A method to quantify viable carbapenem resistant gram-negative bacteria in treated and untreated wastewater

Reinke, Ryan A.; Quach-Cu, Jennipher; Allison, Nigel; Lynch, Bellanira; Crisóstomo, C.M.; Padilla, M. J.

doi:10.1016/j.mimet.2020.106070

Cited by 9 publications

(7 citation statements)

References 41 publications

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“…Also, the loss of plasmids between phenotypic resistance testing and the sequencing of the isolates cannot be fully excluded. Other studies demonstrated an increased load of multiple resistant 3GCREC and even carbapenem-resistant Enterobacterales (CRE) in WWTPs [6,43,44]. However, carbapenemase genes could not be detected in any isolate used in our study.…”

Section: Discussioncontrasting

confidence: 64%

High Genetic Diversity in Third-Generation Cephalosporin-Resistant Escherichia coli in Wastewater Systems of Schleswig-Holstein

Carlsen,

Grottker,

Heim

et al. 2024

Pathogens

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The spread of multidrug-resistant bacteria from humans or livestock is a critical issue. However, the epidemiology of resistant pathogens across wastewater pathways is poorly understood. Therefore, we performed a detailed comparison of third-generation cephalosporin-resistant Escherichia coli (3GCREC) from wastewater treatment plants (WWTPs) to analyze dissemination pathways. A total of 172 3GCREC isolated from four WWTPs were characterized via whole genome sequencing. Clonal relatedness was determined using multi-locus sequence typing (MLST) and core genome MLST. Resistance genotypes and plasmid replicons were determined. A total of 68 MLST sequence types were observed with 28 closely related clusters. Resistance genes to eight antibiotic classes were detected. In fluoroquinolone-resistant isolates, resistance was associated with three-or-more point mutations in target genes. Typing revealed high genetic diversity with only a few clonal lineages present in all WWTPs. The distribution paths of individual lines could only be traced in exceptional cases with a lack of enrichment of certain lineages. Varying resistance genes and plasmids, as well as fluoroquinolone resistance-associated point mutations in individual isolates, further corroborated the high diversity of 3GCREC in WWTPs. In total, we observed high diversity of 3GCREC inside the tested WWTPs with proof of resistant strains being released into the environment even after treatment processes.

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Section: Discussioncontrasting

confidence: 64%

High Genetic Diversity in Third-Generation Cephalosporin-Resistant Escherichia coli in Wastewater Systems of Schleswig-Holstein

Carlsen,

Grottker,

Heim

et al. 2024

Pathogens

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“…Acquisition of MDR genes occurs most commonly in hospitals and chronic care facilities, but community-acquired sources including wild and domestic animals, food products, and environmental contamination may drive resistance 5,10,50,93,94 . Spread of MDR genes has also been linked to community and hospital effluent treatment systems 95,96 . Movement of humans, wild or domestic animals, livestock and food products is a key factor in spreading MDR in the environment 93,94,[97][98][99] .…”

Section: International Dissemination Of Antimicrobial Resistant Genetic Elementsmentioning

confidence: 99%

Escalating antimicrobial resistance among Enterobacteriaceae: focus on carbapenemases

Lynch¹,

Clark

Zhanel

2021

Expert Opinion on Pharmacotherapy

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aminoglycosides (AG); antimicrobial resistance (AMR); -lactamase inhibitors (BLI); blood stream infections (BSIs) ; carbapenem (CP); carbapenemase (CPase); carbapenem-resistant (CR); carbapenemresistant Enterobacterales (CRE) ;-lactam inhibitor (BLI); CP-resistant K. pneumoniae (CRKP); CPsusceptible K. pneumoniae (CSKP); ceftazidime/avibactam (CAZ-AVI); cephalosporins (CEPHS); central line-associated (CLA); central venous catheter (CVC); community-acquired infection (CAI); complicated intra-abdominal infection (c-IAI); complicated urinary tract infection (c-UTI); defined daily dose (DDD) ; extended-spectrum -lactamases (ESBLs); extensively Drug Resistant (eXDR); fluoroquinolones (FQ); Gram negative bacteria (GNB); gross domestic product per capita (GDPPC); high-income countries (HICs); hospital acquired infection (HAI); imipenem-relebactam (IMI-REL); intensive care units (ICUs); Klebsiella pneumoniae carbapenemases (KPCs); long-term care facilities (LTCFs); low-and middleincome countries (LMICs); mechanical ventilation; metallo-lactamase (MBL); minimal inhibitory concentration (MIC); mobile genetic elements (MGEs); multidrug resistant (MDR); New Delhi Metalloproteinase-1 (NDM-1); non-Proteus Enterobacterales (NPE); organ transplant recipients (OTR); piperacillin/tazobactam (PTZ); solid organ transplant (SOT); tigecycline (TGC); ventilator-associated pneumonia (VAP)

