A Method of Producing Carcinoma in Upper Aerodigestive Tree and Esophagus of the Syrian Golden Hamster Using Wounding and Instillation of N-Methylnitrosourea

Abstract

Details of a method for producing carcinoma of the aerodigestive tree of the Syrian golden hamster and the use of this model to evaluate putative agents for chemoprevention of these carcinomas are described. The method produces a majority of squamous carcinomas of the trachea and glottis that follow squamous metaplasia of respiratory epithelium. In addition, seen are adenocarcinomas arising in glands of the respiratory tree. Squamous carcinomas of the digestive epithelium arise in primary squamous epithelium. These tumors of digestive epithelium have a growth pattern that differs from that of the respiratory epithelium in that they grow and invade without filling the epithelial layer with tumor cells. (Cancer Epidemiol Biomarkers Prev 2007;16(8):1644 -50)

The Experimental Use of Syrian Hamsters

“…The hamster is susceptible to tumors of the upper airways. These tumors are induced by inhalation or topical application of carcinogens with or without the addition of co-carcinogenic stimuli (Estensen et al, 2007;Homburger et al, 1979;Moon et al, 1992aMoon et al, , 1992b. A reported limitation of topical carcinogen application models is the lack of reproducibility among different labs (Estensen et al, 2007).…”

Acute hematologic, hepatologic, and nephrologic changes after intraperitoneal injections of 18 nm gold nanoparticles in hamsters

“…29 The pattern of the upper aerodigestive tree, and esophageal cancers produced in hamsters resembles that seen in human smokers and suggests that this model may serve as a system for testing chemopreventive or chemotherapeutic agents for tumors in these areas. 30 As far as we know, no quantitative or semiquantitative biodistribution study of the 18 nm GNPs after in vivo I/P administration in hamsters exists. Thereby, the aim of this study was to investigate the effects of repeated I/P injection of 18 nm sized GNPs on some hematologic parameters, on the hepatic and renal functions and on the histopathology of solid organs in healthy adult male Syrian golden hamsters receiving 30 ppb of GNPs daily for 14 consecutive days (a total dose of 420 ppb per animal).…”

N‐methylnitrosourea–induced carcinoma as a model for laryngeal carcinogenesis

“…The wounding and administration of MNU is described briefly in this article, and in more detail previously . Mice were anesthetized with Avertin (2,2,2 Tribromoethanol) at 225 to 240 mg/kg intraperitoneal.…”

“…Histology evaluation showed features consistent with human carcinomas, including ulceration, lymphatic spread, and encroachment of the airway . Establishment of upper respiratory tract carcinoma after MNU treatment has been described in hamsters . Transgenic mice have been used in combination with MNU treatment to study sarcomas, lymphomas, and gastric and mammary carcinogenesis, but not in laryngeal carcinoma.…”