A methylene blue and Ag+ ratiometric electrochemical aptasensor based on Au@Pt/Fe-N-C signal amplification strategy for zearalenone detection

Suo, Zhiguang; Niu, Xingyuan; Liu, Ruike; Xin, Lingkun; Liu, Yong; Wei, Min

doi:10.1016/j.snb.2022.131825

Cited by 29 publications

(3 citation statements)

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“…At the same time, methylene blue (MB), a commonly used cationic redox probe (p K 1 = 11.2), was used as the electrochemical probe. ( Zhang et al, 2022b ; Liu et al, 2022 ; Prado et al, 2022 ; Suo et al, 2022 ). Since the redox peaks of MB are at a negative potential, the electrochemical interference of redox small molecules (for example, ascorbic acid-AA, uric acid-UA, dopamine-DA, tryptophan, etc) in biological samples (for example, serum) can be effectively avoided because of their positive redox potentials.…”

Section: Resultsmentioning

confidence: 99%

A reagentless electrochemical immunosensor for sensitive detection of carcinoembryonic antigen based on the interface with redox probe-modified electron transfer wires and effectively immobilized antibody

et al. 2022

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Convenient and sensitive detection of tumors marked in serum samples is of great significance for the early diagnosis of cancers. Facile fabrication of reagentless electrochemical immunosensor with efficient sensing interface and high sensitivity is still a challenge. Herein, an electrochemical immunosensor was easily fabricated based on the easy fabrication of immunoassay interface with electron transfer wires, confined redox probes, and conveniently immobilized antibodies, which can achieve sensitive and reagentless determination of the tumor marker, carcinoembryonic antigen (CEA). Carboxyl multi-walled carbon nanotubes (MWCNTs) were firstly modified with an electrochemical redox probe, methylene blue (MB), which has redox potentials distinguished from those of redox molecules commonly existing in biological samples (for example, ascorbic acid and uric acid). After the as-prepared MB-modified MWCNT (MWCNT-MB) was coated on the supporting glassy carbon electrode (GCE), the MWCNT-MB/GCE exhibited improved active area and electron transfer property. Polydopamine (PDA) was then in situ synthesized through simple self-polymerization of dopamine, which acts as the bio-linker to covalently immobilize the anti-CEA antibody (Ab). The developed immunosensor could be applied for electrochemical detection of CEA based on the decrease in the redox signal of MB after specific binding of CEA and immobilized Ab. The fabricated immunosensor can achieve sensitive determination of CEA ranging from 10 pg/ml to 100 ng/ml with a limit of detection (LOD) of 0.6 pg/ml. Determination of CEA in human serum samples was also realized with high accuracy.

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Section: Resultsmentioning

confidence: 99%

A reagentless electrochemical immunosensor for sensitive detection of carcinoembryonic antigen based on the interface with redox probe-modified electron transfer wires and effectively immobilized antibody

et al. 2022

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“…However, as compared to other rapid analytical methods for ZEN, our chemiluminescence aptasensor is satisfactory in both detection range and limit. Even though some analytical methods based on electrochemical [55], fluorescent [56,57], colorimetric [58] and chemiluminescent [59] signal transduction display ultra-sensitivity of below 1 ng/mL, their detection range is much narrower as compared to our developed aptasensor (Table 1). As can also be noticed, our aptasensor is comparable in both detection range and LOD to ELISA [60], AuNPs-based lateral flow assay [52] and colorimetry [61].…”

Section: Analytical Performancementioning

confidence: 96%

A Novel and Label-Free Chemiluminescence Detection of Zearalenone Based on a Truncated Aptamer Conjugated with a G-Quadruplex DNAzyme

Guan

Neng

et al. 2023

Biosensors

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Zearalenone (ZEN), one of the most frequently occurring mycotoxin contaminants in foods and feeds, poses considerable threat to human and animal health, owing to its acute and chronic toxicities. Thus, rapid and accurate detection of ZEN has attracted broad research interest. In this work, a novel and label-free chemiluminescence aptasensor based on a ZEN aptamer and a G-quadruplex DNAzyme was constructed. It was established on a competitive assay between ZEN and an auxiliary DNA for the aptamer, leading to activation of the G-quadruplex/hemin DNAzyme and subsequent signal amplification by chemiluminescence generation after substrate addition. To maximize the detection sensitivity, numerous key parameters including truncated aptamers were optimized with molecular docking analysis. Upon optimization, our aptasensor exhibited a perfect linear relationship (R2 = 0.9996) for ZEN detection in a concentration range of 1–100 ng/mL (3.14–314.10 nM) within 40 min, achieving a detection limit of 2.85 ng/mL (8.95 nM), which was a 6.7-fold improvement over that before optimization. Most importantly, the aptasensor obtained a satisfactory recovery rate of 92.84–137.27% and 84.90–124.24% for ZEN-spiked wheat and maize samples, respectively. Overall, our label-free chemiluminescence aptasensor displayed simplicity, sensitivity, specificity and practicality in real samples, indicating high application prospects in the food supply chain for rapid detection of ZEN.

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“…Because of their superior self-calibration ability, the development of ratiometric electrochemical sensors has caused the increasing interest to offer the possible application in complicated test conditions. Currently, few metal ions like Bi(III) [16,26] and some redox species as ferrocene (Fc) [27][28][29], thionine (Th) [30], and methylene blue (MB) [31][32][33], are mainly employed as electrochemical signal indicators. Niu et al developed a stable ratiometric electrochemical sensor with the nitrogen doped bimetallic CoNi nanomaterials and MB was used as the internal reference, which realized for detecting p-phenylenediamine in commercial hair dyes [34].…”

Section: Introductionmentioning

confidence: 99%

A facile ratiometric electrochemical sensing platform with in‐situ electropolymerized thionine on effective sensing of 5‐hydroxytryptamine

Chen,

Xu,

Song

et al. 2023

Electroanalysis

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The ratiometric electrochemical sensors have been highly explored to minimize system errors and environmental interference. However, it is extremely desirable on the development of an effective ratiometric electrochemical sensor in a facile approach without the complicated assembly process. In this work, an effective internal reference probe was coupled based on the polymerized thionine (pTh) via a one‐step electrodeposition method. The ratiometric electrochemical sensing platform was applied for 5‐hydroxytryptamine (5‐HT) detection, where the internal reference probe, Th, presented the robust redox signal and can be clearly distinguished from the oxidation signal of 5‐HT. The proposed pTh modified GCE presented the outstanding electrochemical performance on sensing 5‐HT in a wide concentration range with a detection limit of 8.4 nΜ. Further, the fabricated sensor was efficiently employed for the detection of 5‐HT in various human serum samples and the obtained results were consistent with the reported range in serum samples. This work offers the great possibility on the fabrication of efficient ratiometric sensors in a facile approach and presents great application prospects in clinical diagnosis.

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A methylene blue and Ag+ ratiometric electrochemical aptasensor based on Au@Pt/Fe-N-C signal amplification strategy for zearalenone detection

Cited by 29 publications

References 50 publications

A reagentless electrochemical immunosensor for sensitive detection of carcinoembryonic antigen based on the interface with redox probe-modified electron transfer wires and effectively immobilized antibody

A reagentless electrochemical immunosensor for sensitive detection of carcinoembryonic antigen based on the interface with redox probe-modified electron transfer wires and effectively immobilized antibody

A Novel and Label-Free Chemiluminescence Detection of Zearalenone Based on a Truncated Aptamer Conjugated with a G-Quadruplex DNAzyme

A facile ratiometric electrochemical sensing platform with in‐situ electropolymerized thionine on effective sensing of 5‐hydroxytryptamine

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