A method for the estimation of bacterial biomass and community structure in mangrove-associated sediments

Gillan, F.T.; Hogg, Ronald W.

doi:10.1016/0167-7012(84)90023-x

Cited by 99 publications

(41 citation statements)

References 22 publications

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“…Several studies have revealed that branched, monounsaturated, cyclopropyl fatty acids and also certain saturated fatty acids present in sediments are definitely contributed by in situ bacterial production (Perry et al 1979, Gillan & Hogg 1984, White et al 1984. Furthermore, we detected other fatty acids, which are known to be present either in both prokaryotes and microeukaryotes or only in microeukaryotes.…”

Section: Discussionmentioning

confidence: 99%

Phospholipid fatty acid profiles at depositional and non-depositional sites in the North Sea

Stoeck¹,

Kröncke²,

Duineveld³

et al. 2002

Mar. Ecol. Prog. Ser.

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The distribution of ester-linked phospholipid fatty acid (PLFA) in sediments of 12 sampling stations located in the German Bight, Skagerrak/northern Kattegat, Frisian Front, Oyster Ground, eastern North Sea and the Dogger Bank area was studied to compare the in situ microbial community structure of depositional and non-depositional sites. A total of 36 fatty acids in the range of C 12 to C 24 were determined. They consisted of saturated, branched, monounsaturated, polyunsaturated and hydroxy fatty acids, and variation was revealed in the relative proportions (mol %) of these fatty acids. The distribution of specific fatty acids was significantly different between depositional and non-depositional sites. Additionally, we could discriminate communities of sites which are intermediate in sedimentation regime. Highest total PLFA content was found at depositional sites. The PLFA profiles of all sites were dominated by bacteria, with highest bacterial contribution to the sedimentary PLFA pool at depositional sites. A high proportion of aerobic prokaryotes (up to 40%) at depositional sites indicated the occurrence of aerobic microniches in the corresponding anoxic sediments. Bacterial groups being a component of the degradation pathways of complex macromolecules (e.g. Cytophaga and Actinomycetes) were found in significant higher abundances at depositional sites compared to the other sites. At depositional sites, sulphate-reducing bacteria (SRB) (e.g. Desulfobacter spp.) were more abundant compared to the other stations. The relative contribution of microeukaryotes to the sedimentary PLFA pool was significantly higher at depositional sites. A nutritionally limited benthic system at non-depositional sites in the offshore is indicated by a microbial community exposed to higher physiological stress than those of the organically enriched sediments of the depositional sites.

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Section: Discussionmentioning

confidence: 99%

Phospholipid fatty acid profiles at depositional and non-depositional sites in the North Sea

Stoeck¹,

Kröncke²,

Duineveld³

et al. 2002

Mar. Ecol. Prog. Ser.

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“…Briefly, bacterial numbers (1 cm depth) were estimated from replicate core samples at each site (0.2 g dry wt sediment c o r e ' , n = 3) using epifluorescence microscopy (Hobbie et al 1977). A value of 1.7 X 10"14 gC cell"' was used to convert bacterial numbers to carbon (Rublee 1982, Gillan & Hogg 1984. Bacterial production was measured by the rate of [H-methyl]thymidine incorporation into DNA (Pollard & Moi-iarty 1984, Alongi 1988a) from 9 replicate sediment cores (each 0.38 cm3).…”

Section: Methodsmentioning

confidence: 99%

“…Recent studies (Gillan & Hogg 1984, Alongi 1988a) have demonstrated very high densities and productivity of bacteria in mangrove sediments of tropical Australia, with abundances comn~only exceeding 10'' cells per g dry wt of sediment, and bacterial production averaging ca 1.6gC m d ' or 2 to 10 times higher than has been reported for other marine sediments (Fallon et al 1983, Moriarty 1986). Sedimentary bacterial populations in tropical mangroves appear to be controlled mainly by physical factors such as temperature and degree of tidal inundation, with apparently very little consumption by the low meiofaunal populations in these sediments (Alongi 1988a,b).…”

Section: Introductionmentioning

confidence: 96%

“…It was hypothesized, therefore, that the much higher densities of bacteria in the surface sediments of tropical mangroves (Gillan & Hogg 1984, Alongi 1988a) act as a very effective sink for the porewater DOC, giving rise to negligible export from the system. This study reports on the DOC concentrations found in the porewaters of tropical mangrove sediments and their variations with depth, tidal elevation and season.…”

Section: Introductionmentioning

confidence: 99%

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Dissolved organic carbon-bacteria interactions at sediment-water interface in a tropical mangrove system

Boto¹,

Alongi²,

Nott³

1989

Mar. Ecol. Prog. Ser.

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In order to determine the efficiency of in situ carbon recycling in tropical mangrove sediments, and the contribution of porewater dissolved organic carbon (DOC) to surface-sediment bacterial production, concentrations and fluxes of DOC were measured and compared with simultaneous estimates of bacterial densities, productivity and specific growth rates, in a mangrove forest in Queensland, Australia. The range of interstitial DOC concentrations (4 to 50mgC 1 ' ) was similar to that reported for temperate marine sediments although seasonal variations were much less apparent. Soluble tannins constituted 2 to 51 % of the interstitial DOC pools, which varied significantly with tidal elevation (high > mid > low intertidal) and showed significant variation with sediment depth (to 1 m) only in the mid intertidal. Bacterial densities and activity in surface sediments (to 1 cm) were high (annual range in productivity: 0.9 to 4.1 gC m-* d ' ) , consistent with previous estimates at this site. Surface (1 cm depth) sediment DOC concentrations varied with elevation in the intertidal zone (10 sites from low to high intertidal), and showed no correspondence with seasonal or spatial variations in bacterial parameters, except for a significant correlation (r= 0.71, n = 10) between DOC and bacterial growth rate, in austral autumn, for samples throughout the tidal range. Despite the high concentration gradient of DOC between porewaters and overlying tidal waters, significant efflux of DOC was rarely detected unless sediments were poisoned, to 1 cm depth, with mercury, which resulted in significant and generally high rates of efflux. Efflux rates varied widely with tidal elevation and season (range: 0 to 2 . 4 gC m-' d"') These DOC flux rates provide, on average, 35 % (range: 0 to 100 O h ) of bacterial productivity requirements at the sediment-water interface. This study suggests that the mangrove sediments function as an efficient sink for DOC and that its export from the system is minor.

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“…The trans to cis ratios appear to indicate the stress experienced by the microorganisms. The ratios in most bacterial and sediment samples were found to be less than 0.1 (Gillan and Hogg, 1984;Guckert et al, 1985), but the ratio was greater than 1 during starvation (Mancuso et al, 1990). It has been suggested that the trans/cis ratio of MUFA may be useful as an index of stress for the determination of the nutritional status of bacteria in aquatic environments.…”

Section: Community Structure Of Eight Different Mediamentioning

confidence: 99%

Comparative study of microbial community structure in different filter media of constructed wetland

Zhou

Tao

et al. 2010

Journal of Environmental Sciences

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A method for the estimation of bacterial biomass and community structure in mangrove-associated sediments

Cited by 99 publications

References 22 publications

Phospholipid fatty acid profiles at depositional and non-depositional sites in the North Sea

Phospholipid fatty acid profiles at depositional and non-depositional sites in the North Sea

Dissolved organic carbon-bacteria interactions at sediment-water interface in a tropical mangrove system

Comparative study of microbial community structure in different filter media of constructed wetland

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