A method to perform direct transcutaneous intrathecal injection in rats

Mestre, Christine; Pélissier, Teresa; Fialip, J.; Wilcox, George L.; Eschalier, Alain

doi:10.1016/1056-8719(94)90087-6

Cited by 466 publications

(261 citation statements)

References 7 publications

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“…Seven days following vector inoculation, rats were injected with 20 nmol of naltrexone (Sigma) in sterile 0.9% saline via direct transcutaneous injection between the dorsal aspects of L5 and L6. 45 Five minutes later, nocifensive behavior was evaluated using the formalin test.…”

Section: Intrathecal Injectionmentioning

confidence: 99%

Antinociceptive effect of a genomic herpes simplex virus-based vector expressing human proenkephalin in rat dorsal root ganglion

et al. 2001

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Endogenous opiate peptides acting pre-and post-synaptically in the dorsal horn of spinal cord inhibit transmission of nociceptive stimuli. We transfected neurons of the dorsal root ganglion in vivo by footpad inoculation with 30 l (3 × 10 7 p.f.u.) of a replication-incompetent (ICP4-deleted) herpes simplex virus (HSV) vector with a cassette containing a portion of the human proenkephalin gene coding for 5 metand 1 leu-enkephalin molecules under the control of the human cytomegalovirus immediate-early promoter (HCMV IEp) inserted in the HSV thymidine kinase (tk) locus. Vectordirected expression of enkephalin produced a significant

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Section: Intrathecal Injectionmentioning

confidence: 99%

Antinociceptive effect of a genomic herpes simplex virus-based vector expressing human proenkephalin in rat dorsal root ganglion

et al. 2001

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“…To study the effect of 5-HT 2 R blockade on ultra-low-dose buprenorphine-induced hyperalgesia, the 5-HT 2A R, 5-HT 2B R, and 5-HT 2C R antagonists 4F 4PP (2 mM), SB 204741 (1 mM), and RS 102221 (1 M, all in 25% DMSO/NaCl) or saline were injected intrathecally 15 min before ultra-low-dose buprenorphine injection. Transcutaneous intrathecal injections were modified from Mestre et al (1994). Briefly, animals were anesthetized with isoflurane.…”

Section: Methodsmentioning

confidence: 99%

Pronociceptive and Antinociceptive Effects of Buprenorphine in the Spinal Cord Dorsal Horn Cover a Dose Range of Four Orders of Magnitude

Gerhold

Drdla-Schutting

Honsek

et al. 2015

Journal of Neuroscience

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Due to its distinct pharmacological profile and lower incidence of adverse events compared with other opioids, buprenorphine is considered a safe option for pain and substitution therapy. However, despite its wide clinical use, little is known about the synaptic effects of buprenorphine in nociceptive pathways. Here, we demonstrate dose-dependent, bimodal effects of buprenorphine on transmission at C-fiber synapses in rat spinal cord dorsal horn in vivo. At an analgesically active dose of 1500 g⅐kg Ϫ1, buprenorphine reduced the strength of spinal C-fiber synapses. This depression required activation of spinal opioid receptors, putatively 1 -opioid receptors, as indicated by its sensitivity to spinal naloxone and to the selective 1 -opioid receptor antagonist naloxonazine. In contrast, a 15,000-fold lower dose of buprenorphine (0.1 g⅐kg Ϫ1 ), which caused thermal and mechanical hyperalgesia in behaving animals, induced an enhancement of transmission at spinal C-fiber synapses. The ultra-low-dose buprenorphine-induced synaptic facilitation was mediated by supraspinal naloxonazine-insensitive, but CTOP-sensitive -opioid receptors, descending serotonergic pathways, and activation of spinal glial cells. Selective inhibition of spinal 5-hydroxytryptamine-2 receptors (5-HT 2 Rs), putatively located on spinal astrocytes, abolished both the induction of synaptic facilitation and the hyperalgesia elicited by ultra-low-dose buprenorphine. Our study revealed that buprenorphine mediates its modulatory effects on transmission at spinal C-fiber synapses by dose dependently acting on distinct -opioid receptor subtypes located at different levels of the neuraxis.

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“…injection. Injections were performed as previously described (50). For ascertaining the areas in the brain ventricular system into which the drugs penetrated, the i.c.v.…”

Section: Methodsmentioning

confidence: 99%

Central serotonergic neurons are differentially required for opioid analgesia but not for morphine tolerance or morphine reward

Zhao¹,

Gao²,

Sun³

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

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Opioids remain the most effective analgesics despite their potential adverse effects such as tolerance and addiction. Mechanisms underlying these opiate-mediated processes remain the subject of much debate. Here we describe opioid-induced behaviors of Lmx1b conditional knockout mice (Lmx1b f/f/p ), which lack central serotonergic neurons, and we report that opioid analgesia is differentially dependent on the central serotonergic system. Analgesia induced by a opioid receptor agonist administered at the supraspinal level was abolished in Lmx1b f/f/p mice compared with their wild-type littermates. Furthermore, compared with their wild-type littermates Lmx1b f/f/p mice exhibited significantly reduced analgesic effects of and ␦ opioid receptor agonists at both spinal and supraspinal sites. In contrast to the attenuation in opioid analgesia, Lmx1b f/f/p mice developed tolerance to morphine analgesia and displayed normal morphine reward behavior as measured by conditioned place preference. Our results provide genetic evidence supporting the view that the central serotonergic system is a key component of supraspinal pain modulatory circuitry mediating opioid analgesia. Furthermore, our data suggest that the mechanisms of morphine tolerance and morphine reward are independent of the central serotonergic system. serotonin ͉ pain ͉ conditioned place preference ͉ behavior ͉ mouse

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A method to perform direct transcutaneous intrathecal injection in rats

Cited by 466 publications

References 7 publications

Antinociceptive effect of a genomic herpes simplex virus-based vector expressing human proenkephalin in rat dorsal root ganglion

Antinociceptive effect of a genomic herpes simplex virus-based vector expressing human proenkephalin in rat dorsal root ganglion

Pronociceptive and Antinociceptive Effects of Buprenorphine in the Spinal Cord Dorsal Horn Cover a Dose Range of Four Orders of Magnitude

Central serotonergic neurons are differentially required for opioid analgesia but not for morphine tolerance or morphine reward

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