A micro‐scale serum neutralisation test for the detection and titration of antibodies to chicken anaemia agent ‐ prevalence of antibodies in Danish chickens

Ph, Jørgensen

doi:10.1080/03079459008418710

Cited by 16 publications

(4 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on the studies on prevalence of CIA in worldwide and Iran, it seems that most of the breeder flocks are antibody-positive against CIA, which can decrease the impotence and value of the vaccination against it (Jorgensen, 1990;Goodwin et al, 1990;Mahzounieh et al, 2005;Farhoodi et al, 2007). In this study, we could not find any anti-CAV negative-antibody breeder flocks, so we did the trial of efficacy of live Nobilis CAV P4 vaccine in positive-antibody breeder flocks.…”

Section: Discussionmentioning

confidence: 99%

“…Due to high resistance of virus to disinfecting agents, it is near to impossible to eradicate it even in specific pathogen free (SPF) flocks (Fadly et al, 1994) and the only way to prevent the disease is either with natural infection or vaccination. Maternal antibody will remain in chicken sera for 3 weeks and will protect them against disease (McNulty et al, 1988;Jorgensen, 1990;Otaki et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparison of CAV antibody titers in a vaccinated and naturally infected broiler breeder flocks

Khoshkhoo¹,

Akbariazad²,

Tashakori³

2011

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

The objective of the present work was to perform the efficacy of one attenuated live vaccine Nobilis CAV P4 (Intervet Co., Netherland) in broiler breeder and their progenies compared to naturally infected flock. The vaccine was administrated to Ross 308 broiler breeder at 6 week old through S.C. rout. A total of 352 serum samples were collected from vaccinated and unvaccinated breeder flocks (that infected naturally) from 6 to 33 weeks old and 2 times in their progenies at one-day old. Sera were analyzed using indirect Elisa kit (Synbiotic Corporation, USA) and data obtained were compared between 2 groups statistically. Due to natural infection of chicken infectious anemia (CIA) in unvaccinated breeder, mean titer in breeder and their progenies was significantly higher than vaccinated breeder and their progenies (P<0.05). The CV%s in breeders was not different significantly (P>0.05) but CV% in progeny of unvaccinated breeder flock was twice more than progeny of vaccinated flock. There were 5 and 12% of zero titers of antibody in hens and progenies in unvaccinated breeder flock respectively. All breeders from vaccinated flock were positive serologically. It seems that vaccination could be an efficient rout for eliminating susceptible birds, decreasing variation in antibody titers and induction persistent and homogenous antibody titer in progeny.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparison of CAV antibody titers in a vaccinated and naturally infected broiler breeder flocks

Khoshkhoo¹,

Akbariazad²,

Tashakori³

2011

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

show abstract

“…Infection was diagnosed on the basis of the isolated virus (Yuasa et al, 1980(Yuasa et al, , 1985Bülow, 1988;Lucio et al, 1989;Farkas et al, 1992). Serological examination showed a high content of antibodies towards chicken anemia virus (CAV) in blood sera of older poultry (Jorgensen, 1990; , as well as in many broiler chickens (Yuasa et al, 1980(Yuasa et al, , 1985Lucio et al, 1990;Dren et al, 1996). Antibodies towards CAV were also found in SPF chickens (Yuasa et al, 1985;Bülow, 1988;Nicholas et al, 1989;Godwin et al, 1990;Kapetanov et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Clinical and serological examination of a parental flock latently infected with chicken anemia virus

Kapetanov¹,

Dusan²,

Velhner³

et al. 2003

Acta vet. (Beogr.)

View full text Add to dashboard Cite

At the beginning of etiopathogenic research on chicken anaemia virus (CAV) it was thought that groups of chickens at risk for CAV infection were those coming from a parental flock infected before laying. Therefore it is important to know the level and persistence of transfered maternal antibodies (MaAt) and to measure specific antibody development during rearing. The goal of this research was to assess the necessity for prophylactic measures by determining the humoral immune response to CAV and any clinical changes in breeder chickens. Chickens from the parental Hybro flock were examined from the first day until the end of production. Maternal antibodies for CAV, which were present initially, were not detected at 4 weeks old. At 6 weeks old specific antibodies for CAV were found in 45% of the serum samples. These antibodies increased until the 18th week when the experiment was terminated. The state of health of the parental flock in the period when MaAt antibodies could not be detected and until specific antibodies appeared did not differ significantly. The results of these investigations are the first evidence of CAV infection in Yugoslavia, based on serological examination

show abstract

“…The serological diagnosis is based on virus neutralization (VN) test (5,6,7,8), indirect immunofluorescence (IIFA) (6,9,10,11) and indirect, or blocking ELISAs (12,13,14). Commercial ELISA kits are used most commonly in a large scale monitoring of the immune status of breeder flocks.…”

Section: Introductionmentioning

confidence: 99%

Development of Immunofluorescence-Based Virus Neutralization Test for the Detection of Antibodies to Chicken Anemia Virus

Simeonov

Doumanova

2005

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

A novel immunoflorescence-virus neutralization microtest (VNIF), based on the immunofluorescent detection of virus antigens in cells, inoculated with virus/antibody mixtures was developed for determination of neutralizing antibodies against chicken anemia virus (CAV). For this purpose serial dilutions of tested sera were incubated with 10 5 TCID 50 of Del Rose strain of CAV and neutralizing mixtures incubated with MDCC-MSB1 cells in 96-well microtest plates. The infected cell cultures were subpassaged once, and 48h thereafter tested for the presence of residual virus in neutralizing mixtures using direct immunofluorescent test (IF). Two sera from Leghorn line 15 I hens, reared at separated conditions and vaccinated with commercial vaccine CAV P4, four other sera from chickens from the same line and 5 serum samples from commercial broiler chickens were tested comparatively by both virus neutralization (VN) and VNIF tests. The sensitivity of VNIF was shown to be greater than a conventional VN test, which rely on subjective recognition of cytopathic effect (CPE), and viral activity was detected in wells, inoculated with endpoint dilutions of virus-serum mixture and judged as CPE-negative. The VNIF system have the advantages to be more objective and rapid than the classical VN and could successfully be used for large-scale serological monitoring of CAV infection.

show abstract

A micro‐scale serum neutralisation test for the detection and titration of antibodies to chicken anaemia agent ‐ prevalence of antibodies in Danish chickens

Cited by 16 publications

References 19 publications

Comparison of CAV antibody titers in a vaccinated and naturally infected broiler breeder flocks

Comparison of CAV antibody titers in a vaccinated and naturally infected broiler breeder flocks

Clinical and serological examination of a parental flock latently infected with chicken anemia virus

Development of Immunofluorescence-Based Virus Neutralization Test for the Detection of Antibodies to Chicken Anemia Virus

Contact Info

Product

Resources

About