A method for the detection and measurement of insecticide resistance in larvae of <i>Lucilia cuprina</i> (Wied.) (Dipt., Calliphoridae)

Roxburgh, N. A.; Shanahan, G. J.

doi:10.1017/s0007485300050902

Cited by 36 publications

(19 citation statements)

References 8 publications

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“…2001;Levot & Sales 2002). The method is based on that of Roxburgh & Shanahan (1973). Briefly, strips (12 by 3 cm) of chromatography paper were treated with a serial range of insecticide concentrations expected to elicit 0-100% mortality of larvae.…”

Section: Diflubenzuronmentioning

confidence: 99%

Insect growth regulator cross‐resistance studies in field‐ and laboratory‐selected strains of the Australian sheep blowfly, Lucilia cuprina (Wiedemann) (Diptera: Calliphoridae)

Levot

Sales

2004

Australian Journal of Entomology

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Resistance to diflubenzuron in the Australian sheep blowfly, Lucilia cuprina , has rendered this insecticide incapable of preventing flystrike in sheep from a few districts in eastern Australia. Wool producers affected by this situation must find suitable alternatives to protect their flocks. Results of laboratory bioassays against one population demonstrated that, despite extremely high diflubenzuron resistance (Resistance Factor > 791), it had only very low (2x) tolerance of cyromazine and dicyclanil. It is unlikely that this level of tolerance would have any practical impact on field control with either insecticide. Consequently, wool producers in districts where diflubenzuron-resistant flies are common can rotate insecticide treatment to either of these compounds to prevent flystrike in their flocks. However, unlike the highly diflubenzuron-resistant field strain, a laboratory strain selected for resistance to diflubenzuron (Resistance Factor = 617) was 10 times more resistant to dicyclanil than a susceptible strain but, like the field strain, was only two times more tolerant of cyromazine. Conversely, a field-derived strain selected in the laboratory for cyromazine resistance was 20 times more resistant to dicyclanil and 362 times more resistant to diflubenzuron than the reference susceptible strain.

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Section: Diflubenzuronmentioning

confidence: 99%

Insect growth regulator cross‐resistance studies in field‐ and laboratory‐selected strains of the Australian sheep blowfly, Lucilia cuprina (Wiedemann) (Diptera: Calliphoridae)

Levot

Sales

2004

Australian Journal of Entomology

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“…observed in other tests in Lucilia (Roxburgh and Shanahan, 1973;Arnold and Whitten, 1975) and in insects generally (Brown and Pal, 1971) in that resistance displayed a partially dominant mode of inheritance ( fig. 1).…”

Section: ±094mentioning

confidence: 75%

Frequency-dependent selection at the diazinon resistance locus of the Australian sheep blowfly, Lucilia cuprina

1986

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Frequency-dependent selection at the diazinon resistance locus of the Australian sheep blowfly, Lucilia cuprinaThe egg to adult viability of susceptibles (SS) at the diazinon resistance locus of L. cuprina was facilitated by RS in mixed (1: 1) cultures at diazinon concentrations greater than O•0002 per cent (W/V) in the concentration range O-OOOO53 per cent (W/V). SS survived in mixed culture at concentrations lethal to pure cultures of this genotype. The egg to adult viability of RS was not influenced by the presence of SS.Trials at 0 per cent and 00002 per cent (W/V) diazinon showed the viability of both genotypes decreased in the presence of diazinon and with increasing density. Viability of RS was independent of its frequency in mixed culture but SS viability was enhanced at frequencies 20 per cent at the 00002 per cent (W/V) concentration. There was no indication of density x frequency interaction.

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“…Bioassays were conducted using a modiÞcation of the method of Roxburgh and Shanahan (1973). Groups of 50 newly-hatched larvae were washed into tubes containing strips of chromatography paper using 1 ml of sheep serum fortiÞed with 2% yeast extract and stabilized with 0.5% potassium orthophosphate.…”

Section: Methodsmentioning

confidence: 99%

Inheritance of Diflubenzuron Resistance and Monooxygenase Activities in a Laboratory-Selected Strain of <I>Lucilia cuprina</I> (Diptera: Calliphoridae)

Kotze

Sales

2001

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Inheritance of the high-level diflubenzuron resistance shown by a laboratory-selected strain of Lucilia cuprina (Wiedemann) was examined in matings with a susceptible reference strain. Progeny of reciprocal crosses between resistant females and susceptible males showed higher LC50 values than the alternate reciprocal cross, indicating some maternal influence on inheritance of resistance. Resistance was inherited in a codominant (S male x R female) or incompletely recessive (R male x S female) manner. Monooxygenase activities (aldrin epoxidation) of the F1 generations were also intermediate between the levels shown by the parental lines, however, inheritance of enzyme activities showed greater degrees of dominance than for resistance levels. There was also some maternal influence on inheritance of monooxygenase activities. Backcrosses of F1 generations to both susceptible and resistant parents did not fit the expected patterns for a major sex-linked resistance locus, indicating that the maternal influence on resistance inheritance was not associated with sex-linkage of a major resistance gene. The backcross data also failed to fit the model for a single major autosomal gene, suggesting that the resistance in the diflubenzuron-selected strain is polygenic, involving mechanisms additional to monooxygenases.

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A method for the detection and measurement of insecticide resistance in larvae of Lucilia cuprina (Wied.) (Dipt., Calliphoridae)

Cited by 36 publications

References 8 publications

Insect growth regulator cross‐resistance studies in field‐ and laboratory‐selected strains of the Australian sheep blowfly, Lucilia cuprina (Wiedemann) (Diptera: Calliphoridae)

Insect growth regulator cross‐resistance studies in field‐ and laboratory‐selected strains of the Australian sheep blowfly, Lucilia cuprina (Wiedemann) (Diptera: Calliphoridae)

Frequency-dependent selection at the diazinon resistance locus of the Australian sheep blowfly, Lucilia cuprina

Inheritance of Diflubenzuron Resistance and Monooxygenase Activities in a Laboratory-Selected Strain of <I>Lucilia cuprina</I> (Diptera: Calliphoridae)

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