A microanalytical technique for the estimation of fluoride in rat molar enamel

Retief, D.H.; Navia, Juan M.; Lopez, Hady

doi:10.1016/0003-9969(77)90156-x

Cited by 19 publications

(9 citation statements)

References 15 publications

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“…Although several investigators have found a fluoride gradient (Riethe, 1968;Mellberg and Larson, 1978;Regolati et al, 1974), it is difficult to compare the results from one investigation with those from another, because the thicknesses of rat enamel removed in the various studies were not the same. However, our results agree closely with those of Retief (1977), who removed comparable amounts of enamel. Seven days elapsed in the first study between the last rinse and death of the animal, during which time most of the loosely-bound fluoride would have cleared from the oral cavity.…”

Section: Discussionsupporting

confidence: 93%

Effects of Sequential Calcium Phosphate-Fluoride Rinses on Dental Plaque, Staining, Fluoride Uptake, and Caries in Rats

et al. 1984

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Oral rinses which included (1) an acidic calcium phosphate solution containing 0.7 M Ca, 1.9 M PO4, and saturated with respect to CaHPO4 . 2H2O, and with a pH of 2.0, followed by (2) a 0.52 M fluoride solution, from NaF or SnF2, were provided to rats once daily for seven days. The investigation consisted of two studies: In the first study, the amounts of dental plaque on the tooth surfaces and fluoride concentrations in the outer enamel were assessed seven days after the last treatment; in the second study, the extent of dental caries was evaluated seven weeks after the last treatment. All rinse sequences containing fluoride provided significant caries protection. The acidic calcium phosphate treatment markedly enhanced the ability of the enamel to acquire fluoride without change of surface morphology. Only the rinse sequences that included stannous fluoride showed significant plaque suppression.

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Section: Discussionsupporting

confidence: 93%

Effects of Sequential Calcium Phosphate-Fluoride Rinses on Dental Plaque, Staining, Fluoride Uptake, and Caries in Rats

et al. 1984

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“…We feel, however,, that these did not essentially modify the results because of the poor accessibility of the fissures and the small surface area ofthe cuspal dentin. Our method was not as sophisticated as that of RETIEF et al (28); the molar surface area was exposed to acid and thus the etching depth could not be satisfactorily calculated. Bearing in mind the different modes of fluoride administration, the dissolution procedure and the technique of analysis, our fluoride values agree reasonably with those of LARSON et al (15) and RETIEF et at.…”

Section: Enamel Solubilitymentioning

confidence: 99%

Caries reduction in rats through F + Mg supplementation of dietary sucrose with observations on bone mineral density and soft and hard tissue minerals

Luoma

Koskinen

Olkkonen

et al. 1982

European J Oral Sciences

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Caries reduction in rats through F + Mg supplementation of dietary sucrose with observations on bone mineral density and soft and hard tissue minerals. Scand. J. Dent. Res. 1982: 90: 345^353.Abstract -Two control diets with different sucrose contents (44% and 22%) were given to weanling rats for 55 d. The test diets were .sopplemenited with an F + Mg combination, 75 parts/10^ F as NaF+ 50 parts/10'' Mg as MgO and 38 parts/JO^ F + 25 parts/10*^ Mg, respectively, i.e. using the same suppSement/sucrose ratio in each test diet. The sopplementation in each test diet caused the same percentage fissure caries reduction, but the absolute mean caries scores were higher in the high-sucrose groups than in the respective low-sucrose groups,. Enamel solubility was reduced by the supplemenladon. Despite the high iliuoride supplementation, no untoward effects were recorded in growth and in aortiC;k eart, and renal calcium contents. Bone mineral density was reduced in the low-sucrose controls and slightly reduced in the high-sucrose F+Mg supplemented rats, as compared with the high-sucrose controls.

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“…An acid etch microbiopsy technique was used to determine the enamel and cementum fluoride uptake [Retief et al, 1977;Tantbirojn et al, 1992]. In the present study the baseline enamel and cementum fluoride concentrations were subtracted from the experimental fluoride concentrations to determine the fluoride uptake.…”

Section: Fluoride Uptake By Enamel and Cementummentioning

confidence: 99%

In vitro Enamel and Cementum Fluoride Uptake from Three Fluoride–Containing Composites

et al. 1998

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The incorporation of fluoride into restorations is desirable because of its cariostatic action. The purpose of this study was to determine fluoride release and fluoride uptake by enamel and cementum from three visible light–cured fluoride–containing composites. Seven circular discs of each composite were prepared and the amount of fluoride released into distilled water was determined at daily intervals for 14 days and then after 30, 60 and 90 days. The fluoride concentration in enamel and cementum was determined in three successive depths by an acid etch biopsy procedure. The composite slabs were made and ligated to the enamel and cementum surfaces and the teeth were immersed in synthetic saliva for 7 days. After removal of the composite slabs, three successive biopsies were again performed. Then the teeth were immersed in 1 M KOH for 24 h and similar biopsies done. The fluoride concentrations were adjusted to standardized depths of 10μm, and the total and bound fluoride uptake calculated. The amounts of fluoride released were significantly different among the three composites. The fluoride released decreased sharply after 1 day and then gradually reached a plateau. As for the enamel and cementum fluoride uptake, FluorEver showed the largest uptake followed by FluoroCore and then Pertac–Hybrid.

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A microanalytical technique for the estimation of fluoride in rat molar enamel

Cited by 19 publications

References 15 publications

Effects of Sequential Calcium Phosphate-Fluoride Rinses on Dental Plaque, Staining, Fluoride Uptake, and Caries in Rats

Effects of Sequential Calcium Phosphate-Fluoride Rinses on Dental Plaque, Staining, Fluoride Uptake, and Caries in Rats

Caries reduction in rats through F + Mg supplementation of dietary sucrose with observations on bone mineral density and soft and hard tissue minerals

In vitro Enamel and Cementum Fluoride Uptake from Three Fluoride–Containing Composites

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