A microbial method using whole cells of Thiobacillus thiooxidans for measuring sulphate in waters

Nakamura, Kazuo; Yudiarto, M. A.; Kaneko, Naoko; Kurosawa, Hiroshi; Amano, Yoshifumi

doi:10.1007/s002530051128

Cited by 6 publications

(3 citation statements)

References 11 publications

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“…For the enrichment of the different bacterial species from the sulphide ore studied, were used 250 mL Erlenmeyer flasks with 100 mL of the culture medium for each bacterial species described by the following authors: Leptospirillum ferrooxidans ( Battaglia et al , 1994 ); Acidithiobacillus ferrooxidans , ( Tuovinen and Kelly, 1973 ); Leptospirillum ferriphilum at a temperature of 45 °C ( Battaglia et al , 1994 ); Acidithiobacillus thiooxidans ( Nakamura et al , 1997 ). Then, the flasks with enrichment media for the various species were incubated in a thermo regulated shaker (Lab Line brand model No.…”

Section: Methodsmentioning

confidence: 99%

Bacterial consortium for copper extraction from sulphide ore consisting mainly of chalcopyrite

Romo¹,

Weinacker²,

Zepeda³

et al. 2013

Braz. J. Microbiol.

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The mining industry is looking forward for bacterial consortia for economic extraction of copper from low-grade ores. The main objective was to determine an optimal bacterial consortium from several bacterial strains to obtain copper from the leach of chalcopyrite. The major native bacterial species involved in the bioleaching of sulphide ore (Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans, Leptospirillum ferrooxidans and Leptospirillum ferriphilum) were isolated and the assays were performed with individual bacteria and in combination with At. thiooxidans. In conclusion, it was found that the consortium integrated by At. ferrooxidans and At. thiooxidans removed 70% of copper in 35 days from the selected ore, showing significant differences with the other consortia, which removed only 35% of copper in 35 days. To validate the assays was done an escalation in columns, where the bacterial consortium achieved a higher percentage of copper extraction regarding to control.

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Section: Methodsmentioning

confidence: 99%

Bacterial consortium for copper extraction from sulphide ore consisting mainly of chalcopyrite

Romo¹,

Weinacker²,

Zepeda³

et al. 2013

Braz. J. Microbiol.

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“…As a new desulfurization method, the microorganism desulfurization way drew more attention in various countries [10][11][12][13][14][15][16][17][18] , because it had appeared to have lower cost, higher yield of second-produces, and higher desulfurization efficiency. However, it had some shortages, such as longer desulfurization time, higher possibilities of aberrance in desulfurization processes, and more complicated desulfurization mechanism in desulfurization processes.…”

Section: Introductionmentioning

confidence: 99%

Isolation and identification of the thermophilic alkaline desulphuricant strain

Zhang

Chen

et al. 2008

Sci. China Ser. B-Chem.

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A desulfurization strain that belongs to the thermophilic alkaline desulphuricant is designated as strain GDJ-3 and isolated from Inner Mongolia, China. The colony of the strain shows tiny, yellow, or white-yellow, and it becomes henna with the protracting of cultivated time. The cells are bacilliform (0.3 -0.6 × 1.0-1.2 μm), motive, and Gram negative. The strain GDJ-3 is able to utilize respectively the thiosulphate, sulfate, sulfite, or sulfide as sulfur source, utilize the carbon dioxide as the carbon source, and utilize the ammonium or nitrate as the nitrogen source. According to GenBank data, 16s RNA results of GDJ-3 are in good agreement with Alpha proteobacterrium sp. (97%) and Ochrobactrum sp. (98%). For GDJ-3, the optimum growth temperature is at 45℃, the optimum pH is at 8.5-8.8, and the optimum rocking speed of sorting table is at 150 r/min. Under the optimum culture condition, the cells of the strain can live for about 18 h. In the desulfurization solution, which is prepared according to the composition of DDS solution, the objectionable constituents of sodium thiosulphate and sodium sulfide were added factitiously, and the bacterial cell concentration was set at 10 7 /mL. After the regeneration of the above desulfurization solution by the strain cells, the concentration of sodium thiosulphate was decreased by 14.75 g/L (percentage loss of content 13.21%), the concentration of sodium sulfide was decreased by 0.76 g/L (percentage loss of content 87.36%) in the desulfurization solution in 9.5 hours, and sulfur appeared. Maybe, this kind of strain can be used as the regeneration's bacterial source of DDS solution.thermophilic alkaline desulphurican, thiobacillus, strain identification, DDS catalyst

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“…There are various methods available for the determination of sulfate (Crompton, 1996). Examples include a titrimetric (Masadome and Asano, 1999), gravimetric (Prada et al, 1996;Vieira et al, 1998;Staden and Taljaard, 1996), colorimetric (Nakamura et al, 1997), spectrophotometric (Molinero et al, 1996), Raman and IR methods (Lijour et al, 1994;Murata et al, 1997), as well as ion chromatography (Micheel et al, 1998;Miura et al, 1997), and ion selective electrode-based methods (Li et al, 1999). Some of these methods are cumbersome, time-consuming, and lack selectivity.…”

Section: Introductionmentioning

confidence: 99%

Rationally designed fluorescently labeled sulfate‐binding protein mutants: Evaluation in the development of a sensing system for sulfate

Shrestha

Salins

Ensor

et al. 2002

Biotech & Bioengineering

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Periplasmic binding proteins from E. coli undergo large conformational changes upon binding their respective ligands. By attaching a fluorescent probe at rationally selected unique sites on the protein, these conformational changes in the protein can be monitored by measuring the changes in fluorescence intensity of the probe which allow the development of reagentless sensing systems for their corresponding ligands. In this work, we evaluated several sites on bacterial periplasmic sulfate-binding protein (SBP) for attachment of a fluorescent probe and rationally designed a reagentless sensing system for sulfate. Eight different mutants of SBP were prepared by employing the polymerase chain reaction (PCR) to introduce a unique cysteine residue at a specific location on the protein. The sites Gly55, Ser90, Ser129, Ala140, Leu145, Ser171, Val181, and Gly186 were chosen for mutagenesis by studying the three-dimensional X-ray crystal structure of SBP. An environment-sensitive fluorescent probe (MDCC) was then attached site-specifically to the protein through the sulfhydryl group of the unique cysteine residue introduced. Each fluorescent probe-conjugated SBP mutant was characterized in terms of its fluorescence properties and Ser171 was determined to be the best site for the attachment of the fluorescent probe that would allow for the development of a reagentless sensing system for sulfate. Three different environment-sensitive fluorescent probes (1,5-IAEDANS, MDCC, and acylodan) were studied with the SBP171 mutant protein. A calibration curve for sulfate was constructed using the labeled protein and relating the change in the fluorescence intensity with the amount of sulfate present in the sample. The detection limit for sulfate was found to be in the submicromolar range using this system. The selectivity of the sensing system was demonstrated by evaluating its response to other anions. A fast and selective sensing system with detection limits for sulfate in the submicromolar range was developed.

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A microbial method using whole cells of Thiobacillus thiooxidans for measuring sulphate in waters

Cited by 6 publications

References 11 publications

Bacterial consortium for copper extraction from sulphide ore consisting mainly of chalcopyrite

Bacterial consortium for copper extraction from sulphide ore consisting mainly of chalcopyrite

Isolation and identification of the thermophilic alkaline desulphuricant strain

Rationally designed fluorescently labeled sulfate‐binding protein mutants: Evaluation in the development of a sensing system for sulfate

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