A method for the determination of perfluorooctanoic acid in blood and other biological samples

Belisle, Jon; Hagen, D.F.

doi:10.1016/0003-2697(80)90202-x

Cited by 61 publications

(43 citation statements)

References 7 publications

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“…The phosphate buffer gave the best results in terms of efficiency (N) and resolution (R s ) for the two examined PFCAs. Small numbers of theoretical plates (N) for PFCAs can most likely be attributed to the tendency for strong adsorption of these compounds to surfaces as indicated in the literature [16,29].…”

Section: Optimization Of the Bgementioning

confidence: 97%

“…Most of the methods described in the literature involve chromatographic separation, but also methods without chromatographic separation have been developed, such as 19 F-NMR, requiring, however, preconcentration of analytes [10], attenuated total reflection -FTIR [11], and mass spectrometry (MS) with direct injection, which was employed for determination of various PFAS anions in natural waters [12]. Determinations of PFASs by gas chromatography (GC) with electron capture detection [13], mass spectrometry [14], and flame ionization detection [15] has been utilized. The GC-based methods are limited by the necessity to form ester derivatives of perfluorocarboxylic acids (PFCAs) before analysis and hence complex sample preparation procedures that are prone to losses of derivatized analytes.…”

Section: Introductionmentioning

confidence: 99%

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Separation of perfluorocarboxylic acids using capillary electrophoresis with UV detection

et al. 2005

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A capillary electrophoretic method with UV detection for separation and quantitation of perfluorocarboxylic acids (PFCAs) from C6-PFCA to C12-PFCA has been developed. The optimization of measurement conditions included the choice of the most appropriate type and concentration of buffer in the background electrolyte (BGE), as well as the type and the content of an organic modifier. The optimal separation of investigated PFCAs was achieved with 50 mM phosphate buffer and 40% isopropanol in the BGE using direct UV detection. The optimum wavelength for direct UV detection was optimized at 190 nm. For indirect detection, several chromophores were studied. Five mM 3,5-Dinitrobenzoic acid (3,5-DNBA) in 20 mM phosphate buffer BGE and indirect UV detection at 280 nm gave the optimal detection and separation performance for the investigated PFCAs. The possibility of on-line preconcentration of solutes by stacking has been examined for indirect detection. The detection limits (LODs) determined for direct UV detection ranged from 2 microg/mL for C6-PFCA to 33 microg/mL for C12-PFCA. The LODs obtained for indirect UV detection were comparable to those obtained for direct UV detection.

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Section: Optimization Of the Bgementioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Separation of perfluorocarboxylic acids using capillary electrophoresis with UV detection

et al. 2005

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“…Organofluorine in human blood was first reported in 1968 (20) and has since been measured in human blood in the United States and other countries (21). Earlier, it was postulated that the source was PFOA or a structurally related compound.…”

Section: Occurrence In Humansmentioning

confidence: 99%

Peer Reviewed: Perfluorochemical Surfactants in the Environment

Giesy¹,

Kannan²

2002

Environ. Sci. Technol.

961

491

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“…Numerous analytical methods have been introduced to measure PFC concentrations with both precision and accuracy in biological and environmental samples. Several analytical tools for measurement of PFCs include liquid chromatography (LC) using spectrophotometeric detection, 11,12 19 F-NMR, 13 gas chromatography-mass spectrometry (GC-MS), 14 and LC-MS 15 or tandem mass spectrometry (MS/MS). [6][7][8][9][10] Among them, a LC-MS/MS based on MRM method has been recommended to minimize interference effects in complex sample matrices, enabling to enhance sensitivity, selectivity, and specificity.…”

Section: Introductionmentioning

confidence: 99%

High Speed Separation of PFCs in Human Serum by C18-Monolithic Column Liquid Chromatography-Tandem Mass Spectrometry

Lee¹,

Lee²,

Yu³

et al. 2012

Bulletin of the Korean Chemical Society

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An analytical method has been developed for the rapid determination of perfluorinated compounds (PFCs) in human serum samples. The extraction and purification of PFCs from human serum were performed by the modified method of previous report. Ten PFCs were rapidly separated within 3.3 min by C18-monolithic column liquid chromatography (LC) and detected by electrospray ionization (ESI) tandem mass spectrometry (MS/MS) in negative ion mode. The runtime of PFCs on monolithic column LC was up to 4-fold faster than that on conventional column LC. The effect of triethylamine (TEA) to the mobile phase has investigated on the overall MS detection sensitivity of PFCs in ESI ionization. Quantification was performed by LC-MS/MS in multiple-ion reaction monitoring (MRM) mode, using 13 C-labeled internal standards. Method validation was performed to determine recovery, linearity, precision, and limits of quantification, followed by, the analysis of a standard reference material (SRM 1957 from NIST). The overall recoveries ranged between 81.5 and 106.3% with RSDs of 3.4 to 16.2% for the entire procedure. The calibration range extended from 0.33 to 50 ng mL . This approach can be used for rapid and sensitive quantitative analysis of 10 PFCs in human serum with high performance and accuracy.

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A method for the determination of perfluorooctanoic acid in blood and other biological samples

Cited by 61 publications

References 7 publications

Separation of perfluorocarboxylic acids using capillary electrophoresis with UV detection

Separation of perfluorocarboxylic acids using capillary electrophoresis with UV detection

Peer Reviewed: Perfluorochemical Surfactants in the Environment

High Speed Separation of PFCs in Human Serum by C18-Monolithic Column Liquid Chromatography-Tandem Mass Spectrometry

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