Background: In Ethiopia, brucellosis has been reported targeted on bovine, occasionally on shoat, and rarely on camels. An investigation of the disease Brucellosis in the neglected companion animals is scared in Ethiopia. The objective of this study was to identify canine brucellosis in Batu town, Alage and Naka village through cross sectional approaches. A total of 389 serum samples (207 from Batu, 107 from Alage, and 75 from Naka) were collected by restraining dogs with a portable and safe modified dog crush, invented by this author. Blood samples were collected from ear vein and sera were screened for Brucella antibodies using different serological tests. RBPT prepared from the smooth strain B. abortus antigen and CFT was used as a screening test and confirmatory test, respectively. Furthermore, all sera samples had also screened by RBPTcanis antigen (rough strain); and those positive were considered the cause for B. canis infection. Results: Using RBPT smooth strains, 21(5.4%; CI: 3.35, 7.96) were positive and 19(4.88%; CI: 2.7, 7.0) were confirmed by CFT. Besides, 34 (8.74%; CI: 5.92, 11.56) were positive for RBPTcanis rough strains. Relatively, higher proportion of anti B. canis antibodies had seen in Batu (11.59%) followed by Alage (5.61%), and Naka (5.33%). Sex, living condition, and history of obstetrical problem were significantly associated with the occurrence of canine brucellosis (p< 0.05). Odd of canine brucellosis due to smooth and rough strains in outdoor dogs were 4.72 and 6.42 times higher compared with indoors, respectively. This is true the fact that outdoors had a chance of getting infected aborted wastes when roaming freely. Conclusion: This study suggests that canine brucellosis is prevalent in the province. The seropositivity could give an insight that, the awareness of the people toward the disease was also the gap in the study area. Hence, this warrants public education among the community is recommended.