A method of preparing mesoporous Zr-based MOF and application in enhancing immobilization of cellulase on carrier surface

Wu, Jiacong; Wang, Yi; Han, Juan; Wang, Lei; Li, Chunmei; Mao, Yanli

doi:10.1016/j.bej.2022.108342

Cited by 17 publications

(2 citation statements)

References 55 publications

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“…The specific pore structure of these MOFs (the presence of mesopores along with micropores) did not affect the mass transfer and allowed for an enrichment effect of the substrate, probably due to positioning of the latter at the support. An addition of 4.6 nm mesopores in microporous Zr-MOF obtained by biomineralization with dextran as sacrificial template allows better entrapment of cellulase within the material, improving loading capacity and stability of immobilized cellulase [ 58 ]. Mesoporous Zn-based MOFs were also utilized for cellulase encapsulation by simultaneous precipitation of the MOF precursors and cellulase ( Figure 2 ) [ 59 ].…”

Section: Methods Of Cellulase Immobilizationmentioning

confidence: 99%

Cellulase Immobilization on Nanostructured Supports for Biomass Waste Processing

2022

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Nanobiocatalysts, i.e., enzymes immobilized on nanostructured supports, received considerable attention because they are potential remedies to overcome shortcomings of traditional biocatalysts, such as low efficiency of mass transfer, instability during catalytic reactions, and possible deactivation. In this short review, we will analyze major aspects of immobilization of cellulase—an enzyme for cellulosic biomass waste processing—on nanostructured supports. Such supports provide high surface areas, increased enzyme loading, and a beneficial environment to enhance cellulase performance and its stability, leading to nanobiocatalysts for obtaining biofuels and value-added chemicals. Here, we will discuss such nanostructured supports as carbon nanotubes, polymer nanoparticles (NPs), nanohydrogels, nanofibers, silica NPs, hierarchical porous materials, magnetic NPs and their nanohybrids, based on publications of the last five years. The use of magnetic NPs is especially favorable due to easy separation and the nanobiocatalyst recovery for a repeated use. This review will discuss methods for cellulase immobilization, morphology of nanostructured supports, multienzyme systems as well as factors influencing the enzyme activity to achieve the highest conversion of cellulosic biowaste into fermentable sugars. We believe this review will allow for an enhanced understanding of such nanobiocatalysts and processes, allowing for the best solutions to major problems of sustainable biorefinery.

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Section: Methods Of Cellulase Immobilizationmentioning

confidence: 99%

Cellulase Immobilization on Nanostructured Supports for Biomass Waste Processing

2022

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“…Mesoporous zirconium-based MOF (MP-UIO-66) has been reported to be capable of deimmobilization of cellulase. [131] MP-UIO-66 has more mesoporous channels (4.6 nm) to encapsulate cellulase, which improves the loading capacity (265 mg/g) and stability of the immobilized enzyme. After 6 cycles, the load is 83%.…”

Section: Metal-organic Framework (Mof)mentioning

confidence: 99%

Research Progress on Lignocellulosic Biomass Degradation Catalyzed by Enzymatic Nanomaterials

Luo

Liu

et al. 2022

Chemistry An Asian Journal

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Lignocellulose biomass (LCB) has extensive applications in many fields such as bioenergy, food, medicines, and raw materials for producing value-added products. One of the keys to efficient utilization of LCB is to obtain directly available oligo-and monomers (e. g., glucose). With the characteristics of easy recovery and separation, high efficiency, economy, and environmental protection, immobilized enzymes have been developed as heterogeneous catalysts to degrade LCB effectively. In this review, applications and mechanisms of LCB-degrading enzymes are discussed, and the nanomaterials and methods used to immobilize enzymes are also discussed. Finally, the research progress of lignocellulose biodegradation catalyzed by nano-enzymes was discussed.

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Fabrication of Thermo‐Responsive Polymer‐MOF@cellulase Composites with Improved Catalytic Performance for Hydrolysis of Cellulose

Ali Tajwar,

Liu,

2024

Chemistry An Asian Journal

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Metal‐organic frameworks (MOFs) are considered as an ideal enzyme support because of their porous structural superiority. However, MOFs@enzyme composites have usually compromised their hydrolysis efficiency due to the narrow space inducing unfavourable enzyme conformations. Herein, a thermo‐responsive poly(N,N‐dimethylacrylamide) (PD) was fixed onto the surface of UiO‐66‐NH2 (UiO) through a post‐synthetic modification protocol. Using poly(2‐vinyl‐4,4 dimethylazlactone) (V) as a linker, PVD‐UiO@cellulase composites were fabricated after cellulase was immobilized onto the UiO surface through covalent bonding. The composites conferred favorable cellulose conformations, boosting hydrolysis efficiency and stability, which relied on the soft PVD shell and confinement effect yielded by the curled PVD chains at high temperatures. Compared with free cellulase, the proposed composites exhibited a 33.1‐fold enhancement of the Kcat values at 50 °C. The PVD‐UiO@cellulase composites were applied to the hydrolysis of cellulose in the stalks and leaves of Epipremnum aureum. The results highlight the potential of smart PVD‐UiO@cellulase composites in the hydrolysis of cellulose, affording a valuable platform for the preparation of unique MOFs@enzyme composites and their industrial applications.

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A method of preparing mesoporous Zr-based MOF and application in enhancing immobilization of cellulase on carrier surface

Cited by 17 publications

References 55 publications

Cellulase Immobilization on Nanostructured Supports for Biomass Waste Processing

Cellulase Immobilization on Nanostructured Supports for Biomass Waste Processing

Research Progress on Lignocellulosic Biomass Degradation Catalyzed by Enzymatic Nanomaterials

Fabrication of Thermo‐Responsive Polymer‐MOF@cellulase Composites with Improved Catalytic Performance for Hydrolysis of Cellulose

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