A Method of Solubilizing and Concentrating Astaxanthin and Other Carotenoids

Hara, Kiyotaka Y.; Yagi, Shuwa; Hirono-Hara, Yoko; Kikukawa, Hiroshi

doi:10.3390/md19080462

Cited by 9 publications

(7 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The biological availability of AST is limited due to its low aqueous solubility [ 23 , 33 ]. β-cyclodextrin (bCD) solubilized AST 110-fold and thus increased antioxidant activity by up to 7-fold compared to free AST [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, low aqueous solubility (less than 2 µg/mL in pH 6.5 solution) and low stability are also problematic for practical application in humans [ 20 , 21 ]. To solve the disadvantages and problems of AST, various formulations based on drug delivery systems have been investigated [ 22 , 23 , 24 ]. Polyakov and Kispert reported that inclusion complexes based on polysaccharides increase aqueous solubility and photostability [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Anticancer Activity of Astaxanthin-Incorporated Chitosan Nanoparticles

Hwang,

Jeong,

Lee

et al. 2024

Molecules

View full text Add to dashboard Cite

Astaxanthin (AST)-encapsulated nanoparticles were fabricated using glycol chitosan (Chito) through electrostatic interaction (abbreviated as ChitoAST) to solve the aqueous solubility of astaxanthin and improve its biological activity. AST was dissolved in organic solvents and then mixed with chitosan solution, followed by a dialysis procedure. All formulations of ChitoAST nanoparticles showed small diameters (less than 400 nm) with monomodal distributions. Analysis with Fourier transform infrared (FT-IR) spectroscopy confirmed the specific peaks of AST and Chito. Furthermore, ChitoAST nanoparticles were formed through electrostatic interactions between Chito and AST. In addition, ChitoAST nanoparticles showed superior antioxidant activity, as good as AST itself; the half maximal radical scavenging concentrations (RC50) of AST and ChitoAST nanoparticles were 11.8 and 29.3 µg/mL, respectively. In vitro, AST and ChitoAST nanoparticles at 10 and 20 µg/mL properly inhibited the production of intracellular reactive oxygen species (ROSs), nitric oxide (NO), and inducible nitric oxide synthase (iNOS). ChitoAST nanoparticles had no significant cytotoxicity against RAW264.7 cells or B16F10 melanoma cells, whereas AST and ChitoAST nanoparticles inhibited the growth of cancer cells. Furthermore, AST itself and ChitoAST nanoparticles (20 µg/mL) efficiently inhibited the migration of cancer cells in a wound healing assay. An in vivo study using mice and a pulmonary metastasis model showed that ChitoAST nanoparticles were efficiently delivered to a lung with B16F10 cell metastasis; i.e., fluorescence intensity in the lung was significantly higher than in other organs. We suggest that ChitoAST nanoparticles are promising candidates for antioxidative and anticancer therapies of B16F10 cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Anticancer Activity of Astaxanthin-Incorporated Chitosan Nanoparticles

Hwang,

Jeong,

Lee

et al. 2024

Molecules

View full text Add to dashboard Cite

show abstract

“…Consumers prefer to buy shrimp that are light grey in color and turn an intense orange color when cooked [61]. The color of shrimp is due to the accumulation of crustacyanin, which is formed by a protein-astaxanthin complex [62]. During cooking, color changes in crustaceans are closely related to the dissociation of astaxanthin (3,3dihydroxy-β,β'-carotene-4,4 -dione) from its pigment proteins [63].…”

Section: Discussionmentioning

confidence: 99%

Effects of including of Japanese Pumpkin Seeds and Pomace in the Diets of Pacific White Shrimp (Penaeus vannamei)

Zancan,

Monserrat,

Marreiro Gomes

et al. 2023

Animals

View full text Add to dashboard Cite

A 60-day feeding trial was conducted to evaluate the effects of including pumpkin seeds and pomace in the diets of Pacific white shrimp Penaeus vannamei, and the effects of these supplements on growth performance, body composition, and total polyphenol, flavonoid and carotenoid contents, as well as on total antioxidant activity, and body color parameters. Five diets were evaluated: pumpkin seeds (PS) at 50 and 100 g·kg−1, pumpkin pomace (PP) at 50 and 100 g·kg−1, and a control treatment. Pacific white shrimp (P. vannamei) juveniles (0.60 ± 0.01 g) were stocked in 15 tanks (310 L), containing 30 shrimps per tank, and the treatments were randomly distributed in triplicate. At the end of the experiment, shrimps were euthanized, weighed, and dissected for further analyses. The inclusion of PS in the diets impaired growth performance, reduced the total flavonoid content and had a pro-oxidative effect on muscle. The inclusion of PP in the diets did not affect growth performance, improved the feed conversion ratio, increased the total flavonoid content in the diets and hepatopancreas, and improved the antioxidant activity of the feeds and shrimp muscle. The total carotenoid content of the feeds increased with the inclusion of PS or PP in the diets; however, the total carotenoid content of shrimp increased only in those fed PP diets. Shrimp fed with PS diets showed a yellowish color and higher saturation when fresh and a reddish color and yellow hue angle after cooking. Shrimp fed PP diets turned reddish and yellowish, both when fresh and after cooking. The inclusion of PS in P. vannamei diets is not recommended; however, PP can be included at 100 g·kg−1 without affecting the growth parameters. Further studies evaluating the inclusion of higher PP levels in shrimp diets are recommended.

show abstract

“…En zanahoria naranja, se encontró que el gen CYP97A3 es responsable de los altos niveles de acumulación de α-caroteno (Ren et al, 2022). Un valioso carotenoide, la astaxantina (3, 30dihidroxi-β, β-caroteno-4, 40-diona; C40H52O4), actúa como agente protector frente al daño oxidativo de células in vivo porque tiene una alta actividad antioxidante (Hara et al, 2021).…”

Section: β-Caroteno Y Astaxantinaunclassified

Carotenoides

Romero,

Espinoza

2024

View full text Add to dashboard Cite

Con gran satisfacción y entusiasmo se presenta ante la comunidad científica, la presente obra, un libro dedicado a despejar las innumerables dudas respecto al conocimiento de las características, propiedades y métodos de extracción de carotenoides: carotenos y xantófilas. Este libro enfatiza en la importancia que tiene conocer acerca de la estructura, propiedades, biosíntesis, biodisponibilidad en el cuerpo humano, factores que afectan su biodisponibilidad y técnicas de extracción de los carotenoides es para conocer el fundamento de sus funciones esenciales tanto en las plantas como en el ser vivo, en plantas, captan la energía luminosa y actúan como antioxidantes. En el ser vivo al consumir los carotenoides se transforman en vitamina A, micronutriente que interviene en una amplia variedad de funciones fisiológicas, entre las que destaca la función visual, formación y mantenimiento de tejidos blandos, dientes, mucosas y piel, y tiene también un papel clave en el desarrollo fetal, embarazo y lactancia.

show abstract

A Method of Solubilizing and Concentrating Astaxanthin and Other Carotenoids

Cited by 9 publications

References 24 publications

Anticancer Activity of Astaxanthin-Incorporated Chitosan Nanoparticles

Anticancer Activity of Astaxanthin-Incorporated Chitosan Nanoparticles

Effects of including of Japanese Pumpkin Seeds and Pomace in the Diets of Pacific White Shrimp (Penaeus vannamei)

Carotenoides

Contact Info

Product

Resources

About