A Micro-nano Melt Transcription Molding Process to produce Thermoplastic Devices with Tens Nanometers Scale Fine Patterns

Yakemoto, Kazutoshi; Itô, Hiroshi; Naito, Akihiro; Furuki, Kenichi

doi:10.2494/photopolymer.24.77

Cited by 1 publication

(3 citation statements)

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“…24,25) In other experiments, the activities of drug-metabolizing enzymes such as CYP1A2 and CYP3A4 of human hepatocytes in culture were found to be significantly higher in micro-space plates than in traditional plates. 13,26) In the present study, we also validated albumin secretion from hepatocytes to medium and the maintenance of mRNA expression levels of drugmetabolizing enzymes such as CYP1A2 and CYP3A4 in a novel primary three-dimensional culture system, suggesting that spheroid formation (three-dimensional formation of hepatocytes) mimics the cellular architecture found in native liver tissue.…”

Section: Resultsmentioning

confidence: 59%

“…13) In the present study, we evaluated a novel primary three-dimensional culture system using micro-space plates to determine the functional activity of human hepatocytes by measuring albumin secretion from hepatocytes to medium and measuring the expression levels of albumin, CYP1A2 and CYP3A4 mRNA. During cultivation, the cells weakly adhered to the surface of the micro-space plate and were not suspended in the culture medium.…”

Section: Resultsmentioning

confidence: 99%

“…3,[10][11][12] Recently, a micro-space plate was developed by Yakemoto et al as a tool for three-dimensional cell culture. 13) The micro-space plate has regularly arranged compartments on its surface as shown in Figure 1. Therefore, we attempted to maintain the mRNA expres- …”

Section: Introductionmentioning

confidence: 99%

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Secretion of Albumin and Induction of CYP1A2 and CYP3A4 in Novel Three-dimensional Culture System for Human Hepatocytes using Micro-space Plate

Nishimura¹,

Hagi²,

Ejiri³

et al. 2010

Drug Metabolism and Pharmacokinetics

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We evaluated a novel primary three-dimensional culture system for human hepatocytes using micro-space plates. The functional activity of human hepatocytes in primary culture was determined by measuring albumin secretion from hepatocytes to medium and measuring expression levels of albumin, CYP1A2 and CYP3A4 mRNA. Albumin secretion was higher in micro-space plates compared with traditional plates after 72 h of culture; the levels of albumin secretion from hepatocytes to medium in culture using micro-space plates after 96 h of culture were 2.7-fold higher than those in culture using traditional plates, and secretion of albumin in micro-space plate culture subsequently remained constant. Expression levels of albumin, CYP1A2 and CYP3A4 mRNA in the culture of hepatocytes were significantly higher using micro-space plates than using traditional plates. The inducibility of CYP1A2 and CYP3A4 mRNA after exposure to inducers in hepatocyte culture on micro-space plates was comparable to that in culture on traditional plates, while expression of CYP1A2 and CYP3A4 mRNA after exposure to inducers was higher on micro-space plates than on traditional plates. The present study demonstrates that a novel primary three-dimensional culture system of cryopreserved human hepatocytes using micro-space plates could be used for evaluating the induction of drug-metabolizing enzymes in humans. This in vitro method may thus be useful for screening the induction potency of new drug candidates.

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Section: Resultsmentioning

confidence: 59%

Section: Resultsmentioning

confidence: 99%