A Method for Obtaining Ribbons of Serial Sections of Plastic Embedded Specimens

Henry, Eric C.

doi:10.3109/10520297709116748

Cited by 43 publications

(27 citation statements)

References 2 publications

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“…For sectioning the larvae were dehydrated in an ethanol series and embedded in low viscosity resin (Spurr 1969). For light microscopy ribboned serial sections (1-2 µm thick) were prepared with "Ralph" glass knives and contact cement ("Pattex-compact") at the lower cutting edge (Henry 1977). The sections were stained by methylene-blue -azure II (Richardson et al 1960).…”

Section: Culture and Breeding Of Patellamentioning

confidence: 99%

Development of the musculature in the limpet Patella (Mollusca, Patellogastropoda)

Wanninger¹,

Ruthensteiner²,

Lobenwein

et al. 1999

Development Genes and Evolution

104

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Whole-mount technique using fluorescent-labelled phalloidin for actin staining and confocal laser scanning microscopy as well as semi-thin serial sectioning, scanning and transmission electron microscopy were applied to investigate the ontogeny of the various muscular systems during larval development in the limpets Patella vulgata L. and P. caerulea L. In contrast to earlier studies, which described a single or two larval shell muscles, the pretorsional trochophore-like larva shows no less than four different muscle systems, namely the asymmetrical main head/foot larval retractor muscle, an accessory larval retractor with distinct insertion area, a circular prototroch/velar system, and a plexus-like pedal muscle system. In both Patella species only posttorsional larvae are able to retract into the shell and to close the aperture by means of the operculum. Shortly after torsion the two adult shell muscles originate independently in lateral positions, starting with two fine muscle fibres which insert at the operculum and laterally at the shell. During late larval development the main larval retractor and the accessory larval retractor become reduced and the velar muscle system is shed. In contrast, the paired adult shell muscles and the pedal muscle plexus increase in volume, and a new mantle musculature, the tentacular muscle system, and the buccal musculature arise. Because the adult shell muscles are entirely independent from the various larval muscular systems, several current hypotheses on the ontogeny and phylogeny of the early gastropod muscle system have to be reconsidered.

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Section: Culture and Breeding Of Patellamentioning

confidence: 99%

Development of the musculature in the limpet Patella (Mollusca, Patellogastropoda)

Wanninger¹,

Ruthensteiner²,

Lobenwein

et al. 1999

Development Genes and Evolution

104

View full text Add to dashboard Cite

show abstract

“…Complete, ribboned serial sections (2 mm) were prepared using 'Ralph' glass knives and contact cement ('Pattex-compact') at the lower cutting edge (Henry, 1977). The sections were stained with methylene-blueazure ll according to Richardson et al (1960) and deposited in the Zoologische Staatssammlung Mu¨nchen (ZSM; Mollusca section; inventory numbers 20004766/1, 20004767, 20004768, 20004769); colours of tissues mentioned within the description refer to staining reactions.…”

Section: Methodsmentioning

confidence: 99%

Microanatomy Of Hedylopsis Ballantinei, A New Interstitial Acochlidian Gastropod From The Red Sea, And Its Significance For Phylogeny

Sommerfeldt

Schrödl

2005

Journal of Molluscan Studies

120

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This is the first description of an acochlidian gastropod species from the Red Sea. More than 20 specimens of Hedylopsis ballantinei n. sp. have been extracted from subtidal coral sand in the Gulf of Aqaba. As a model organism for this poorly known, highly aberrant and enigmatic opisthobranch group, the anatomy of this new species is described in detail. Major organ systems have been reconstructed from histological semithin serial sections. The central nervous system has also been studied by means of immunocytochemical staining and confocal laser scanning techniques; dorsal bodies and rhinophoral ganglia are described for the first time for Acochlidia. Hardparts were examined using SEM, the midpiece of an acochlidian sperm is described with aid of TEM for the first time. Our results show Hedylopsis ballantinei n. sp. to be similar to the European H. spiculifera (junior synonym H. suecica). Differences include tropical occurrence, larger rhinophoral ganglia, different arrangement of ampulla and genital glands, and the probable absence of anterior male genitalia. Hedylopsis ballantinei n. sp. plays an important role in understanding acochlidian phylogeny, since it combines an array of morphological features that appear plesiomorphic for Acochlidia, e.g. a (small) mantle cavity, a unidirectional stomach lacking any cuticular elements, a tube-like, holohepatic digestive gland, a prepharyngeal, epiathroid central nervous system with separate cerebral, pleural and pedal ganglia, dorsal bodies associated with the cerebral ganglia, a (short) visceral loop with three ganglia, a monaulic and hermaphroditic reproductive system, and elongate spiral sperm. On the other hand, the absence of anterior male genitalia appears to be a derived condition that is shared with microhedylacean species. The aberrant possession of three glycogen helices in the sperm midpiece appears unique for opisthobranchs. With present, limited knowledge, the Acochlidia is regarded as a monophyletic group that is supported by several autapomorphies, with a hypothetical origin from acteonoidean opisthobranchs, i.e. diaphanid-like ancestors.

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“…For light microscopy, ribbons of serial sections (1.5 m) were prepared with "Ralph" glass knives and contact cement ("Pattex-compact") at the lower cutting edge (modified from Henry, 1977). The sections were stained with methylene-blue-azure II according to Richardson et al (1960).…”

Section: Light and Electron Microscopymentioning

confidence: 99%

Fine structure and immunocytochemistry of a new chemosensory system in the chiton larva (Mollusca: Polyplacophora)

Haszprunar

Friedrich

Wanninger

et al. 2001

Journal of Morphology

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Combined electron microscopy and immunocytochemistry of the larvae of several polyplacophoran species (Chiton olivaceus, Lepidochitona aff. corrugata, Mopalia muscosa) revealed a sensory system new to science, a so-called "ampullary system." The cells of the "ampullary system" are arranged in four symmetrically situated pairs lying dorsolaterally and ventrolaterally in the pretrochal part of the trochophore-like larva and they send axons into the cerebral commissure. They are lost at metamorphosis. The fine structure of these cells strongly resembles that of so-called "ampullary cells" known from various sensory organs of other molluscs, such as the apical complex of gastropod and bivalve larvae, osphradia of vetigastropods, and olfactory organs of cephalopods, and nuchal organs of certain polychaetes. The ampullary cells and their nerves are densely stained by anti-FMRF-amide fluorescence dyes, whereas antiserotonin staining is only weak. While cytological homology of the ampullary cells with those of other organs is probable, the ampullary system as a whole is regarded as a synapomorphy of the Polyplacophora or Chitonida.

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A Method for Obtaining Ribbons of Serial Sections of Plastic Embedded Specimens

Cited by 43 publications

References 2 publications

Development of the musculature in the limpet Patella (Mollusca, Patellogastropoda)

Development of the musculature in the limpet Patella (Mollusca, Patellogastropoda)

Microanatomy Of Hedylopsis Ballantinei, A New Interstitial Acochlidian Gastropod From The Red Sea, And Its Significance For Phylogeny

Fine structure and immunocytochemistry of a new chemosensory system in the chiton larva (Mollusca: Polyplacophora)

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