A method for the colorimetric estimation of glycogen with lodine

Krisman, Clara R.

doi:10.1016/0003-2697(62)90014-3

Cited by 482 publications

(164 citation statements)

References 12 publications

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“…Hers et al used a similar ratio to compare different subgroups of glycogenosis [14]. The A values have an interpretation coincident with the R ratio for the same polysaccharide [6] (amount of polysaccharide determined by the Krisman's reagent/amount of polysaccharide determined by the phenol/sulfuric acid method). The values of R higher or lower than 1 indicate that the external branches are longer or shorter than the standard polysaccharide.…”

Section: Discussionmentioning

confidence: 99%

“…Properties of different (ul,4)-(cil,6)glucopolysaccharides A = AI.mar/A390. R = ratio between the amounts of polysaccharide (glucose, nmol/ml) obtained by Krisman's [6] and phenol/sulphuric acid [21] methods. The degree of branching points was estimated as described in [4].…”

Section: Discussionmentioning

confidence: 99%

“…A = AA,,,/A390. A,,, refers to the colour product obtained following treatment with Krisman's reagent [6]. The degree of branching points is based on assay of total glucose residues and those specifically linked (a1,6), determined after treatment with debranching enzyme and phosphorylase b [4] shown in Table 1.…”

Section: Liver Glycogen and Potato Starch Amylopectinmentioning

confidence: 99%

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The degree of branching in (α1,4)‐(α1,6)‐linked glucopolysaccharides is dependent on intrinsic properties of the branching enzymes

Tolmasky¹,

Krisman²

1987

European Journal of Biochemistry

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1. Branching enzymes from rat and rabbit liver, as well as from potato and maize were prepared. They were almost free from contaminating glucan-degrading enzymes.2. In 'sweet corn' maize, two separate fractions with (al,4)glucan : (al,4)glucan a6-glycosyltransferase activities were obtained. One of them synthesized amylopectin, the branched component of starch, in the presence of phosphorylase and Glcl P, while the other fraction synthesized phytoglycogen. Furthermore, in a maize variety which does not accumulate phytoglycogen, only one fraction of branching activity was found, that formed amylopectin under the above-mentioned conditions.3. Comparative analyses performed with native (al,4)-(a1,6)glucopolysaccharides, and those synthesized in vitro with the branching enzyme from the same tissue, demonstrated a close similarity between both glucans.4. It may be concluded that the branching enzyme is responsible for the specific degree of (a1,6) branch linkages found in the native polysaccharide.The branching enzyme transfers to an acceptor an (al,4)-linked glucan fragment removed from a donor by breakage of an (a1,4) bond, which may or may not be the original donor, creating an (a1,6) bond [l, 21. The reaction does not involve net glucan synthesis.The enzyme responsible for the formation of (a1,4) glucosidic bonds in vivo is the synthase, nevertheless it is also possible to create these bonds through the reverse action of phosphorylase. Much valuable information on the regulatory mechanism of glycogen synthase or phosphorylase has been published [3] but little has been reported on branching enzymes. Thus, it has been assumed that a relatively high elongation activity [synthesis of (al,4)glucosidic chains] would result in the synthesis of glycogen with a relatively low degree of branching. On the other hand, a relatively low elongation activity would allow the branching enzyme to introduce a high proportion of (al,6)glucosidic linkages [3].Polysaccharides with different numbers of (al,6)glucosidic linkages are normally found in nature; so starch amylopectins from different origins are different. Potato amylopectin is quite different from that of maize or wheat. Also, rat liver glycogen is quite different from rabbit liver glycogen or bacterial glycogen. Thus, we wondered whether the density of branching points depends on the activity of the branching enzyme, on the ratio of branching to synthase activities or if it is due to intrinsic characteristics of the branching enzyme activity.The numbers of branching points of native (a1,4)-(al,6)glucopolysaccharides were quantified and compared to those of the polysaccharides synthesized in vitro. The branching enzymes were obtained from the respective tissues and the products compared by a method developed in our laboratory [4].The results reported here show that the degree of branching is related to intrinsic properties of the branching enzymes, rather than to the ratio of elongation/branching activities.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Liver Glycogen and Potato Starch Amylopectinmentioning

confidence: 99%

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The degree of branching in (α1,4)‐(α1,6)‐linked glucopolysaccharides is dependent on intrinsic properties of the branching enzymes

Tolmasky¹,

Krisman²

1987

European Journal of Biochemistry

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“…Glycogen content, an important energy source for muscle during physical exercise, was assayed by the method described by Krisman. 30 Briefly, a known amount of tissue was digested in 30% KOH solution. Followed 10 min in boiling water bath, ethanol was added to the tubes to precipitate glycogen.…”

Section: Ex Vivo Experimentsmentioning

confidence: 99%

Antioxidant effect of diphenyl diselenide on oxidative stress caused by acute physical exercise in skeletal muscle and lungs of mice

Prigol

Luchese

Nogueira

2009

Cell Biochemistry & Function

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This study was designed to examine if diphenyl diselenide (PhSe) 2 , an organoselenium compound, attenuates oxidative stress caused by acute physical exercise in skeletal muscle and lungs of mice. Swiss mice were pre-treated with (PhSe) 2 (5 mg kg -1 day -1 ) for 7 days. At the 7th day, the animals were submitted to acute physical exercise which consisted of continuous swimming for 20 min. The animals were euthanized 1 and 24 h after the exercise test. The levels of thiobarbituric acid reactive species (TBARS), non-protein thiols (NPSH) and ascorbic acid and the activity of catalase (CAT) were measured in the lungs and skeletal muscle of mice. Glycogen content was determined in the skeletal muscle of mice. Parameters in plasma (urea and creatinine) were determined. The results demonstrated an increase in TBARS levels induced by acute physical exercise in the skeletal muscle and lungs of mice. Animals submitted to exercise showed an increase in non-enzymatic antioxidant defenses (NPSH and ascorbic acid) in the skeletal muscle. In lungs of mice, activity of CAT was increased. (PhSe) 2 protected against the increase in TBARS levels and ameliorated antioxidant defenses in the skeletal muscle and lungs of mice submitted to physical exercise. These results indicate that acute physical exercise caused a tissue-specific oxidative stress in the skeletal muscle and lungs of mice. (PhSe) 2 protected against oxidative damage induced by acute physical exercise in mice.

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“…The absorption spectrum of the polysaccharide-iodine complex was measured in a Gilford 240 recording spectrophotometer after addition of Nocardia storage Polysuccharide storage in N . asteroides 35' polysaccharide to a solution of I,-KI (Krisman, 1962). The spectrum produced was conipared with that of soluble starch (Fisher Scientific, Pittsburgh, Pennsylvania, U.S .A.…”

Section: J K D I P E R S I O a N D S J D E A L M E T H O D Smentioning

confidence: 99%

Identification of Intracellular Polysaccharide Granules in Thin Sections of Nocardia asteroides

DiPersio

Deal

1974

Journal of General Microbiology

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A method for the colorimetric estimation of glycogen with lodine

Cited by 482 publications

References 12 publications

The degree of branching in (α1,4)‐(α1,6)‐linked glucopolysaccharides is dependent on intrinsic properties of the branching enzymes

The degree of branching in (α1,4)‐(α1,6)‐linked glucopolysaccharides is dependent on intrinsic properties of the branching enzymes

Antioxidant effect of diphenyl diselenide on oxidative stress caused by acute physical exercise in skeletal muscle and lungs of mice

Identification of Intracellular Polysaccharide Granules in Thin Sections of Nocardia asteroides

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