A method for plate culture of anaerobic bacteria

THE ENUMERATION OF ANAEROBIC BACTERIA, AND OF CLOSTRIDIUM SPECIES IN PARTICULAR, IN FOODS

“…That this occurs with dilution counts is recognized, but it is not generally appreciated that it also applies to anaerobic colony counts. Because of the disadvantages of anaerobic jars, numerous modifications to Petri dishes have been suggested for cultivating and counting anaerobes (Streng, 1903 ;M'Leod, 1913;Huntemuller, 1924;Kadisch, 1924;Brekenfeld, 1924;Spray, 1930;Koch, 1934;Muller, 1037;van Riemsdijk, 1939;Brewer, 1942;Kaesz, 1042;Andersen, 1951;Leistner, 1955). None of the modifications has been found sufficiently convenient and reliable in routine use, except for the Morse-Kopeloff double plate (Tetrault, 1929).…”

A Review of the Development and Application of Physical and Chemical Principles in the Cultivation of Obligately Anaerobic Bacteria

“…The method of McLeod (1913) had for its object the sealing of the air space containing the culture prior to making the mixture of alkali and pyrogallol, so that all the oxygen absorbed might be derived with certainty from within the chamber. This device comprised a glass plating dish with upcurled edges, to prevent escape of infected droplets, which after streaking was to be inverted over a porcelain capsule partitioned for the receipt, on one side of pyrogallic acid, on the other strong alkali, throughi a central perforation.…”

Enhancing the conventional culture: The evaluation of several culture media and growth conditions improve the isolation of ruminal bacteria

“…The ruminal ecosystem contains a vast number of microorganisms that interact in complex ways based on the degradation of food and the provision of nutrients to the animal. Traditional culture-dependent methods, such as the roll tube technique and the use of dilutions and ruminal uid-based media, have been used to isolate ruminal microorganisms (9,10). However, these methods are limited as they only encompass 10-20% of the ruminal population.…”