrasound [90] in vitro A375 human melanoma cells 5-aminolevulinic acid (ALA) [91] in [107] in vivo choroidal melanomas in 46 New Zealand albino rabbit eyes hematoporphyrin monomethyl ether [108] in vitro G361 human melanoma cells 3 porphyrin sensitizers (TPPS(4), ZnTPPS [4] and PdTPPS(4)) [109] in vitro YUSAC2/T34A-C4 human melanoma cell line porfimer sodium [110] in vivo subcutaneous B16BL6 melanoma-bearing C57BL/6 mice BPD, ce6, Photofrin, and mTHPC and gamma-inulin [111] in vitro A375 human melanoma cells acridine orange [112] in vitro Cloudman S91/I3 mouse melanoma cells photofrin II (PfII-porfirmer sodium), verteporfin, and merocyanine 540 (MC540) [113] in vivo [123] in vitro B16 mouse melanoma cells 5-aminolevulinic acid [5-ALA) ester derivatives [124] in vivo B-16 melanoma-bearing C57BL/6 mice metal-free sulfonated phthalocyanine (H(2)PcS(2.4)) [125] in vitro A375 human melanoma cells alpha-methylene-gamma-butyrolactone-psoralen heterodimer 2 [126] in vitro B16 mouse melanoma cells 5-aminolevulinic acid (ALA) [127] in vivo C57BL6 mice bearing a subcutaneously injected B16F10 melanoma silkworm excreta (SPbalpha) porfirmer sodium [128] in vitro M3Dau human melanoma cells silicon-phthalocyanines (SiPc) and chloroaluminium Pc (ClAlPc), [129] in vitro Me45 human melanoma cells meso-tetra-4-N-methylpyridyl-porphyrin iodide and…”