A Method for Measuring the Three-Dimensional Refractive-Index Distribution of Single Cells Using Proximal Two-Beam Optical Tweezers and a Phase-Shifting Mach—Zehnder Interferometer

Yasokawa, Toshiki; Ishimaru, Ichiro; Kondo, Masahiro; Kuriyama, Shigeki; Masaki, Tsutomu; Takegawa, Kaoru; Tanaka, Naotaka

doi:10.1007/s10043-007-0161-7

Cited by 19 publications

(8 citation statements)

References 6 publications

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“…In order to acquire quantitative information of the observed sample without labeling, interferometric phase microscopy (IPM) can be used [13][14][15]. Previous works combined optical tweezers and IPM for cells manipulation [16][17][18][19][20][21], however none of them integrated IPM with array of optical traps created by holography methods for TPM of live cells in suspension.…”

Section: Introductionmentioning

confidence: 99%

Tomographic phase microscopy using optical tweezers

et al. 2015

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We review our technique for tomographic phase microscopy with optical tweezers [1]. This tomographic phase microscopy approach enables full 3-D refractive-index reconstruction. Tomographic phase microscopy measures quantitatively the 3-D distribution of refractive-index in biological cells. We integrated our external interferometric module with holographic optical tweezers for obtaining quantitative phase maps of biological samples from a wide range of angles. The close-tocommon-path, off-axis interferometric system enables a full-rotation tomographic acquisition of a single cell using holographic optical tweezers for trapping and manipulating with a desired array of traps, while acquiring phase information of a single cell from all different angles and maintaining the native surrounding medium. We experimentally demonstrated two reconstruction algorithms: the filtered back-projection method and the Fourier diffraction method for 3-D refractive index imaging of yeast cells.

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Section: Introductionmentioning

confidence: 99%

Tomographic phase microscopy using optical tweezers

et al. 2015

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“…A large variety of biomedical applications (among others, the area of drug discovery) may benefit from an alternative approach to scattering detection by flow cytometry (complex and expensive method) and to fluorescence measurements (characterized by a grade of cell toxicity) [17]. Several methods have been proposed to measure the cell RI, based on microscopy techniques [7,18,19,20,21,22,23,24], on optical trapping [25,26], or on hydro-mechanic holders [4]. Microscopy approaches require bulky imaging systems, complex analytical algorithms and allow measurements on living cells adherent to a slide or on histological slices [14].…”

Section: Introductionmentioning

confidence: 99%

Low-Coherence Reflectometry for Refractive Index Measurements of Cells in Micro-Capillaries

Carpignano

Rigamonti

Mazzini

et al. 2016

Sensors

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The refractive index of cells provides insights into their composition, organization and function. Moreover, a good knowledge of the cell refractive index would allow an improvement of optical cytometric and diagnostic systems. Although interferometric techniques undoubtedly represent a good solution for quantifying optical path variation, obtaining the refractive index of a population of cells non-invasively remains challenging because of the variability in the geometrical thickness of the sample. In this paper, we demonstrate the use of infrared low-coherence reflectometry for non-invasively quantifying the average refractive index of cell populations gently confined in rectangular glass micro-capillaries. A suspension of human red blood cells in plasma is tested as a reference. As a use example, we apply this technique to estimate the average refractive index of cell populations belonging to epithelial and hematological families.

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“…Three-dimensional reconstruction is applied to reflect inner refractive index distribution of these biological samples. Many techniques for 3-D reconstruction have been proposed, including rotating biological samples in micropipette [11] or optical systems [12] for scanning, designing LED linear array for large angle scanning [13], applying optical tweezers to control the samples for whole rotation degrees [14], and using synthetic aperture with Hyugens' wavelet principle for 3-D reconstruction [15], etc. All these methods could obtain 3-D refractive index of cells with different methods.…”

Section: Introductionmentioning

confidence: 99%

Quantitative interferometric microscopy: tool for phase imaging from 2D to 3D

Wang

Lai

et al. 2012

SPIE Proceedings

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Phase distribution detection of cells and tissues is concerned since it is an important auxiliary method for observing biological samples. Here, in this paper, we have proposed phase retrieval algorithms dealing with microscopic interferograms in order to solve two-dimensional phase distribution. Based on phase distributions solved by phase retrieval algorithms, three-dimensional refractive index distribution of biological sample is reconstructed which could reflect inner structure of the cell. We believe these methods could be powerful tools in biological and medical fields.

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A Method for Measuring the Three-Dimensional Refractive-Index Distribution of Single Cells Using Proximal Two-Beam Optical Tweezers and a Phase-Shifting Mach—Zehnder Interferometer

Cited by 19 publications

References 6 publications

Tomographic phase microscopy using optical tweezers

Tomographic phase microscopy using optical tweezers

Low-Coherence Reflectometry for Refractive Index Measurements of Cells in Micro-Capillaries

Quantitative interferometric microscopy: tool for phase imaging from 2D to 3D

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