A method for turbidimetric measurement of bacterial growth in liquid cultures and agar plug diffusion cultures, using standard test tubes

Antimicrobial Activity and Characterization of Bacteriocin of Acetobacter tropicalis S3O1: A Novel Methionine Producing Bacteria Isolated from Sago Industrial Waste

“…The culture was incubated at 28ºC in modified MRS broth and samples were taken every 6 h to determine bacteriocin production (AU/ml) and simultaneously the growth pattern of the bacterial isolate was measured following the turbidimetric method of Brown (1980). In order to quantify the yield of bacteriocin, cell-free supernatants were serially diluted in modified MRS broth before loading 5 μl of each dilution onto the indicator strain Bacillus cereus (MTCC 1272).…”

β-2-Thienylalanine effects on growth of Escherichia, Shigella, and Salmonella cultures in liquid media containing phenylalanine, tyrosine, magnesium or zinc

“…A complex medium, Trypticase Soy Broth (BBL, CockeysviUe, MD), containing about 0.76 g Phe/1 and 0.45 g Tyr/1 [18], was used for comparison studies. 5 ml of sterile medium with test reagents added, were inoculated in duplicate or triplicate with logarithmic phase cultures, and turbidity was measured directly in the sterile test tubes [19]. Measurements were made at 3 or 4 hourly intervals, for 48 to 60 h. Different species of the same genus reacted similarly in Plate assays [6], and grew similarly in liquid media (unpublished results).…”

Comparison of Liquid and Agar‐Solidified Defined Media Regarding the Physiological Mechanism by which β‐2‐Thienylalanine Inhibits Growth of Escherichia, Shigella, and Salmonella Cultures

“…To assay liquid cultures and agar plug diffusion cultures, a technique was employed for reading culture growth in standard glass test tubes directly in the spectrophotometer (1). The turbidimetric readings were previously shown to be directly proportional to the number of colonies formed (2).…”