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“…Although the bacterial numbers were high in the raw wastewater samples, the ratios of presumptive CRE to total Enterobacterales were below 0.02%. The ratio of CRE numbers to the heterotrophic plate count numbers was reported to be approximately 10 −4 (0.01%) to 10 −5 (0.001%) for wastewater samples in previous studies [ 10 , 18 ]. In our previous study, the ratios of presumptive CRE were observed between 0.014 and 0.31% using slightly different isolation methods from those in this study [ 20 ].…”

Section: Resultsmentioning

confidence: 96%

“…The concentration of meropenem added to the medium was set at 0.5 μg/mL in this study, which was lower than the breakpoints provided by CLSI and EUCAST. The reason for the lower concentration was to select more presumptive colonies based on our previous results [ 20 ] so that CRE would be selected by the confirmation test from the presumptive colonies [ 10 ].…”

Section: Methodsmentioning

confidence: 99%

“…A study of U.S. wastewater showed that 65 out of 322 Escherichia coli isolates were imipenem-resistant [ 8 ], but the reported incidence of CRE was kept in a low range of 0.3–2.93 infections per 100,000 person-years across the entire U.S. population [ 9 ]. Although a high removal (>99.9%) of CRE from wastewater was reported in the treatment processes [ 10 ], the treatment plants are considered to be hotspots for resistant bacteria in the environment [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

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Monitoring Carbapenem-Resistant Enterobacterales in the Environment to Assess the Spread in the Community

2022

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The usefulness of wastewater-based epidemiology (WBE) was proven during the COVID-19 pandemic, and the role of environmental monitoring of emerging infectious diseases has been recognized. In this study, the prevalence of carbapenem-resistant Enterobacterales (CRE) in Japanese environmental samples was measured in the context of applying WBE to CRE. A total of 247 carbapenem-resistant isolates were obtained from wastewater, treated wastewater, and river water. Treated wastewater was shown to be an efficient target for monitoring CRE. The results of the isolate analysis showed that WBE may be applicable to Escherichia coli-carrying New Delhi metallo-β-lactamase (NDM)-type carbapenemase, the Enterobacter cloacae complex and Klebsiella pneumoniae complex-carrying IMP-type carbapenemase. In addition, a certain number of CRE isolated in this study carried Guiana extended spectrum (GES)-type carbapenemase although their clinical importance was unclear. Only a few isolates of Klebsiella aerogenes were obtained from environmental samples in spite of their frequent detection in clinical isolates. Neither the KPC-type, the oxacillinase (OXA)-type nor the VIM-type of carbapenemase was detected in the CRE, which reflected a low regional prevalence. These results indicated the expectation and the limitation of applying WBE to CRE.

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A method to quantify viable carbapenem resistant gram-negative bacteria in treated and untreated wastewater

Cited by 9 publications

References 41 publications

High Genetic Diversity in Third-Generation Cephalosporin-Resistant Escherichia coli in Wastewater Systems of Schleswig-Holstein

High Genetic Diversity in Third-Generation Cephalosporin-Resistant Escherichia coli in Wastewater Systems of Schleswig-Holstein

Escalating antimicrobial resistance among Enterobacteriaceae: focus on carbapenemases

Monitoring Carbapenem-Resistant Enterobacterales in the Environment to Assess the Spread in the Community

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