A method of laser dark-field illumination for equal-probability excitation and photobleaching of randomly oriented fluorescent molecules

Klimov, A. A.; Klimov, D. A.

doi:10.1134/s0006350909050194

Cited by 2 publications

(1 citation statement)

References 13 publications

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“…Improvement of resolution for thick samples faces a number of problems related to physical properties of interaction of light with fluorescent molecules. Our current development efforts are aimed at solving these problems which will allow to assemble high-resolution, low-noise device for 3D color nanoscale resolution imaging of thick samples in liquid (native) environment [5]. Such developments may lead to major reduction of device cost, since expensive EMCCD-cameras with their unchallenged characteristics become less important for the improved schemes.…”

Section: Fluorescent Nanoscope Developed By Stereonic Llcmentioning

confidence: 99%

Fluorescent Nanoscope – experience of transferring science to business

Klimov¹

2010

Ions 8

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Late 90's have met with increasing interest to nano-bio systems in basic and applied research, but researchers were limited in their ability to visualize structures and processes on nanometer scale. Stereonic LLC develops a nanometer-scale-resolution fluorescent microscopy technology that can be used in a number of different areas of research, diagnostics, quality assurance etc. The technology is based on patented photoactivation-excitation-registrationdepletion principle that can be applied for a wide range of fluorescent dyes including caged fluorescein-and rhodamine-based dyes and photoactivatable proteins. The talk will give an overview of the state of art in the industry, our current developments and share an experience of transferring science to business.

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Section: Fluorescent Nanoscope Developed By Stereonic Llcmentioning

confidence: 99%