A micro-volume viscosity measurement technique based on μPIV diffusometry

Sie, Yue Syun; Chuang, Huey-Ru

doi:10.1007/s10404-013-1219-4

Cited by 19 publications

(29 citation statements)

References 23 publications

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“…However, the sinusoidal waveform is typically a theoretical model, which may inevitably introduce deviations from the reality. Our findings 8 and prior research 2,9 have shown that C. elegans tends to vary its swimming gait in response to the ambient viscosity. Nevertheless, the same theory was later adopted by some research groups to study the nematode neurobiology and behavior 10 phenotype.…”

Section: Introductionsupporting

confidence: 73%

“…A medium with viscosity of 10 mPa s was made by mixing dextran (FL-95771/MW ¼ 2 000 000, Sigma) with the NGM buffer to achieve a 6% dextran solution. Numerous studies 2,8,9,31 have reported that C. elegans is able to sense the ambient viscosity change and make a behavioral response according to the coordination between sensory and motor neurons. A representative trajectory of movement and a relationship of power and propulsion similar to the previous N2 and kin-2 are depicted in Figs.…”

Section: Effects Of Strain and Liquid Viscosity To Motility Changesmentioning

confidence: 99%

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Characterizations of kinetic power and propulsion of the nematode Caenorhabditis elegans based on a micro-particle image velocimetry system

2014

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Quantifying the motility of micro-organisms is beneficial in understanding their biomechanical properties. This paper presents a simple image-based algorithm to derive the kinetic power and propulsive force of the nematode Caenorhabditis elegans. To avoid unnecessary disturbance, each worm was confined in an aqueous droplet of 0.5 ll. The droplet was sandwiched between two glass slides and sealed with mineral oil to prevent evaporation. For motion visualization, 3-lm fluorescent particles were dispersed in the droplet. Since the droplet formed an isolated environment, the fluid drag and energy loss due to wall frictions were associated with the worm's kinetic power and propulsion. A microparticle image velocimetry system was used to acquire consecutive particle images for fluid analysis. The shorttime interval (Dt < 20 ms) between images enabled quasi real-time measurements. A numerical simulation of the flow in a straight channel showed that the relative error of this algorithm was significantly mitigated as the image was divided into small interrogation windows. The time-averaged power and propulsive force of a N2 adult worm over three swimming cycles were estimated to be 5.2 6 3.1 pW and 1.0 6 0.8 nN, respectively. In addition, a mutant, KG532 [kin-2(ce179) X], and a wild-type (N2) worm in a viscous medium were investigated. Both cases showed an increase in the kinetic power as compared with the N2 worm in the nematode growth medium due to the hyperactive nature of the kin-2 mutant and the high viscosity medium used. Overall, the technique deals with less sophisticated calculations and is automation possible. V C 2014 AIP Publishing LLC.

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Section: Introductionsupporting

confidence: 73%

Section: Effects Of Strain and Liquid Viscosity To Motility Changesmentioning

confidence: 99%

Characterizations of kinetic power and propulsion of the nematode Caenorhabditis elegans based on a micro-particle image velocimetry system

2014

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“…Worm motility can be observed by counting the cycle of a worm’s head thrashing trajectory, which is called “body bends.” Prior studies13141516 have shown that C. elegans performs swimming and crawling gaits at low and high medium viscosities, respectively. Therefore, we can expect worms to show vigorous locomotion in the flow chamber.…”

Section: Resultsmentioning

confidence: 99%

Exercise in an electrotactic flow chamber ameliorates age-related degeneration in Caenorhabditis elegans

Chuang

Kuo

Lee

et al. 2016

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Degeneration is a senescence process that occurs in all living organisms. Although tremendous efforts have been exerted to alleviate this degenerative tendency, minimal progress has been achieved to date. The nematode, Caenorhabditis elegans (C. elegans), which shares over 60% genetic similarities with humans, is a model animal that is commonly used in studies on genetics, neuroscience, and molecular gerontology. However, studying the effect of exercise on C. elegans is difficult because of its small size unlike larger animals. To this end, we fabricated a flow chamber, called “worm treadmill,” to drive worms to exercise through swimming. In the device, the worms were oriented by electrotaxis on demand. After the exercise treatment, the lifespan, lipofuscin, reproductive capacity, and locomotive power of the worms were analyzed. The wild-type and the Alzheimer’s disease model strains were utilized in the assessment. Although degeneration remained irreversible, both exercise-treated strains indicated an improved tendency compared with their control counterparts. Furthermore, low oxidative stress and lipofuscin accumulation were also observed among the exercise-treated worms. We conjecture that escalated antioxidant enzymes imparted the worms with an extra capacity to scavenge excessive oxidative stress from their bodies, which alleviated the adverse effects of degeneration. Our study highlights the significance of exercise in degeneration from the perspective of the simple life form, C. elegans.

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“…2A)2340. The observation area was isolated by a circular groove, ensuring that the drop forms a hocky-pocket shape, to define the geometric dimension of a sample drop on the chip.…”

Section: Methodsmentioning

confidence: 99%

Simultaneous and quantitative monitoring of co-cultured Pseudomonas aeruginosa and Staphylococcus aureus with antibiotics on a diffusometric platform

Chung

Wang

Chuang

2017

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Successful treatments against bacterial infections depend on antimicrobial susceptibility testing (AST). However, conventional AST requires more than 24 h to obtain an outcome, thereby contributing to high patient mortality. An antibiotic therapy based on experiences is therefore necessary for saving lives and escalating the emergence of multidrug-resistant pathogens. Accordingly, a fast and effective drug screen is necessary for the appropriate administration of antibiotics. The mixed pathogenic nature of infectious diseases emphasizes the need to develop an assay system for polymicrobial infections. On this basis, we present a novel technique for simultaneous and quantitative monitoring of co-cultured microorganisms by coupling optical diffusometry with bead-based immunoassays. This simple integration simultaneously achieves a rapid AST analysis for two pathogens. Triple color particles were simultaneously recorded and subsequently analyzed by functionalizing different fluorescent color particles with dissimilar pathogen-specific antibodies. Results suggested that the effect of the antibiotic, gentamicin, on co-cultured Pseudomonas aeruginosa and Staphylococcus aureus was effectively distinguished by the proposed technique. This study revealed a multiplexed and time-saving (within 2 h) platform with a small sample volume (~0.5 μL) and a low initial bacterial count (50 CFU per droplet, ~105 CFU/mL) for continuously monitoring the growth of co-cultured microorganisms. This technique provides insights into timely therapies against polymicrobial diseases in the near future.

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A micro-volume viscosity measurement technique based on μPIV diffusometry

Cited by 19 publications

References 23 publications

Characterizations of kinetic power and propulsion of the nematode Caenorhabditis elegans based on a micro-particle image velocimetry system

Characterizations of kinetic power and propulsion of the nematode Caenorhabditis elegans based on a micro-particle image velocimetry system

Exercise in an electrotactic flow chamber ameliorates age-related degeneration in Caenorhabditis elegans

Simultaneous and quantitative monitoring of co-cultured Pseudomonas aeruginosa and Staphylococcus aureus with antibiotics on a diffusometric platform

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