A method of screening test for excretion pattern of urinary glycosaminoglycans and its application to normal human urine.

Excretion pattern of urinary glycosaminoglycans from orthopedic patients.

“…However, band 1 often spread over band 2 zone as reported previously (Nagatsuka et al 1980). In these cases, determination of hexosamine was made as bands 1+2.…”

“…All the urines so far examined gave three regular GAG-bands, which were designated as bands 1, 2 and 3 as reported previously (Nagatsuka et al 1980). However, band 1 often spread over band 2 zone as reported previously (Nagatsuka et al 1980).…”

“…Pronase P, Separax (cellulose acetate membrane), Toluidine blue, Alcian blue 8GS, cetylpyridinium chloride and other materials were those described previously (Nagatsuka et al 1980). …”

“…Separation of crude GAG from urine, quantitation of GAG by Separax electrophoresis and quantitation of hexosamine were performed as described previously (Nagatsuka et al 1980). …”

“…To obtain a clue for the metabolic disorder of GAG in other connective tissue diseases, a device of screening test for excretion pattern of urinary GAG was achieved in a previous paper (Nagatsuka et al 1980). This paper reports the results of an application of the screening test to urines from orthopedic patients.…”