A method for the determination of insulin by paper chromatography

Bouman, Jg; Homan, Jens

doi:10.1016/0006-3002(58)90201-4

Cited by 10 publications

(4 citation statements)

References 19 publications

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“…20/150 filter paper 8 with the organic phase of a 3:1:4 mixture of n-butanol:acetic acid:water. 9 This system was chosen on the basis of its ability to resolve the components of a mixture consisting of insulin, 3-iodotyrosine, 3,5-diiodotyrosine, and inorganic iodide. No evidence has been found for the lability of organically bound iodine in this system.…”

Section: Methodsmentioning

confidence: 99%

Metabolism of Insulin-I131 Studies in Isolated, Perfused Rat Liver and Hind-limb Preparations

Mortimore¹,

Tietze²,

Stetten³

1959

Diabetes

105

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Section: Methodsmentioning

confidence: 99%

Metabolism of Insulin-I131 Studies in Isolated, Perfused Rat Liver and Hind-limb Preparations

Mortimore¹,

Tietze²,

Stetten³

1959

Diabetes

105

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“…The Latin-square design balanced out positional and paper effects, since each spot of test and standard occurred once on each paper and occupied all available positions over the four papers. Several unsuccessful attempts were made to separate insulin from crystalline glucagon (Staub, Sinn & Behrens, 1953, by the chromatographic method as described and by the radial technique of Bouman & Homan (1958). Although the latter procedure gave somewhat better separation in our hands than the ascending method, there was still some overlapping of the spots.…”

Section: Resultsmentioning

confidence: 80%

“…The co-ordinate values, after transformation to logarithms to base 10, gave X = 1.08 Y 0 12 and Y = 0 885X 0 173 for the best-fitting lines. (Fenton, 1959) and temperature fluctuations (Bouman & Homan, 1958). These effects had to be compensated by the presence of standards on every paper in each assay.…”

Section: Resultsmentioning

confidence: 99%

“…The quantitative determination of insulin by paper chromatography has been the subject of numerous publications in recent years. Using the system butanol-acetic acid-water, in varying proportions, Light & Simpson (1956a, b) and Grodsky & Tarver (1956) separated insulin from crude pancreatic and liver proteins, Robinson & Fehr (1952) developed a method for the estimation of insulin and protamine in protamine zinc-insulin preparations, and Bouman & Homan (1958) determined insulin potency by radial chromatography. Fenton (1959) described a method for the chromatographic assay of crystalline insulin by using the solvent system butan-2-ol-1 % acetic acid (1:1, v/v).…”

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confidence: 99%

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The assay of insulin solutions by paper chromatography

Fenton¹

1961

Biochemical Journal

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1961aldehyde. Vitamin A1 aldehyde was the predominant form in all except the freshwater perch, pike, and trout and the anadromous salmon, which contained predominantly vitamin A2 aldehyde.3. Values are given for the conteiut of total vitamin A1 and A2 esters and alcohols, the percentage of the A2 form and the carotenoid content of the eggs or ovaries.We are grateful to the many people who have helped with the collection of samples, and thank Dr L. R. Fisher for his help in zoological matters and Mr C. M. Ward for technical assistance.

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