A Micro-Raman Spectroscopy Combing with Optical Tweezers

Yong, 张勇 Zhang; Anpei, 叶安培 Ye; Cheng, 闻丞 Wen

doi:10.3788/aos20103002.0491

Cited by 4 publications

(1 citation statement)

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“…We constructed an optical system with 532 nm laser-excited backscatter RS collection and cell bright-field imaging, as described in our previous works [ 44 , 45 ]. Briefly, the system was constructed from an inverted optical microscope (Axiovert 200, Zeiss, Oberkochen, Germany), a spectrometer (Specta Pro 2300i, Acton, Trenton, NJ, USA) with a liquid-nitrogen (LN)-cooled spectroscopic CCD (Spec-10, Princeton Instruments, Trenton, NJ, USA) with a spectral resolution of 4 cm −1 , and a wavelength of 532 nm continuous-wave laser diode (Excelsior-532-200, Spectra Physics, Santa Clara, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Sub-Cellular Dynamic Analysis of BGC823 Cells after Treatment with the Multi-Component Drug CKI Using Raman Spectroscopy

Shang

Tong

2023

IJMS

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Multi-component drugs (MCDs) can induce various cellular changes covering multiple levels, from molecular and subcellular structure to cell morphology. A “non-invasive” method for comprehensively detecting the dynamic changes of cellular fine structure and chemical components on the subcellular level is highly desirable for MCD studies. In this study, the subcellular dynamic processes of gastric cancer BGC823 cells after treatment with a multi-component drug, Compound Kushen Injection (CKI), were investigated using a homemade, high-resolution, confocal Raman spectroscopy (RS) device combined with bright-field imaging. The Raman spectra of the nucleus, cytoplasm and intracellular vesicles (0.4–1 μm) were collected simultaneously for each cell treated with CKI at different times and doses. The RS measurements showed that CKI decreased the DNA signatures, which the drug is known to inhibit. Meanwhile, the CKI-induced subcellular dynamic changes in the appearance of numerous intracellular vesicles and the deconstruction of cytoplasm components were observed and discussed. The results demonstrated that high-resolution subcellular micro-Raman spectroscopy has potential for detecting fine cellular dynamic variation induced by drugs and the screening of MCDs in cancer therapy.

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Section: Methodsmentioning

confidence: 99%

Sub-Cellular Dynamic Analysis of BGC823 Cells after Treatment with the Multi-Component Drug CKI Using Raman Spectroscopy

Shang

Tong

2023

IJMS

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Optical radiation force on a dielectric sphere of arbitrary size illuminated by a linearly polarized Airy light-sheet

Song

Sun

et al. 2020

Journal of Quantitative Spectroscopy and Radiative Transfer

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Dynamic characterization of drug resistance and heterogeneity of the gastric cancer cell BGC823 using single-cell Raman spectroscopy

Zhang

Jin

et al. 2018

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Drug resistance and heterogeneous characteristics of human gastric carcinoma cells (BGC823) under the treatment of paclitaxel (PTX) were investigated using single-cell Raman spectroscopy (RS). RS of normal and drug-resistant BGC823 cells (DR-BGC823) were collected and analyzed using arithmetic, statistic and individual spectrum analysis. The dynamic effects of paclitaxel (PTX) in normal and DR-BGC823 cells were evaluated dynamically. The RS intensity changed with PTX over time and produced distinct different results for the two types of cells. The average RS intensities of the normal BGC823 cells initially decreased and then increased under PTX treatment after 24 hours. In contrast, upon exposure to PTX, the average intensity of the DR-BGC823 cells initially increased within 12 hours and then gradually decreased and approached a steady state. The temporal variation of the typical component in the cells was analyzed by comparing the ratios between Raman bands. More importantly, the heterogeneous characteristics of the BGC823 cells under PTX treatment were quantified and clustered using hierarchical trees combined with RS intensity changes. The 'outlier' cells related to drug resistance were discriminated. The heterogeneity of the normal BGC823 cells under drug treatment gradually appeared over time, and was evaluated with the eigenvalues of principal component analysis (PCA). Our study indicates that single-cell RS may be useful in systematically and dynamically characterizing the drug response of cancer cells at the single-cell level.

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A Micro-Raman Spectroscopy Combing with Optical Tweezers

Cited by 4 publications

References 0 publications

Sub-Cellular Dynamic Analysis of BGC823 Cells after Treatment with the Multi-Component Drug CKI Using Raman Spectroscopy

Sub-Cellular Dynamic Analysis of BGC823 Cells after Treatment with the Multi-Component Drug CKI Using Raman Spectroscopy

Optical radiation force on a dielectric sphere of arbitrary size illuminated by a linearly polarized Airy light-sheet

Dynamic characterization of drug resistance and heterogeneity of the gastric cancer cell BGC823 using single-cell Raman spectroscopy

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