The stiffness of mounting system determines the vibration isolation ability of the transmitted path, which is the key factor that affects the vibration and noise of vehicle. In order to improve the vibration isolation ability of the powertrain mounting system, considering the powertrain of front wheel drive car as the research object, the vibration decoupling rate and its corresponding frequency of the powertrain mounting system are analyzed by rigid body dynamics and energy method. The correctness of the calculation program with energy method has been verified by calculated vibration decoupling rate. Based on the genetic algorithm and the fusion robustness analysis, the decoupling rate and modal frequency of the mountings in all directions are considered as the objectives; the stiffness of the three mountings is optimally designed. Through multi-excitation of three methods and vehicle test, the vibration response characteristics and the vibration noise test data of the optimization stiffness are compared and the results shown that the vibration isolation performance has been significantly improved more than 10%. An integrated design method of stiffness optimization design and vibration analysis in vehicle PMS is formed, which has theoretical and practical value, and can reduce vehicle vibration and noise.