A method for the isolation of nuclei from the dermatophytic fungus,Microsporum gypseum

Dill, Barbara; Stock, J. J.

doi:10.1007/bf00590184

Cited by 5 publications

(2 citation statements)

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“…It was dissolved in 10-fold diluted SSC (15 mM-NaC1, 1.5 mM-trisodium citrate, pH 7.0) and brought to 0.15 M with respect to NaCl and 0.015 M with respect to trisodium citrate. Pancreatic RNAase and RNAase T1 were added to final concentrations of 50 pg ml-and 10 units ml-l , respectively and incubated for 30 min at 37 "C. NaCl was added to concentrations of 1 M and DNA was re-precipitated with ethanol (95%, v/v) and estimated by the method of Giles & Myers (1965). For comparison, mycelium grown for 72 h was ground in liquid nitrogen using lysis buffer and centrifuged at 16 300g for 10 min.…”

Section: T Srikantha a N D G R A M A N A N D A Raomentioning

confidence: 99%

“…An attempt to demonstrate the presence of histones in Microsporum gypseum was unsuccessful due to poor yields of DNA (Leighton et a)., 1971). Our initial efforts to obtain DNA from Microsporum canis in sufficient quantities by the method of Dill & Stock (1974) also yielded low recoveries. Subsequently, we developed a simple and efficient method (unpublished) for the isolation of nuclear DNA from mycelium of M .…”

Section: Introductionmentioning

confidence: 99%

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A Method for Isolation of Protoplasts from Dermatophytes

Srikantha¹,

Rao

1984

Microbiology

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Section: T Srikantha a N D G R A M A N A N D A Raomentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Method for Isolation of Protoplasts from Dermatophytes

Srikantha¹,

Rao

1984

Microbiology

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Unique amanitin resistance of RNA synthesis in isolated nuclei from Amanita species accumulating amanitins

Johnson

Preston

1979

Arch. Microbiol.

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In order to understand the basis upon which amanitin-accumulating species of Amanita are able to develop in the presence of these specific inhibitors of RNA synthesis, the in vitro RNA synthesizing activities of nuclei isolated from amanitin-accumulating species. Amanita hygroscopica (culture, derived from amanitin-accumulating carpophore) and A. suballiacea (carpophore), and from the non-accumulating species A. solitaria (culture) and A. brunnescens (carpophore) were tested for their sensitivities to alpha-amanitin inhibition. The nuclear RNA synthesizing activities obtained from both carpophores and cultures of non-accumulating organisms displayed significant sensitivities to alpha-amanitin, whereas those obtained from accumulating organisms displayed remarkable resistance to alpha-amanitin. The observed relationship between levels of amanitins in carpophores and resistance of the RNA synthesizing activities to inhibition by alpha-amanitin supports the hypothesis that amanitins may function as regulators of mRNA transcription in Amanita species.

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