A method for the determination of cholesterol in blood serum

Klungsöyr, Leiv; Haukenes, Else; Closs, Karl

doi:10.1016/0009-8981(58)90002-0

Cited by 27 publications

(7 citation statements)

References 2 publications

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“…After marking the areas the iodine was allowed to sublime and the bands scraped into 10 ml graduated tubes for analysis. Cholesterol and cholesteryl esters were estimated directly in the presence of silicic acid by the method of Zlatkis, Zak and Boyle [1953] modified by Klungsoyr, Haukenes and Closs [1958]. Triglycerides were extracted by shaking with 10 ml methanol/chloroform (1:1 v/v) and a suitable volume was taken for analysis by the method of Carlson and Wadstrom [1959].…”

Section: Methodsmentioning

confidence: 99%

Plasma Lipoproteins of Lambs and Sheep

1976

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The major plasma lipoproteins of the adult sheep were high density lipoprotein (76%) having α‐mobility on electrophoresis and low density lipoprotein (20%) having β‐mobility. Chylomicrons and very low density lipoproteins were minor constituents (<5%). The postabsorptive hyperlipidaemia in suckling lambs is mainly a result of increased concentration of low density and high density lipoproteins although the relative contribution of very low density lipoproteins was increased to 7—15% of the total lipoproteins. The hyperlipidaemia was markedly greater in an intact male lamb than in female or castrated male lambs. In suckling lambs a new lipoprotein (density 1·090 g/ml) appeared in the high density lipoprotein fraction but disappeared before weaning.

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Section: Methodsmentioning

confidence: 99%

Plasma Lipoproteins of Lambs and Sheep

1976

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“…Our results seem to agree fairly well with those from similar studies (Tables I1 and 111). Assaying cholesterol by the method of Klung-s@yr et al (26) is known to give results on the average 14% higher than those obtained by Schoenheimer-Sperry's method. Correcting for this methodological difference, the mean serum cholesterol value in our study is 149+25 m g % , clearly less than reported by most workers cited in Table IT, but in close approximation to that of Carlson et al (12).…”

Section: Discussionmentioning

confidence: 86%

“…wand. 190 Lipid analyses Total cholesterol was estimated by the ferric chloride method of Klungseyr et al (26) and triglycerides were analysed according to Dunsbach (17). The precision of the methods was calculated from 58 replicate analyses of the same serum according to the formula: where d = difference between duplicates, and N = total number of determinations.…”

Section: No Trainingmentioning

confidence: 99%

Distribution of Serum Lipids in Norwegian Recruits

Romslo

1971

Journal of Internal Medicine

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Abstract. 324 young men attending medical examination for military service had their sera tested for cholesterol and triglycerides. The subjects were grouped according to physical fitness, relative weight, smoking habits, blood pressure and ischaemic heart disease among relatives. Serum triglyceride values were found correlated to relative weight, smoking habits and ischaemic heart disease among relatives. A similar, but less obvious, trend was observed in serum cholesterol.

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“…Rabbit antisera of different specificity (anti-y, anti-pz~ and anti-pm) were used in the study of the immune-globulins. The serum cholesterol was measured by the method of Klungsoyr et al (1958).…”

Section: Methodsmentioning

confidence: 99%