This work addresses an important problem in industry -locating the bottleneck in a production line -and suggests a practical approach to accomplish that end. We describe and validate, using discrete event simulation, a novel method of bottleneck detection in open, asynchronous serial production lines with finite buffers. The technique uses a single measure -station interdeparture time variance -to locate the system bottleneck. The proposed method is compared to other bottleneck detection approaches and it is shown that the proposed method performs as well and sometimes better than other methods. We conclude that the proposed approach has a number of significant advantages. It is easy to use and implement, not requiring data about failure and repair times, raw process times, buffer sizes, etc., but instead uses a single piece of easily obtained real-time production line data -station work-in-process (WIP) interdeparture time. The proposed method can identify production constraints without the need to build an analytical or simulation model, is well suited for use in industry, and can be readily implemented in standard simulation tools.