A method for the incorporation of ovalbumin into immune stimulating complexes prepared by the hydration method

Könnings, Stephanie; Copland, Melissa J; Davies, Nigel; Rades, Thomas

doi:10.1016/s0378-5173(02)00270-3

Cited by 40 publications

(15 citation statements)

References 5 publications

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“…RYLO MG 20 constitutes a similar composition to other GMO‐based products published in the literature with its phase behaviour shown to be representative of GMO 20, 21. Ovalbumin (Ova) (chicken egg derived, grade V, Sigma, St. Louis, Missouri) was conjugated to fluorescein isothiocyanate (FITC) (Isomer I, Sigma) as described previously 22. Triton® X‐100 (octylphenol‐polyethylene glycol ether) and phosphate buffered saline (PBS) sachets (pH 7.4, 0.01 M) were obtained from Sigma–Aldrich (Auckland, New Zealand).…”

Section: Methodsmentioning

confidence: 99%

Liquid Crystalline Systems of Phytantriol and Glyceryl Monooleate Containing a Hydrophilic Protein: Characterisation, Swelling and Release Kinetics

Rizwan

Hanley

Boyd

et al. 2009

Journal of Pharmaceutical Sciences

116

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Section: Methodsmentioning

confidence: 99%

Liquid Crystalline Systems of Phytantriol and Glyceryl Monooleate Containing a Hydrophilic Protein: Characterisation, Swelling and Release Kinetics

Rizwan

Hanley

Boyd

et al. 2009

Journal of Pharmaceutical Sciences

116

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“…For release and encapsulation efficiency studies, fluorescein-labelled OVA (FITC-OVA) were utilized. FITC-OVA was prepared according to the protocol described by Könnings et al (25). The chitosan particles loaded with FITC-OVA, as well as with or without Quil-A, were prepared in the same way as described above.…”

Section: Encapsulation Efficiency and Release Of Ova In The Chitosan mentioning

confidence: 99%

Preparation and Characterization of an Oral Vaccine Formulation Using Electrosprayed Chitosan Microparticles

et al. 2018

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Chitosan particles loaded with the antigen ovalbumin (OVA) and the adjuvant, Quil-A were produced by electrospray, using mixtures of water/ethanol/acetic acid as a solvent. Three different chitosan's designed as HMC + 70, HMC + 85 and HMC + 90 (designated as 705010, 855010 and 905010) were tested and its efficacy to be used in oral vaccine delivery applications was investigated. The morphology, size and zeta potential of the produced particles were investigated, together with the encapsulation efficiency and release of OVA from the three chitosan formulations. Moreover, the mucoadhesion and cytotoxicity the chitosan microparticles was examined. All the three formulations with OVA and Quil-A were in the micrometer size range and had a positive zeta potential between 46 to 75 mV. Furthermore, all the three formulations displayed encapsulation efficiencies above 80 % and the release of OVA over a period of 80 h was observed to be between 38-47 %. None of the developed formulations exhibited high mucoadhesive properties, either cytotoxicity. The formulation prepared with HMC + 70, OVA and Quil-A had the highest stability within 2 h in buffer solution, as measured by dynamic light scattering. The electrosprayed formulation consisting of HMC + 70 with OVA and Quil-A showed to be the most promising as an oral vaccine system.

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“…Chitosan particles were loaded with fluorescein isothiocyanatecoupled OVA (FITC-OVA) prepared as described previously (Könnings et al 2002) or OVA (Grade V, purity approximately 98%; Sigma, Australia) by incubating 100 mg chitosan in a 20% (w/w of chitosan) aqueous FITC-OVA or OVA solution at 4 C for 12 h. Unbound FITC-OVA or OVA was removed by centrifuging the particles twice as described above. The resulting FITC-OVA-CNP or OVA-CNP dispersions were frozen and freeze-dried for 48 h (Freezone 6 freeze-dryer; Labconco, MO, USA).…”

Section: Loading Of Cnpmentioning

confidence: 99%

Comparison of chitosan nanoparticles and chitosan hydrogels for vaccine delivery

et al. 2008

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In this work the potential of chitosan nanoparticles (CNP) and thermosensitive chitosan hydrogels as particulate and sustained release vaccine delivery systems was investigated. CNP and chitosan hydrogels were prepared, loaded with the model protein antigen ovalbumin (OVA) and characterised. The immunostimulatory capacity of these vaccine delivery systems was assessed in-vitro and in-vivo. Particle sizing measurements and SEM images showed that optimised OVA-loaded CNP had a size of approximately 200 nm, a polydispersity index < 0.2, and a positive zeta-potential of approximately 18 mV. The amount of OVA adsorbed onto CNP was high with an adsorption efficacy of greater than 96%. Raman spectroscopy indicated conformational changes of OVA when adsorbed onto the surface of CNP. Uptake of the dispersions and immunological activation of murine dendritic cells in-vitro could be demonstrated. Investigation of the release of fluorescently-labelled OVA (FITC-OVA) from CNP and chitosan hydrogels in-vitro showed that approximately 50% of the total protein was released from CNP within a period of ten days; release of antigen from chitosan gel occurred in a more sustained manner, with < 10% of total protein being released after 10 days. The slow release from gel formulations may be explained by the strong interactions of the protein with chitosan. While OVA-loaded CNP showed no significant immunogenicity, formulations of OVA in chitosan gel were able to stimulate both cell-mediated and humoral immunity in-vivo.

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A method for the incorporation of ovalbumin into immune stimulating complexes prepared by the hydration method

Cited by 40 publications

References 5 publications

Liquid Crystalline Systems of Phytantriol and Glyceryl Monooleate Containing a Hydrophilic Protein: Characterisation, Swelling and Release Kinetics

Liquid Crystalline Systems of Phytantriol and Glyceryl Monooleate Containing a Hydrophilic Protein: Characterisation, Swelling and Release Kinetics

Preparation and Characterization of an Oral Vaccine Formulation Using Electrosprayed Chitosan Microparticles

Comparison of chitosan nanoparticles and chitosan hydrogels for vaccine delivery

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