A Method to Create Golden Hull Rice Biological Resources Using CRISPR/Cas9 System

Zhi-qun, Que; Lu, Qianyi; Liu, Xiaolin; Li, Shengqiang; Zeng, Huilan; Li, Rungen; Liu, Tao; Yan, Xiaoyu; Shen, Chunxiu

doi:10.1166/jbmb.2018.1750

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“…Striking examples are that of plant color that has been obtained by gene editing of anthocyanin biosynthetic genes such as chalcone isomerase (CHI) in rice (Oryza sativa), flavanone-3-hydroxylase (F3H) in carrots (Daucus carota) and dihydrofavonol-4-reductase-B (DFR-B) in Japanese morning glories (Ipomoea nil). These genes were modified to produce mutants with golden hulls [6], purple-colored calluses [7] and white flowers [8], respectively. Carotenoid biosynthetic genes, like carotenoid isomerase (CRTISO), phytoene synthase 1 (PSY1) and beta-carotene hydroxylase 2 (CrtR-b2) in tomatoes, have also been edited using the CRISPR/Cas9 system to yield a series of novel variations [9,10].…”

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confidence: 99%

Transcriptome Profiling of Ornithogalum dubium Leaves and Flowers to Identify Key Carotenoid Genes for CRISPR Gene Editing

Wei

Arazi

Hod

et al. 2020

Plants

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Ornithogalum dubium is a popular ornamental monocot native to South Africa with flower colors ranging from pure white to deep orange. Gene editing based on the CRISPR/Cas9 system has recently been shown to hold potential for color improvement in ornamental flower crops. To apply this approach to Ornithogalum color manipulation, genomic or transcriptomic data must first be collected. Here, cDNA libraries of O. dubium leaves and flowers were constructed and sequenced using the Illumina HiSeq 2500. Over 155 million 100-bp paired-end reads were assembled into a transcriptome database of 360,689 contigs, of which 18,660 contigs were differentially expressed between leaves and flowers. Carotenoids are the main pigment imparting spectrum of orange hues to O. dubium flowers. By querying our database, we identified a total of 16 unique transcripts (unigenes) predicted to be involved in the carotenoid biosynthesis pathway of Ornithogalum. Combining carotenoid profiles, we further inferred several key unigenes responsible for floral coloration and accumulation in O. dubium, of which the gene LCYB/comp146645_c0 was found as a suitable target to generate potentially red flower varieties of O. dubium. Our research thus provides a framework for the application of CRISPR/Cas9 technology to improve this ornamental crop.

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